Di Huang
Nankai University
FermentationMetabolic networkGeneComposite numberComposite materialChemistryMaterials scienceMetabolomicsScanning electron microscopeStreptomyces tsukubaensisFumaric acidCoatingChemical engineeringBiosynthesisBiochemistryVirulenceMetabolismMetabolic engineeringPorosityBiology
53Publications
21H-index
1,415Citations
Publications 53
Newest
#1Bingling Zhang (NKU: Nankai University)H-Index: 1
#2Linbo Sun (NKU: Nankai University)H-Index: 2
Last. Wei Wang (NKU: Nankai University)H-Index: 18
view all 7 authors...
1 CitationsSource
#1Xiaolong Jiang (NKU: Nankai University)H-Index: 3
#2Jing Bai (NKU: Nankai University)H-Index: 1
Last. Lu Feng (NKU: Nankai University)H-Index: 35
view all 11 authors...
Abstract Extraintestinal pathogenic Escherichia coli (ExPEC) has presented a major clinical infection emerged in the past decades. O-polysaccharide (OPS)-based glycoconjugate vaccines produced using the bacterial glycosylation machinery can be utilized to confer protection against such infection. However, constructing a low-cost microbial cell factory for high-efficient production of OPS-based glycoconjugate vaccines remains challenging. Here, we engineered a glyco-optimized chassis strain by re...
1 CitationsSource
#1Jian YuanH-Index: 3
#2Xiaolong JiangH-Index: 3
Last. Di Huang
view all 11 authors...
Galectin-3 (Gal-3), a member of the β-galactoside-binding lectin family, involved in tumor angiogenesis and metastasis, is considered a diagnostic biomarker and a promising treatment target for early cancer. Gal-3 recognizes the poly-N-acetyllactosamine (poly-LacNAc)-based carbohydrate motifs of glycoproteins and glycolipids. To date, protein glycosylation with poly-LacNAc has been mainly achieved by chemical or chemoenzymatic strategies. In this study, a green artificial biosynthesis pathway fo...
Source
#1Lingyan Jiang (NKU: Nankai University)H-Index: 5
#2Peisheng Wang (NKU: Nankai University)H-Index: 3
Last. Lei Wang (NKU: Nankai University)H-Index: 56
view all 17 authors...
Salmonella Typhimurium establishes systemic infection by replicating in host macrophages. Here we show that macrophages infected with S. Typhimurium exhibit upregulated glycolysis and decreased serine synthesis, leading to accumulation of glycolytic intermediates. The effects on serine synthesis are mediated by bacterial protein SopE2, a type III secretion system (T3SS) effector encoded in pathogenicity island SPI-1. The changes in host metabolism promote intracellular replication of S. Typhimur...
2 CitationsSource
#1Huanhuan Liu (Tianjin University of Science and Technology)H-Index: 4
#2Jing Zhang (Tianjin University of Science and Technology)H-Index: 1
Last. Di Huang (NKU: Nankai University)H-Index: 21
view all 11 authors...
1 CitationsSource
#1Xiaorui SongH-Index: 2
#2Huan ZhangH-Index: 2
Last. Lingyan Jiang (NKU: Nankai University)H-Index: 5
view all 9 authors...
Salmonella enterica serovar Typhimurium (S. Typhimurium) is an important gram-negative intracellular pathogen that infects humans and animals. More than 50 putative regulatory proteins have been identified in the S. Typhimurium genome, but few have been clearly defined. In this study, the physiological function and regulatory role of STM14_3563, which encodes a ParD family putative transcriptional regulator in S. Typhimurium, were investigated. Macrophage replication assays and mice experiments ...
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#1Lingyan Jiang (NKU: Nankai University)H-Index: 5
#2Peisheng Wang (NKU: Nankai University)H-Index: 3
Last. Bin Liu (NKU: Nankai University)H-Index: 119
view all 9 authors...
To establish systemic infections, Salmonella enterica serovar Typhimurium (S. Typhimurium) requires Salmonella pathogenicity island 2 (SPI-2) to survive and replicate within macrophages. High expression of many SPI-2 genes during the entire intracellular growth period within macrophages is essential, as it contributes to the formation of Salmonella-containing vacuole and bacterial replication. However, the regulatory mechanisms underlying the sustained induction of SPI-2 within macrophages are n...
4 CitationsSource
#1Cheng Wang (NWAFU: Northwest A&F University)H-Index: 1
#2Junhua Wang (CAS: Chinese Academy of Sciences)H-Index: 1
Last. Di Huang (NKU: Nankai University)H-Index: 21
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: Ascomycin (FK520) is a macrocyclic antibiotic that also exhibits antifungal and immunosuppressive activity. However, its relatively low titer and yield have hampered commercial application. Here, we have successfully constructed an efficient ascomycin-producing strain of Streptomyces hygroscopicus with high titer and yield, using a novel combinatorial engineering approach based on the identification of targets involved in both metabolic and transcriptional regulation. First, we investigated th...
7 CitationsSource
#1Dalong Hu (USYD: University of Sydney)H-Index: 3
#2Zhiqiu Yin (NKU: Nankai University)H-Index: 3
Last. Bin Liu (NKU: Nankai University)H-Index: 119
view all 15 authors...
ABSTRACT The 7th cholera pandemic began in 1961 in Sulawesi, Indonesia, and then spread around the world in at least three waves. However, the lack of genome sequences for Vibrio cholerae strains under long-term surveillance in East Asia, especially in China, has restricted our understanding of the dynamics of the intracountry and intercountry evolution and transmission of the 7th-pandemic clones. In this study, we obtained the genome sequences of 60 V. cholerae strains isolated in Shanghai, the...
2 CitationsSource
#1Xiaorui SongH-Index: 2
#2Huan ZhangH-Index: 2
Last. Lingyan Jiang (NKU: Nankai University)H-Index: 5
view all 10 authors...
: Aim: Determination of the virulence regulatory network controlled by the ATP-dependent Lon protease in Salmonella enterica serovar Typhimurium. Materials & methods: The effect of Lon on S. Typhimurium virulence genes expression was investigated by RNA sequencing, and virulence-associated phenotypes between the wild-type and lon mutant were compared. Results:SPI-1, SPI-4, SPI-9 and flagellar genes were activated, while SPI-2 genes were repressed in the lon mutant. Accordingly, the lon mutant ex...
2 CitationsSource