Robert P. Mecham
Washington University in St. Louis
AnatomyBiophysicsInternal medicineEndocrinologyPathologyMolecular biologyChemistryExtracellular matrixMicrofibrilElastinAortaElastic fiberTropoelastinDesmosineElastic fiber assemblyBiochemistryMedicineFibrillinBiologyCell biology
Publications 351
#1Chien-Jung Lin (WashU: Washington University in St. Louis)H-Index: 1
#2Robert P. Mecham (WashU: Washington University in St. Louis)H-Index: 91
Abstract null null Smooth muscle cells (SMCs) are specialized cells present in many organs where they serve diverse tissue-specific functions. Using the Tabula Muris compendium of single-cell RNA sequencing data, we extracted individual SMC transcriptomes from eight mouse tissues to investigate the transcriptomic landscape of tissue-specific SMCs. We identified marker genes, signaling pathways, and biological processes enriched in tissue-specific SMCs, and inferred potential ligand-receptor inte...
#1Chien-Jung Lin (WashU: Washington University in St. Louis)H-Index: 1
#2Bridget M. Hunkins (WashU: Washington University in St. Louis)H-Index: 1
Last. Robert P. Mecham (WashU: Washington University in St. Louis)H-Index: 91
view all 6 authors...
Objective: Using a mouse model of Eln (elastin) insufficiency that spontaneously develops neointima in the ascending aorta, we sought to understand the origin and phenotypic heterogeneity of smooth...
#2Jeffrey T. Kroetsch (U of T: University of Toronto)H-Index: 9
#4Negin Khosraviani (U of T: University of Toronto)H-Index: 2
#1Sarah Carney (Cleveland Clinic)H-Index: 2
#2Thomas J. Broekelmann (WashU: Washington University in St. Louis)H-Index: 35
Last. Anand Ramamurthi (Lehigh University)H-Index: 24
view all 4 authors...
Elastic fibers do not naturally regenerate in many proteolytic disorders, such as in abdominal aortic aneurysms (AAAs), and prevent restoration of tissue homeostasis. We have shown drug-based attenuation of the stress-activated protein kinase, JNK-2 to stimulate elastic matrix neoassembly and to attenuate cellular proteolytic activity. We now investigate if JNK2 gene knockdown with siRNA provides greater specificity of action and improved regenerative/anti-proteolytic outcomes in a proteolytic i...
#1Robert B. Hinton (Cincinnati Children's Hospital Medical Center)H-Index: 27
#2Amy L. Juraszek (Boston Children's Hospital)H-Index: 17
Last. Kevin E. Bove (Cincinnati Children's Hospital Medical Center)H-Index: 37
view all 7 authors...
Elastic fiber fragmentation (EFF) is a hallmark of aortic valve disease (AVD), and neovascularization has been identified as a late finding related to inflammation. We sought to characterize the relationship between early EFF and aberrant angiogenesis. To examine disease progression, regional anatomy and pathology of aortic valve tissue were assessed using histochemistry, immunohistochemistry, and electron microscopy from early-onset (<40 yo) and late-onset (≥40 yo) non-syndromic AVD specimens. ...
#1William C. ParksH-Index: 88
#2Robert P. MechamH-Index: 91
The process of cellular differentiation is guided by molecular controls that regulate the expression of cell-specific genes. Clearly, these controls must be precise in both their specificity and temporal appearance. The signals that direct the molecular regulation of differentiation are intrinsic and extrinsic (extracellular) factors. Intrinsic mechanisms require a change in activity (e.g., by molecular modification) or concentration (by synthesis regulation or dilution due to cell division) of ...
#2Jared S. Elenbaas (WashU: Washington University in St. Louis)H-Index: 6
#3Arturo Alisio (WashU: Washington University in St. Louis)H-Index: 5
Last. Nathan O. Stitziel (WashU: Washington University in St. Louis)H-Index: 33
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A low-frequency variant of sushi, von Willebrand factor type A, EGF, and pentraxin domain-containing protein 1 (SVEP1), an extracellular matrix protein, is associated with risk of coronary disease in humans independent of plasma lipids. Despite a robust statistical association, if and how SVEP1 might contribute to atherosclerosis remained unclear. Here, using Mendelian randomization and complementary mouse models, we provide evidence that SVEP1 promotes atherosclerosis in humans and mice and is ...
#1Haixia Zhang (WashU: Washington University in St. Louis)H-Index: 19
#2Alex Hanson (WashU: Washington University in St. Louis)H-Index: 2
Last. Colin G. Nichols (WashU: Washington University in St. Louis)H-Index: 77
view all 16 authors...
Cantu Syndrome (CS) is caused by gain-of-function (GOF) mutations in pore-forming (Kir6.1, KCNJ8) and accessory (SUR2, ABCC9) KATP channel subunits, the most common mutations being SUR2[R1154Q] and SUR2[R1154W], carried by ~30% of patients. We used CRISPR/Cas9 genome engineering to introduce the equivalent of human SUR2[R1154Q] mutation to the mouse ABCC9 gene. Along with minimal CS disease features, R1154Q cardiomyocytes and vascular smooth muscle showed much lower KATP current density and pina...
1 CitationsSource
#1Angela Troia (NIH: National Institutes of Health)H-Index: 2
#2Russell H Knutsen (NIH: National Institutes of Health)H-Index: 1
Last. Beth A. Kozel (NIH: National Institutes of Health)H-Index: 20
view all 11 authors...
3 CitationsSource