Carla Dessels
University of Pretoria
Cell therapyHaematopoiesisAdipogenesisDownregulation and upregulationTranscriptomeStromal cellAdipose tissueCryopreservationMesenchymal stem cellImmunologyHematopoietic stem cell transplantationFetal bovine serumStromal vascular fractionStem cellTransplantationBioinformaticsMedicinePlacenta cord bankingBiologyCell biology
10Publications
5H-index
134Citations
Publications 8
Newest
#1Chrisna Durandt (University of Pretoria)H-Index: 10
#2Carla Dessels (University of Pretoria)H-Index: 5
Last. Michael S. Pepper (University of Pretoria)H-Index: 73
view all 5 authors...
Multipotent adipose-derived stromal/stem cells (ASCs) are candidates for use in cellular therapies for the treatment of a variety of conditions/diseases. Ex vivo expansion of freshly isolated ASCs may be necessary prior to clinical application to ensure that clinically relevant cell numbers are administered during treatment. In addition, cryopreserving cells at early passages allows for storage of freshly isolated cells for extended periods of time before expanding these cells for clinical usage...
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#1Carla Dessels (University of Pretoria)H-Index: 5
#2Melvin A Ambele (University of Pretoria)H-Index: 5
Last. Michael S. Pepper (University of Pretoria)H-Index: 73
view all 3 authors...
Background For adipose-derived stromal cells (ASCs) to be safe for use in the clinical setting, they need to be prepared using good manufacturing practices (GMPs). Fetal bovine serum (FBS), used to expand ASCs in vitro in some human clinical trials, runs the risk of xenoimmunization and zoonotic disease transmission. To ensure that GMP standards are maintained, pooled human platelet lysate (pHPL) has been used as an alternative to FBS. ASCs proliferate more rapidly in pHPL than in FBS, with no s...
2 CitationsSource
#1Carla Dessels (University of Pretoria)H-Index: 5
#2Michael S. Pepper (University of Pretoria)H-Index: 73
Adipose-derived stromal cells (ASCs) are becoming increasingly attractive as cellular therapy products. Their differentiation potential, the secretion of growth and differentiation factors, and the...
1 CitationsSource
#1Carla Dessels (University of Pretoria)H-Index: 5
#2Chrisna Durandt (University of Pretoria)H-Index: 10
Last. Michael S. Pepper (University of Pretoria)H-Index: 73
view all 3 authors...
Grants from the South African Medical Research Council Flagship Awards Project SAMRC-RFA-UFSP- 01-2013/STEM CELLS, the SAMRC Extramural Unit for Stem Cell Research and Therapy and the Institute for Cellular and Molecular Medicine of the University of Pretoria. The National Research Foundation of South Africa also provided funding.
12 CitationsSource
#1Carla Dessels (South African Medical Research Council)H-Index: 5
#2Marco Alessandrini (South African Medical Research Council)H-Index: 7
Last. Michael S. Pepper (South African Medical Research Council)H-Index: 73
view all 3 authors...
The Medical Research Council of South Africa in terms of (a) the MRC’s Flagships Awards Project SAMRC-RFA-UFSP-01–2013/STEM CELLS and (b) the Extramural Unit for Stem Cell Research and Therapy. Funding was also provided by the Institute for Cellular and Molecular Medicine, Faculty of Health Sciences, University of Pretoria.
26 CitationsSource
#1Carla Dessels (South African Medical Research Council)H-Index: 5
#2Marnie Potgieter (South African Medical Research Council)H-Index: 9
Last. Michael S. Pepper (South African Medical Research Council)H-Index: 73
view all 3 authors...
Adipose-derived stromal cells (ASCs) are being used extensively in clinical trials. These trials require that ASCs are prepared using good manufacturing procedures (GMPs) and are safe for use in humans. The majority of clinical trials in which ASCs are expanded make use of fetal bovine serum (FBS). While FBS is used traditionally in the research setting for in vitro expansion, it does carry the risk of xenoimmunization and zoonotic transmission when used for expanding cells destined for therapeu...
25 CitationsSource
#1Melvin A Ambele (University of Pretoria)H-Index: 5
#2Carla Dessels (University of Pretoria)H-Index: 5
Last. Michael S. Pepper (University of Pretoria)H-Index: 73
view all 4 authors...
The South African Medical Research Council in terms of the SAMRC's Flagship Award Project SAMRC-RFAUFSP- 01-2013/STEM CELLS, the SAMRC Extramural Stem Cell Research and Therapy Unit, the National Research Foundation of South Africa (grant no. 86942), the National Health Laboratory Services Research Trust (grant no. 94453) and the Institute for Cellular and Molecular Medicine of the University of Pretoria.
52 CitationsSource
#1Chrisna Durandt (South African Medical Research Council)H-Index: 10
#2Fiona A. Van Vollenstee (South African Medical Research Council)H-Index: 4
Last. Michael S. Pepper (South African Medical Research Council)H-Index: 73
view all 8 authors...
The ability of mesenchymal stromal cells (MSCs) to differentiate into adipocytes provides a cellular model of human origin to study adipogenesis in vitro. One of the major challenges in studying adipogenesis is the lack of tools to identify and monitor the differentiation of various subpopulations within the heterogeneous pool of MSCs. Cluster of differentiation (CD)36 plays an important role in the formation of intracellular lipid droplets, a key characteristic of adipocyte differentiation/matu...
22 CitationsSource