Annemarie K. Leonard
University of Notre Dame
CancerInternal medicineDownregulation and upregulationPathologyOncologyGene expressionLive cell imagingMasson's trichrome stainAdipose tissueGene knockdownZymographyExtracellular matrixIn vitroImmunologyImmunohistochemistryIn vivoEx vivoPeritoneumFemale reproductive systemInterferonTumor-infiltrating lymphocytesPeritoneal cavityMetastasisUterusDiseaseContext (language use)Ovarian cancerPeritoneal NeoplasmGynecologyDisease progressionIn situ zymographyVisualizationProtein structureCancer researchConnective tissueMedicineCell cultureBiologyImmune systemCell biology
7Publications
2H-index
20Citations
Publications 7
Newest
#1Yueying Liu (ND: University of Notre Dame)H-Index: 16
#2Jing Yang (ND: University of Notre Dame)H-Index: 6
Last. M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
view all 28 authors...
Abstract The majority of women with ovarian cancer are diagnosed with metastatic disease, therefore elucidating molecular events that contribute to successful metastatic dissemination may identify additional targets for therapeutic intervention and thereby positively impact survival. Using two human high grade serous ovarian cancer cell lines with inactive TP53 and multiple rounds of serial in vivo passaging, we generated sublines with significantly accelerated intra-peritoneal (IP) growth. Comp...
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#1Elizabeth I. Harper (ND: University of Notre Dame)H-Index: 6
#2Elizabeth Loughran (ND: University of Notre Dame)H-Index: 7
Last. M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
view all 14 authors...
Ovarian cancer (OvCa) is the deadliest cancer of the female reproductive system, ranking fifth in overall cancer deaths among women. Unlike the majority of cancers, OvCa metastasizes via diffusion through the peritoneal cavity, resulting in multiple metastatic sites, including the omentum and peritoneum. These metastasizing OvCa cells induce rapid mesothelial cell retraction and readily adhere to the sub-mesothelial collagen of the extracellular matrix. Epidemiologic data identifies age as a sig...
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#1Elizabeth Loughran (ND: University of Notre Dame)H-Index: 7
#2Annemarie K. Leonard (ND: University of Notre Dame)H-Index: 2
Last. M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
view all 17 authors...
Abstract Ovarian cancer, the most deadly gynecological malignancy in U.S. women, metastasizes uniquely, spreading through the peritoneal cavity and often generating widespread metastatic sites before diagnosis. The vast majority of ovarian cancer cases occur in women over 40 and the median age at diagnosis is 63. Additionally, elderly women receive poorer prognoses when diagnosed with ovarian cancer. Despite age being a significant risk factor for the development of this cancer, there are little...
9 CitationsSource
#1Annemarie K. Leonard (ND: University of Notre Dame)H-Index: 2
#2Elizabeth Loughran (ND: University of Notre Dame)H-Index: 7
Last. M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
view all 9 authors...
Abstract This chapter highlights methods for visualization and analysis of extracellular matrix (ECM) proteins, with particular emphasis on collagen type I, the most abundant protein in mammals. Protocols described range from advanced imaging of complex in vivo matrices to simple biochemical analysis of individual ECM proteins. The first section of this chapter describes common methods to image ECM components and includes protocols for second harmonic generation, scanning electron microscopy, an...
10 CitationsSource
#1Elizabeth Loughran (ND: University of Notre Dame)H-Index: 7
#2Ryan C. Phan (ND: University of Notre Dame)H-Index: 1
Last. M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
view all 15 authors...
Ovarian cancer is the fifth leading cause of cancer deaths in U.S. women and the deadliest gynecologic malignancy. This lethality is largely due to the fact that most cases are diagnosed at metastatic stages of the disease when the prognosis is poor. Epidemiologic studies consistently demonstrate that parous women have a reduced risk of developing ovarian cancer, with a greater number of births affording greater protection; however little is known about the impact of parity on ovarian cancer met...
1 CitationsSource
Ovarian cancer (OvCa) is the leading gynecological malignancy in women in the United States. OvCa metastasizes uniquely, spreading through the peritoneal cavity and generating widespread metastatic sites. The vast majority of OvCa cases occur in women over 40 and the median age at diagnosis is 63 (SEER). Despite age being a significant risk factor for the development of OvCa, there is a paucity of studies addressing the role of aging in OvCa metastasis. To our knowledge, there are no reports uti...
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#1Elizabeth Loughran (ND: University of Notre Dame)H-Index: 7
#2Ryan Phan (ND: University of Notre Dame)
Last. M. Sharon Stack (ND: University of Notre Dame)H-Index: 17
view all 11 authors...
Ovarian cancer is the most fatal gynecological cancer. Epithelial ovarian cancer (OvCa), the most common subtype, usually goes undetected until metastatic and often fatal stages of the disease. OvCa follows a unique form of metastasis, spreading through the peritoneal cavity and forming metastatic sites on the peritoneum. Epidemiologic data suggest that child-bearing, or parity, reduces a woman9s risk of developing ovarian cancer, with more births providing greater protection. Despite the associ...
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