Jessica E. Fitzgerald
Northeastern University
AnalytePathologyFourier transform infrared spectroscopyNanotechnologyChemistryMaterials scienceIn vivoElectronic noseLewis lung carcinomaLiquid biopsyMedical diagnosticMouse xenograftEarly diseaseRaman spectroscopyComputer scienceSubstrate (electronics)PolymerSiliconFlow cytometryCirculating tumor cellDynamics (mechanics)Sensor arrayMedicineFluorescence
14Publications
4H-index
82Citations
Publications 14
Newest
#1Amber L. Williams (NU: Northeastern University)H-Index: 1
#1Amber Williams (NU: Northeastern University)H-Index: 3
Last. Mark Niedre (NU: Northeastern University)H-Index: 19
view all 5 authors...
Motivation Circulating tumor cells (CTCs) are widely studied using liquid biopsy methods that analyze fractionally-small peripheral blood (PB) samples. However, little is known about natural fluctuations in CTC numbers that may occur over short timescales in vivo, and how these may affect detection and enumeration of rare CTCs from small blood samples. Methods We recently developed an optical instrument called "diffuse in vivo flow cytometry" (DiFC) that uniquely allows continuous, non-invasive ...
1 CitationsSource
#1Jessica E. Fitzgerald (NU: Northeastern University)H-Index: 4
#2Brook K. Byrd (Dartmouth College)H-Index: 4
Last. Mark Niedre (NU: Northeastern University)H-Index: 19
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Subcutaneous (s.c.) tumor models are widely used in pre-clinical cancer metastasis research. Despite this, the dynamics and natural progression of circulating tumor cells (CTCs) and CTC clusters (CTCCs) in peripheral blood are poorly understood in these models. In this work, we used a new technique called ‘diffuse in vivo flow cytometry’ (DiFC) to study CTC and CTCC dissemination in an s.c. Lewis lung carcinoma (LLC) model in mice. Tumors were grown in the rear flank and we performed DiFC up to ...
3 CitationsSource
#1Peter Bartosik (NU: Northeastern University)H-Index: 3
#2Jessica E. Fitzgerald (NU: Northeastern University)H-Index: 4
Last. Mark Niedre (NU: Northeastern University)H-Index: 19
view all 6 authors...
Purpose: We recently developed a new fluorescence-based technique called "diffuse in vivo flow cytometry" (DiFC) for enumerating rare circulating tumor cells (CTCs) directly in the bloodstream. Non-specific tissue autofluorescence is a persistent problem, as it creates a background which may obscure signals from weakly-labeled CTCs. Here we investigated the use of upconverting nanoparticles (UCNPs) as a contrast agent for DiFC, which in principle could significantly reduce the autofluorescence b...
1 CitationsSource
#1Amber L. WilliamsH-Index: 1
#1Amber WilliamsH-Index: 3
Last. Mark NiedreH-Index: 19
view all 3 authors...
Source
#1Amber L. Williams (NU: Northeastern University)H-Index: 1
#1Amber Williams (NU: Northeastern University)H-Index: 3
Last. Mark Niedre (NU: Northeastern University)H-Index: 19
view all 3 authors...
Motivation: Circulating tumor cells (CTCs) are important in hematogenous cancer metastasis, and are usually studied by isolation from small peripheral blood samples. However, little is known about the short-term dynamics of CTC in vivo. Methods: We recently developed an instrument called 9diffuse in vivo flow cytometry9 (DiFC), that allows counting of fluorescently-labeled circulating cells without drawing blood samples. DiFC samples 50 μL of circulating peripheral blood per minute, allowing con...
3 CitationsSource
#1Jessica E. Fitzgerald (NU: Northeastern University)H-Index: 4
#2Jianliang Shen (WMU: Wenzhou Medical College)H-Index: 1
Last. Hicham Fenniri (NU: Northeastern University)H-Index: 36
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The development of cross-reactive sensor arrays for volatile organics (electronic noses, e-noses) is an active area of research. In this manuscript, we present a new format for barcoded polymer sensor arrays based on porous polymer beads. An array of nine self-encoded polymers was analyzed by Raman spectroscopy before and after exposure to a series of volatile organic compounds, and the changes in the vibrational fingerprints of their polymers was recorded before and after exposure. Our results ...
3 CitationsSource
#1Jessica E. Fitzgerald (NU: Northeastern University)H-Index: 4
#2Saber Shokat Fadaee (NU: Northeastern University)H-Index: 4
Last. Hicham Fenniri (NU: Northeastern University)H-Index: 36
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Abstract Cross-reactive sensor arrays have recently emerged as a powerful tool in medical diagnostics. Here we present a sensor array based on inexpensive, spectroscopically self-encoded polymers. The array was analyzed by Raman spectroscopy before and after exposure to a series of analyte solutions. The changes in the polymers’ vibrational fingerprints were further quantified using Pearson’s correlation coefficient between spectra before and after exposure. Multivariate data analysis, including...
3 CitationsSource
#1Mark NiedreH-Index: 19
Last. Ross BerbecoH-Index: 40
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#1Icell M. Sharafeldin (The: American University in Cairo)H-Index: 5
#2Jessica E. Fitzgerald (NU: Northeastern University)H-Index: 4
Last. Nageh K. Allam (The: American University in Cairo)H-Index: 36
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: Computational modeling has become an important tool for scientists to both predict the properties of materials and systems and to gain a better understanding of the underlying mechanisms. This chapter is a brief yet holistic introduction to computational modeling, focusing on density functional theoretical (DFT) methods. The different types of computational modeling methods, including molecular mechanics, semiempirical, and ab initio methods, as well as the different software available for com...
1 CitationsSource
#1Jessica E. Fitzgerald (NU: Northeastern University)H-Index: 4
#2Hicham Fenniri (NU: Northeastern University)H-Index: 36
The development of chemical sensors continues to be an active area of research, especially the development of a practical electronic nose. Here, we present a spectroscopic chemical sensor based on an array of 64 self-encoded polymer films deposited on a microfabricated silicon substrate. The polymer arrays were analyzed by FTIR and Raman spectroscopy before and after exposure to a series of organic volatiles to monitor changes in their vibrational fingerprints. We show here that the spectroscopi...
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