Linda N. Liu
P70-S6 Kinase 1Cell cycle checkpointCyclin-dependent kinaseInternal medicineAutocrine signallingEndocrinologyCyclin AApoptosisGrowth factorSirolimusGrowth inhibitionTOR Serine-Threonine KinasesEukaryotic initiation factor 4E bindingCell cycleProgrammed cell deathRPTORCancer researchCell growthCell culturePI3K/AKT/mTOR pathwayBiologyCell biologymTORC1
Publications 3
#1Shile Huang (St. Jude Children's Research Hospital)H-Index: 56
#2Linda N. LiuH-Index: 3
Last. Peter J. HoughtonH-Index: 98
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The relationship between G 1 checkpoint function and rapamycininduced apoptosis was examined using two human rhabdomyosarcoma cell lines, Rh1 and Rh30, that express mutated p53 alleles. Serum-starved tumor cells became apoptotic when exposed to rapamycin, but were completely protected by expression of a rapamycin-resistant mutant mTOR. Exposure to rapamycin (100 ng/ml) for 24 h significantly increased the proportion of Rh1 and Rh30 cells in G 1 phase, although there were no significant changes i...
195 Citations
#1Hajime Hosoi (St. Jude Children's Research Hospital)H-Index: 33
#2Michael B. DillingH-Index: 8
Last. Peter J. HoughtonH-Index: 98
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The mammalian target of rapamycin (mTOR) has been shown to link growth factor signaling and posttranscriptional control of translation of proteins that are frequently involved in cell cycle progression. However, the role of this pathway in cell survival has not been demonstrated. Here, we report that rapamycin, a specific inhibitor of mTOR kinase, induces G 1 cell cycle arrest and apoptosis in two rhabdomyosarcoma cell lines (Rh1 and Rh30) under conditions of autocrine cell growth. To examine th...
271 Citations
#1Hajime Hosoi (Kyoto Prefectural University of Medicine)H-Index: 33
#2Michael B. DillingH-Index: 8
Last. Peter J. HoughtonH-Index: 98
view all 9 authors...
Rapamycin is a potent cytostatic agent that arrests cells in the G1 phase of the cell cycle. The relationships between cellular sensitivity to rapamycin, drug accumulation, expression of mammalian target of rapamycin (mTOR), and inhibition of growth factor activation of ribosomal p70S6 kinase (p70S6k) and dephosphorylation of pH acid stable protein I (eukaryotic initiation factor 4E binding protein) were examined. We show that some cell lines derived from childhood tumors are highly sensitive to...
157 CitationsSource