Anke Seifert
RWTH Aachen University
Loss functionLigand (biochemistry)C-C chemokine receptor type 7Chemokine receptorCCR1ChemokineCXCL11Innate immune systemInternalizationPathologyCellPsychologyPhagocytosisHomology (biology)EndogenyNeuroscienceReceptorGene knockdownChemistrySmall hairpin RNAAcquired immune systemIn vitroIn vivoIn silicoIsolation (microbiology)Orbitofrontal cortexProteaseInflammationAstrocyteHeparinLungDisintegrinADAM10Functional magnetic resonance imagingADAM8Serotonin reuptake inhibitorCingulate cortexPrecuneusPolymorphonuclear NeutrophilsReceptor recyclingIntracellular vesicleTemporal cortexBlood collectionHealthy volunteersCX3CR1SerotonergicCentral nervous systemMetalloproteinaseSecretory pathwayPosterior parietal cortexInterleukin 8Enzyme-linked receptorAdhesionBiologyNeuroinflammationImmune systemC-C chemokine receptor type 6Cell biologyPharmacologyRespiratory burst
Publications 8
#1Stefan Düsterhöft (RWTH Aachen University)H-Index: 11
#2Selcan Kahveci-Türköz (RWTH Aachen University)
Last. Andreas Ludwig (RWTH Aachen University)H-Index: 35
view all 14 authors...
Membrane-tethered signalling proteins such as TNFα and many EGF receptor ligands undergo shedding by the metalloproteinase ADAM17 to get released. The pseudoproteases iRhom1 and iRhom2 are important for the transport, maturation and activity of ADAM17. Yet, the structural and functional requirements to promote the transport of the iRhom-ADAM17 complex have not yet been thoroughly investigated. Utilising in silico and in vitro methods, we here map the conserved iRhom homology domain (IRHD) and pr...
#1Anke Seifert (RWTH Aachen University)H-Index: 3
#2Stefan Düsterhöft (RWTH Aachen University)H-Index: 11
Last. Andreas Ludwig (RWTH Aachen University)H-Index: 63
view all 10 authors...
The metalloproteinase ADAM10 critically contributes to development, inflammation, and cancer and can be controlled by endogenous or synthetic inhibitors. Here, we demonstrate for the first time that loss of proteolytic activity of ADAM10 by either inhibition or loss of function mutations induces removal of the protease from the cell surface and the whole cell. This process is temperature dependent, restricted to mature ADAM10, and associated with an increased internalization, lysosomal degradati...
3 CitationsSource
#1Natalie Gasterich (RWTH Aachen University)H-Index: 1
#2Sophie Wetz (RWTH Aachen University)
Last. Tim Clarner (RWTH Aachen University)H-Index: 26
view all 12 authors...
The central nervous system (CNS) responds to diverse neurologic injuries with a vigorous activation of astrocytes. In addition to their role in the maintenance of CNS homeostasis and neuronal function, astrocytes are thought to participate in the regulation of innate and adaptive immune responses in the CNS. Following antigen recognition, reactive astrocytes may participate in the initiation of innate immune responses, and modulate adaptive immune response leading to the recruitment of periphera...
2 CitationsSource
#1Julia Krabbe (RWTH Aachen University)H-Index: 4
#2Viktor Beilmann (RWTH Aachen University)H-Index: 2
Last. Christian Martin (RWTH Aachen University)H-Index: 20
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In the isolation of polymorphonuclear neutrophils (PMNs) the technique and other external factors can have great influence on the quality and quantity of isolated neutrophils. To elucidate the influence of the blood collection technique, anticoagulants and storing temperature on isolated PMNs healthy volunteers provided blood samples with different needles and collection techniques, anticoagulants (EDTA, heparin, citrate) and storing temperatures (4, 22, 37 °C). From each blood sample PMNs were ...
1 CitationsSource
#1Anke Seifert (RWTH Aachen University)H-Index: 3
#2Justyna Wozniak (RWTH Aachen University)H-Index: 3
Last. Andreas Ludwig (RWTH Aachen University)H-Index: 63
view all 9 authors...
Uptake of bacteria by phagocytes is a crucial step in innate immune defence. Members of the disintegrin and metalloproteinase (ADAM) family critically control the immune response by limited proteolysis of surface expressed mediator molecules. Here, we investigated the significance of ADAM17 and its regulatory adapter molecule iRhom2 for bacterial uptake by phagocytes. Inhibition of metalloproteinase activity led to increased phagocytosis of pHrodo labelled Gram-negative and -positive bacteria (E...
2 CitationsSource
#1Daniela Dreymueller (RWTH Aachen University)H-Index: 16
#2Jessica Pruessmeyer (RWTH Aachen University)H-Index: 14
Last. Andreas Ludwig (RWTH Aachen University)H-Index: 63
view all 9 authors...
Alveolar leukocyte recruitment is a hallmark of acute lung inflammation and involves transmigration of leukocytes through endothelial and epithelial layers. The disintegrin and metalloproteinase (ADAM) 8 is expressed on human isolated leukocytic cells and can be further upregulated on cultured endothelial and epithelial cells by proinflammatory cytokines. By shRNA mediated knockdown we show that leukocytic ADAM8 is required on monocytic THP-1 cells for chemokine-induced chemotaxis as well as tra...
14 CitationsSource
#1Andrea Koenen (RWTH Aachen University)H-Index: 2
#2Aaron Babendreyer (RWTH Aachen University)H-Index: 8
Last. Daniela Dreymueller (RWTH Aachen University)H-Index: 16
view all 9 authors...
The CXC-chemokine receptor 6 (CXCR6) is a class A GTP-binding protein-coupled receptor (GPCRs) that mediates adhesion of leukocytes by interacting with the transmembrane cell surface-expressed chemokine ligand 16 (CXCL16), and also regulates leukocyte migration by interacting with the soluble shed variant of CXCL16. In contrast to virtually all other chemokine receptors with chemotactic activity, CXCR6 carries a DRF motif instead of the typical DRY motif as a key element in receptor activation a...
12 CitationsSource
#1Barbara Drueke (RWTH Aachen University)H-Index: 9
#2Sonja M.A. Schlaegel (RWTH Aachen University)H-Index: 2
Last. Maren Boecker (RWTH Aachen University)H-Index: 17
view all 7 authors...
Abstract In animal and human research, the neurotransmitter serotonin (5-HT) has been implicated in inhibitory control. Using functional magnetic resonance imaging (fMRI), the present study investigated the acute effects of pharmacological modulation of the serotonergic system on brain activation during response inhibition and re-engagement in healthy human volunteers. In a randomized double-blind placebo-controlled cross-over design 14 men received either a single oral dose of the selective ser...
17 CitationsSource