Hongyan Ding
Anhui Agricultural University
PhosphorylationInternal medicineDownregulation and upregulationEndocrinologyProinflammatory cytokineKinaseChemistryPeroxisome proliferator-activated receptorApoptosisOxidative stressHepatocyteAMP-activated protein kinaseInflammationNitric oxide synthaseFatty liverAMPKPyrrolidine dithiocarbamateLipid oxidationLipid metabolismFatty acid synthasep38 mitogen-activated protein kinasesBiochemistryMAPK/ERK pathwayOxidative phosphorylationSignal transductionVery low-density lipoproteinBiologyHedgehog signaling pathwayCell biologyProtein kinase A
18Publications
7H-index
249Citations
Publications 17
Newest
#1Hongyan Ding (AAU: Anhui Agricultural University)H-Index: 7
Sirtuin 1 (SIRT1), an NAD-dependent protein deacetylase, plays a central role in the control of lipid metabolism in nonruminants. However, the role of SIRT1 in hepatic lipid metabolism in dairy cows with fatty liver is not well known. Thus, we used isolated primary bovine hepatocytes to determine the role of SIRT1 in protecting cells against oleic acid (OA)-induced steatosis. Recombinant adenoviruses to overexpress (AD-GFP-SIRT1-E) or knockdown (AD-GFP-SIRT1-N) SIRT1 were used for transduction o...
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#1Hongyan Ding (AAU: Anhui Agricultural University)H-Index: 7
#2Yu Li (AAU: Anhui Agricultural University)H-Index: 11
Last. Jinjie Wu (AAU: Anhui Agricultural University)H-Index: 10
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Epigallocatechin-3-gallate (EGCG) plays a crucial role in hepatic lipid metabolism. However, the underlying regulatory mechanism of hepatic lipid metabolism by EGCG in canine is unclear. Primary canine hepatocytes were treated with EGCG (0.01, 0.1, or 1 μM) and BML-275 (an AMP-activated protein kinase [AMPK] inhibitor) to study the effects of EGCG on the gene and protein expressions associated with AMPK signaling pathway. Data showed that treatment with EGCG had greater activation of AMPK, as we...
1 CitationsSource
#1Shibin Feng (AAU: Anhui Agricultural University)H-Index: 10
#2Hongyan Ding (AAU: Anhui Agricultural University)H-Index: 7
Last. Jinjie Wu (AAU: Anhui Agricultural University)H-Index: 10
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The aim of the present study was to investigate the immunoregulatory effects of Astragalus polysaccharide (APS) on RAW264.7 cells. The production of cytokines by RAW264.7 cells was analyzed using ELISA, while cell viability and optimal concentration of APS were assessed using the Cell Counting Kit-8 assay. In addition, the mRNA levels of IL-6, inducible nitric oxide synthase (iNOS) and TNF-α were determined by reverse transcription-quantitative PCR analysis. The levels of co-stimulatory molecule...
1 CitationsSource
Elevated plasma nonesterified fatty acid (NEFA) levels and hepatocytes damage are characteristics of ketosis in dairy cows. Oxidative stress is associated with the pathogenesis of NEFA-induced liver damage. However, the exact mechanism by which oxidative stress mediates NEFA-induced hepatocytes apoptosis and liver injury remains poorly understood. The results of the present study demonstrated that NEFA contribute to reactive oxygen species (ROS) generation, resulting in an imbalance between oxid...
11 CitationsSource
#1Yu Li (JLU: Jilin University)H-Index: 11
: The ketotic cows displayed hepatic lipid metabolic disorder and high blood concentration of glucagon. Importantly, adenosine monophosphate-activated protein kinase (AMPK) signaling pathway plays an important role in the hepatic lipid homeostasis. Therefore, the aim of this study was to investigate the effect of glucagon on AMPK pathway and its underlying mechanism on lipid metabolism in cow hepatocytes. Cow hepatocytes were cultured and treated with glucagon and AMPK inhibitor (BML-275). The r...
6 CitationsSource
#1Siyi Su (AAU: Anhui Agricultural University)H-Index: 1
#2Xiaoyu Li (AAU: Anhui Agricultural University)H-Index: 2
Last. Jinjie Wu (AAU: Anhui Agricultural University)H-Index: 10
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Rutin, found widely in traditional Chinese medicine materials, is used to treat eye swelling and pain, hypertension, and hyperlipidemia. In the present study, a mouse mastitis model induced by lipopolysaccharide (LPS) was established to explore rutin’s inhibitory mechanism on mastitis via nuclear factor kappa B (NF-κB) inflammatory signaling and the relationship between NF-κB signaling and endoplasmic reticulum (ER) stress. Mice were divided into six groups: Control group, LPS model group, LPS +...
12 CitationsSource
#2Yu Li (AAU: Anhui Agricultural University)H-Index: 11
Dairy cows with type II ketosis display hepatic fat accumulation and hyperinsulinemia, but the underlying mechanism is not completely clear. This study aimed to clarify the regulation of lipid metabolism by insulin in cow hepatocytes. In vitro, cow hepatocytes were treated with 0, 1, 10, or 100 n m insulin in the presence or absence of AICAR (an AMP-activated protein kinase alpha (AMPKα) activator). The results showed that insulin decreased AMPKα phosphorylation. This inactivation of AMPKα incre...
8 CitationsSource
#1Yu Li (AAU: Anhui Agricultural University)H-Index: 11
#2Ning Hao (AAU: Anhui Agricultural University)H-Index: 2
Last. Jinjie Wu (AAU: Anhui Agricultural University)H-Index: 10
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The current study aimed at investigating the effects of flavonoids from Astragalus complanatus (FAC) on the proliferation, the contents, and gene expression levels of cytokines, secretion of surface stimulating factors, cell cycle, and the expression level of the NF-κB signalling pathway in RAW264.7 cells. Our results revealed that compared with control group, the contents of IL-6, IL-1β, TNF-α, and NO and the mRNA expression levels of IL-6, IL-1β, TNF-α, and iNOS in FAC-treated groups significa...
6 CitationsSource
#1Yu LiH-Index: 11
#2Ning HaoH-Index: 2
Last. Jinjie WuH-Index: 10
view all 12 authors...
#1Yu Li (AAU: Anhui Agricultural University)H-Index: 11
#2Tingting Meng (AAU: Anhui Agricultural University)H-Index: 3
Last. Jinjie Wu (AAU: Anhui Agricultural University)H-Index: 10
view all 13 authors...
Abstract The present study was designed to investigate the effects of Astragaloside IV (ASIV) on the immune functions of RAW264.7 cells. Compared with control group, the concentrations of interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α) and nitric oxide (NO) were higher in the 100 μg/mL ASIV-treatment group. The interleukin 6 (IL-6) concentration was significantly higher in the 50 and 100 μg/mL ASIV-treatment groups. The relative mRNA expression levels of IL-1β, TNF-α and inducible nitric...
23 CitationsSource