Changhai Zhou
Jilin University
P70-S6 Kinase 1Messenger RNATransforming growth factorUnfolded protein responsemicroRNACellMolecular biologyEndoplasmic reticulumChemistryApoptosisSuperoxide dismutaseSOD2Oxidative stressMastitisFatty acid synthesisZearalenoneTriglycerideCell cycleFatty acid synthaseReactive oxygen speciesViability assayGlutathioneTransfectionFlow cytometryBiochemistryCell growthHsp70Sterol regulatory element-binding proteinBiologyImmune systemMicrobiologyCell biology
17Publications
5H-index
64Citations
Publications 17
Newest
#1Nan Wang (JLU: Jilin University)
#2Changhai Zhou (JLU: Jilin University)H-Index: 5
Last. Xu Zhou (JLU: Jilin University)H-Index: 12
view all 8 authors...
Reproductive performance is a key factor in determining the profitability of dairy farm, which is affected by many factors such as environment and diseases. Mastitis is a common and important disease, which has caused huge economic losses to the dairy industries worldwide. Mammary gland infection causes immune responses, resulting in the abnormal secretion of cytokines and hormones and abnormal function of the reproductive system such as the ovary, corpus luteum, uterus, and embryo. Cows with ma...
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#1Yurong Fu (JLU: Jilin University)H-Index: 4
#2Yongcheng Jin (JLU: Jilin University)H-Index: 8
Last. Yue Tian (JLU: Jilin University)
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Zearalenone (ZEA) is a mycotoxin of the Fusarium genus that can cause endoplasmic reticulum (ER) stress and Apoptosis in bovine mammary epithelial cells (MAC-T). Polydatin (PD), a glycoside purified from Polygonum cuspidatum, has antioxidant properties. This study aimed to explore whether PD can alleviate ZEA-induced damage on bovine mammary epithelial cells (MAC-T). We found that incasing the concentration of ZEA (0, 7.5, 15, 30, 60, 90, 120, and 240 μM) gradually decreased the cell viability. ...
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#1Jing Zhang (JLU: Jilin University)H-Index: 5
#2Junmei Wang (Sichuan Agricultural University)H-Index: 4
Last. Yongcheng Jin (JLU: Jilin University)H-Index: 8
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Abstract More and more studies have showed that tricothecene mycotoxin, deoxynivalenol (DON) caused cytotoxicity in mammary alveolar cells-large T antigen cells (MAC-T). Therefore, research on reducing the cytotoxicity of DON has gradually attracted attention. In this study, we aim to explore the potential of pterostilbene (PTE) to protect MAC-T cells from DON-induced oxidative stress and inflammatory response. MAC-T cells were treated with 0.25 μg/mL DON or 2.0504 μg/mL PTE or 0.25 μg/mL DON an...
1 CitationsSource
#1Ming-Qi WangH-Index: 2
#2Changhai Zhou (JLU: Jilin University)H-Index: 5
Last. Bao Yuan (JLU: Jilin University)H-Index: 11
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ABSTRACT The fat content of milk determines the quality of milk, and triglycerides are the major components of milk fat. Milk fat synthesis is regulated by many factors. Lipopolysaccharide (LPS) has been shown to inhibit milk fat synthesis in bovine mammary epithelial cells, but research on the underlying mechanisms has been limited. MicroRNA (miRNA) are involved in many physiological processes, but there have been few studies on their regulation in milk fat synthesis. In this study, we aimed to...
1 CitationsSource
#1Xian-Dong Liao (JLU: Jilin University)H-Index: 1
#2Changhai Zhou (JLU: Jilin University)H-Index: 5
Last. Shengli Li (CAU: China Agricultural University)H-Index: 11
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OBJECTIVE: Caseins and fatty acids of milk are synthesized and secreted by the epithelial cells of the mammary gland. All-trans retinoic acid (ATRA), an active metabolite of vitamin A, has been shown to promote mammary development. This study was conducted to determine the effect of ATRA on casein synthesis and fatty acid composition in MAC-T cells. METHODS: MAC-T cells were allowed to differentiate for 4 d, treated with ATRA (0, 1.0, 1.5, and 2.0 muM), and incubated for 3 d. We analyzed the fat...
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#1Rui Wang (JLU: Jilin University)H-Index: 4
#2Hao Yu (JLU: Jilin University)H-Index: 8
Last. Jing Zhang (JLU: Jilin University)H-Index: 5
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Grape pomace (GP) is an abundant by-product from wine production and is rich in phenolic compounds, unsaturated fatty acids, dietary fibre and beneficial bacteria. In this study, weaned piglets were fed a basic diet supplemented with 5% GP for 4 weeks. Compared with those in the control (CON) group, it was found that the proportion of Lactobacillus delbrueckii, Olsenella umbonata and Selenomonas bovis in the caecum and the villus height and villus height/crypt depth ratio (VCR) of the jejunum we...
1 CitationsSource
#1Wanqi Zhong (JLU: Jilin University)H-Index: 1
#2Jinglin Shen (JLU: Jilin University)H-Index: 4
Last. Yongcheng Jin (JLU: Jilin University)H-Index: 8
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Camellia (Camellia oleifera Abel.) seed oil is a commonly used edible oil of China. In ancient Chinese literature, it is mentioned to be helpful for postpartum repair and lactation in women. Research on camellia seed oil (CO) as a feed additive for dairy cattle is less. We investigated the effect of CO on the expression of milk fat and protein syntheses-related genes in differentiated bovine mammary epithelial cells (MAC-T) using soybean oil (SO) as the control. The results showed that CO increa...
3 CitationsSource
#1Tinghao Huang (JLU: Jilin University)H-Index: 2
#2Changhai Zhou (JLU: Jilin University)H-Index: 5
Last. Liancheng Lei (JLU: Jilin University)H-Index: 19
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Transforming growth factor (TGF)-β1 is a multifunctional cytokine that plays an important role in regulating immune cell proliferation. We speculate that high expression of TGF-β1 may affect the im...
1 CitationsSource
#1Xin Wang (JLU: Jilin University)H-Index: 4
#2Hao Yu (JLU: Jilin University)H-Index: 8
Last. Jing Zhang (JLU: Jilin University)H-Index: 5
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Abstract Mounting evidence has shown that zearalenone (ZEA) can have toxic effects on the intestinal epithelial cells (IECs) of mammals and humans, but the mechanism of ZEA-induced toxicity on IECs is unclear. The aim of this study was to reveal the mechanism of action of ZEA on intestinal epithelial cells via RNA-seq technology. We measured the effects of ZEA on the viability and lactate dehydrogenase (LDH) activity of the pig intestinal epithelial cell line J2 (IPEC-J2). The results showed ZEA...
4 CitationsSource
#1Meina Zhang (JLU: Jilin University)H-Index: 1
#2Yanyi Che (JLU: Jilin University)H-Index: 7
Last. Liancheng Lei (Yangtze University)H-Index: 19
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Abstract Mastitis is a disease that affects dairy cattle and causes a decline in milk quality as well as economic loss worldwide. TGF-β1 levels are usually increased during mastitis; however, it is unknown whether TGF-β1 is involved in bovine mastitis. Therefore, this study evaluated the effects of TGF-β1 on the susceptibility of bovine mammary epithelial cells (BMECs) to Staphylococcus aureus (S. aureus). The results revealed that S. aureus adhesion to and invasion of BMECs was significantly in...
1 CitationsSource