Kai-Chih Huang
Boston University
OpticsBiophysicsMicroscopyHyperspectral imagingLine (formation)Fluorescence microscopeRaman scatteringFiber laserSpectral signatureChemistryMaterials scienceImage resolutionLipid dropletCytometryMultiplexMicrosecondRaman spectroscopyOptoelectronicsChemical imagingMicroscopeFluorescence
27Publications
9H-index
219Citations
Publications 26
Newest
#1Minghua Zhuge (ZJU: Zhejiang University)H-Index: 1
#1Minghua Zhuge (ZJU: Zhejiang University)H-Index: 1
Last. Ji-Xin Cheng (BU: Boston University)H-Index: 83
view all 11 authors...
High-sensitivity chemical imaging offers a window to decipher the molecular orchestra inside a living system. Based on vibrational fingerprint signatures, coherent Raman scattering microscopy provides a label-free approach to map biomolecules and drug molecules inside a cell. Yet, by near-infrared (NIR) pulse excitation, the sensitivity is limited to millimolar concentration for endogenous biomolecules. Here, the imaging sensitivity of stimulated Raman scattering (SRS) is significantly boosted f...
2 CitationsSource
#1Hai Bi (Harvard University)H-Index: 15
Last. Semion K. SaikinH-Index: 26
view all 13 authors...
Crystal engineering is a practical approach for tailoring material properties. This approach has been widely studied for modulating optical and electrical properties of semiconductors. However, the properties of organic molecular crystals are difficult to control following a similar engineering route. In this Letter, we demonstrate that engineered crystals of Alq3 and Ir(ppy)3 complexes, which are commonly used in organic light-emitting technologies, possess intriguing functional properties. Spe...
Source
#1Jiayingzi Wu (BU: Boston University)H-Index: 2
#2Hyeon Jeong Lee (BU: Boston University)H-Index: 10
Last. Ji-Xin Cheng (BU: Boston University)H-Index: 83
view all 11 authors...
Development of molecular probes holds great promise for early diagnosis of aggressive prostate cancer. Here, 2-[3-(1,3-dicarboxypropyl) ureido] pentanedioic acid (DUPA)-conjugated ligand and bis-isoindigo-based polymer (BTII) are synthesized to formulate semiconducting polymer nanoparticles (BTII-DUPA SPN) as a prostate-specific membrane antigen (PSMA)-targeted probe for prostate cancer imaging in the NIR-II window. Insights into the interaction of the imaging probes with the biological targets ...
11 CitationsSource
#1Kai-Chih Huang (BU: Boston University)H-Index: 9
#2Junjie Li (BU: Boston University)H-Index: 16
Last. Ji-Xin Cheng (BU: Boston University)H-Index: 83
view all 5 authors...
Summary In situ measurement of cellular metabolites is still a challenge in biology. Conventional methods, such as mass spectrometry or fluorescence microscopy, would either destruct the sample or introduce strong perturbations to target molecules. Here, we present multiplex stimulated Raman scattering (SRS) imaging cytometry as a label-free single-cell analysis platform with chemical specificity and high-throughput capabilities. Using SRS imaging cytometry, we studied the metabolic responses of...
13 CitationsSource
#1Pu-Ting Dong (BU: Boston University)H-Index: 6
#2Yuewei Zhan (BU: Boston University)H-Index: 1
Last. Ji-Xin Cheng (BU: Boston University)H-Index: 83
view all 7 authors...
Candidemia remains the fourth most common cause of nosocomial bloodstream infections. For more than a half-century, amphotericin B (Amp B) has been the last line of defense in the treatment of life-threatening fungal infections. However, during the past several years, severe infections due to Amp B-resistant Candida spp. isolates have been increasingly reported. Here, through polarization stimulated Raman scattering microscopy, Amp B was found to accumulate largely in the cell membrane of Candid...
Source
#1Kai-Chih HuangH-Index: 9
#2Junjie LiH-Index: 16
Last. Ji-Xin Cheng (BU: Boston University)H-Index: 83
view all 5 authors...
In situ measurement of cellular metabolites is still a challenge in biology. Conventional methods, such as mass spectrometry or fluorescence microscopy, would either destruct the sample or introduce strong perturbations to the functions of target molecules. Here, we present multiplex stimulated Raman scattering (SRS) imaging cytometry as a label-free single-cell analysis platform with chemical specifity, and high-throughput capabilities. Cellular compartments such as lipid droplets, endoplasmic ...
#1Andy Chen (Purdue University)H-Index: 10
#1Andy J. Chen (Purdue University)H-Index: 3
Last. Ji-Xin Cheng (BU: Boston University)H-Index: 83
view all 9 authors...
Hemozoin, the heme detoxification end product in malaria parasites during their growth in the red blood cells (RBCs), serves as an important marker for diagnosis and treatment target of malaria disease. However, the current method for hemozoin-targeted drug screening mainly relies on in-vitro β-hematin inhibition assays, which may lead to false-positive events due to under-representation of the real hemozoin crystal. Quantitative in-situ imaging of hemozoin is highly desired for high-throughput ...
5 CitationsSource
#1Hyeon Jeong Lee (BU: Boston University)H-Index: 10
#2Kai-Chih Huang (BU: Boston University)H-Index: 9
Last. Ji-Xin ChengH-Index: 83
view all 8 authors...
Voltage imaging allows mapping of membrane potential in living cells. Yet, current intensity-based imaging approaches are limited to relative membrane potential changes, missing important information conveyed by the absolute value of membrane voltage. This challenge arises from various factors affecting the signal intensity, such as concentration, illumination intensity, and photobleaching. Here, we demonstrate electronic pre-resonance hyperspectral stimulated Raman scattering (EPR-hSRS) for spe...
2 CitationsSource
#1Haonan LinH-Index: 6
#2Fengyuan Deng (BU: Boston University)H-Index: 5
Last. Ji-Xin Cheng (BU: Boston University)H-Index: 83
view all 5 authors...
We present a stimulated Raman scattering imaging system which acquires a Raman spectrum within 20 µs. A U-Net deep learning network is applied to maintain the sensitivity at high speeds, enabling high-throughput label-free spectroscopic imaging of cells and tissues. © 2019 The Author(s)
1 CitationsSource
#1Pu-Ting Dong (BU: Boston University)H-Index: 6
#2Haonan Lin (BU: Boston University)H-Index: 6
Last. Ji-Xin ChengH-Index: 83
view all 4 authors...
As a stable and accurate biomarker, glycated hemoglobin (HbA1c) is clinically used to diagnose diabetes with a threshold of 6.5% among total hemoglobin (Hb). Current methods such as boronate affinity chromatography involve complex processing of large-volume blood samples. Moreover, these methods cannot measure HbA1c fraction at single–red blood cell (RBC) level, thus unable to separate the contribution from other factors such as RBC lifetime. Here, we demonstrate a spectroscopic transient absorp...
10 CitationsSource