Sylvie Dunoyer-Geindre
University of Geneva
PromoterHaematopoiesisGene silencingEndothelial stem cellInternal medicineEndocrinologyPathologymicroRNAGene expressionMolecular biologyAntibodyCell adhesion moleculeReceptorTumor necrosis factor alphaChemistryTissue factorDNA methylationProgenitor cellImmunologyEndotheliumUmbilical veinFluvastatinPlasminogen activatorLeukocyte adhesion moleculeEpigeneticsTransfectionMedicineBiologyPlateletCell biologyPharmacology
33Publications
16H-index
875Citations
Publications 33
Newest
#1Alix Garcia (University of Geneva)H-Index: 3
#2Sylvie Dunoyer-Geindre (University of Geneva)H-Index: 16
Last. Pierre Fontana (University of Geneva)H-Index: 31
view all 3 authors...
MicroRNAs (miRNAs) are a class of non-coding RNAs known to repress mRNA translation and subsequent protein production. miRNAs are predicted to modulate many targets and are involved in regulating various cellular processes. Identifying their role in cell function regulation may allow circulating miRNAs to be used as diagnostic or prognostic markers of various diseases. Increasing numbers of clinical studies have shown associations between circulating miRNA levels and platelet reactivity or the r...
1 CitationsSource
#1Alix Garcia (University of Geneva)H-Index: 3
#2Sylvie Dunoyer-Geindre (University of Geneva)H-Index: 16
Last. Pierre Fontana (University of Geneva)H-Index: 31
view all 6 authors...
BACKGROUND Several platelet-derived miRNAs are associated with platelet reactivity (PR) and clinical outcome in cardiovascular patients. We previously showed an association between miR-204-5p and PR in stable cardiovascular patients, but data on functional mechanisms are lacking. AIMS To validate miR-204-5p as a regulator of PR in platelet-like structures (PLS) derived from human megakaryocytes and to address mechanistic issues. METHODS Human hematopoietic stem cells were differentiated into meg...
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Platelet reactivity (PR), a key pharmacodynamic (PD) component of the action of antiplatelet drugs in cardiovascular disease (CVD) patients, is highly variable. PR is associated with occurrence or recurrence of thrombotic and bleeding events, but this association is modulated by several factors. Conventional pharmacogenetics explains a minor part of this PR variability, and among determinants of PR, circulating microRNAs (miRNAs) have been the focus of attention during these last years as biomar...
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#1Alix Garcia (University of Geneva)H-Index: 3
#2Sylvie Dunoyer-Geindre (University of Geneva)H-Index: 16
Last. Pierre Fontana (Geneva College)H-Index: 31
view all 6 authors...
MicroRNAs (miRNAs) are small noncoding RNAs modulating protein production. They are key players in regulation of cell function and are considered as biomarkers in several diseases. The identification of the proteins they regulate, and their impact on cell physiology, may delineate their role as diagnostic or prognostic markers and identify new therapeutic strategies. During the last 3 decades, development of a large panel of techniques has given rise to multiple models dedicated to the study of ...
5 CitationsSource
#1Veronika Zapilko (University of Geneva)H-Index: 2
#2Richard J. Fish (University of Geneva)H-Index: 20
Last. Pierre Fontana (Geneva College)H-Index: 31
view all 8 authors...
Circulating microRNA (miRNA) expression profiles correlate with platelet reactivity. MiR-126 is a promising candidates in this regard. We generated a transgenic zebrafish line with thrombocyte-spec...
4 CitationsSource
#1Alix Garcia (University of Geneva)H-Index: 3
#2Sylvie Dunoyer-Geindre (University of Geneva)H-Index: 16
Last. Pierre Fontana (Geneva College)H-Index: 31
view all 6 authors...
Background Platelets are an abundant source of micro-ribonucleic acids (miRNAs) that may play a role in the regulation of platelet function. Some miRNAs, such as miR-126-3p, have been noted as potential biomarkers of platelet reactivity and the recurrence of cardiovascular events. However, the biological relevance of these associations remains uncertain, and the functional validation of candidate miRNAs on human-derived cells is lacking. Objective This article functionally validates miR-126-3p a...
19 CitationsSource
#1Alix Garcia (University of Geneva)H-Index: 3
#2Sylvie Dunoyer-Geindre (University of Geneva)H-Index: 16
Last. Pierre Fontana (Geneva College)H-Index: 31
view all 6 authors...
Background Platelets are an abundant source of microRNAs (miRNAs), which may play a role in the regulation of platelet function. Some miRNAs, such as miR-126-3p, are pointed out as potential biomarkers of platelet reactivity and recurrence of cardiovascular events. However, the biological relevance of these associations remains uncertain and functional validation of these candidate miRNAs on human-derived cells is lacking. Purpose To functionally validate miR-126-3p as regulator of platelet reac...
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The characteristic hemorrhages of acute promyelocytic leukemia (APL) are caused in part by the high expression of tissue factor (TF) on leukemic cells, which also produce TNF and IL-1β, proinflammatory cytokines known to increase TF in various cell types. Exposure of NB4 cells, an APL cell line, to all-trans retinoic acid (ATRA) or arsenic trioxide (ATO) rapidly and strongly reduced TF mRNA. Both drugs also reduced TNF mRNA, but later, and moreover increased IL-1β mRNA. The effect on procoagulan...
8 CitationsSource
#1Martina Barnevik Olsson (University of Gothenburg)H-Index: 8
#2Karin Hultman (Lund University)H-Index: 11
Last. Christina Jern (University of Gothenburg)H-Index: 43
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The serine protease tissue-type plasminogen activator (t-PA) is involved in both vital physiological brain processes, such as synaptic plasticity, and pathophysiological conditions, such as neurodegeneration and ischemic stroke. Recent data suggest that epigenetic mechanisms play an important role in the regulation of t-PA in human endothelial cells. However, there are limited data on epigenetic regulation of t-PA in human brain-derived cells. We demonstrate that treatment of cultured human neur...
2 CitationsSource
#1Sylvie Dunoyer-Geindre (University of Geneva)H-Index: 16
#2Anne-Sophie Rivier-Cordey (University of Geneva)H-Index: 4
Last. Egbert K. O. Kruithof (University of Geneva)H-Index: 41
view all 5 authors...
: Expression of the tissue-type plasminogen activator gene (t-PA; gene name PLAT) is regulated, in part, by epigenetic mechanisms. We investigated the relationship between PLAT methylation and PLAT expression in five primary human cell types and six transformed cell lines. CpG methylation was analyzed in the proximal PLAT gene promoter and near the multihormone responsive enhancer (MHRE) -7.3 kilobase pairs upstream of the PLAT transcriptional start site (TSS, -7.3 kb). In Bowes melanoma cells, ...
6 CitationsSource