Shaojun Yang
Rutgers University
ReductaseEnzymeChemistryCofactorFlavin adenine dinucleotideTetrahydrobiopterinOxidative stressHemeDehydrogenaseSepiapterin reductaseBiopterinSepiapterinL-xylulose reductaseOne-electron reductionCytochrome b5 reductaseCytochrome b5DihydrobiopterinCytochromeSuperoxideMenadioneCarbonyl ReductaseReactive oxygen speciesAmino acidBiochemistryRedoxMetabolismDrug metabolism
5Publications
3H-index
41Citations
Publications 5
Newest
#1John T. Szilagyi (RU: Rutgers University)H-Index: 4
#2Karma C. Fussell (RU: Rutgers University)H-Index: 3
Last. Jeffrey D. Laskin (RU: Rutgers University)H-Index: 69
view all 11 authors...
Abstract NADH cytochrome b5 reductase mediates electron transfer from NADH to cytochrome b5 utilizing flavin adenine dinucleotide as a redox cofactor. Reduced cytochrome b5 is an important cofactor in many metabolic reactions including cytochrome P450-mediated xenobiotic metabolism, steroid biosynthesis and fatty acid metabolism, hemoglobin reduction, and methionine and plasmalogen synthesis. Using recombinant human enzyme, we discovered that cytochrome b5 reductase mediates redox cycling of a v...
3 CitationsSource
#1Shaojun Yang (RU: Rutgers University)H-Index: 3
#2Yi-Hua Jan (RU: Rutgers University)H-Index: 8
Last. Jeffrey D. Laskin (RU: Rutgers University)H-Index: 69
view all 6 authors...
Reactive carbonyls such as diacetyl (2,3-butanedione) and 2,3-pentanedione in tobacco and many food and consumer products are known to cause severe respiratory diseases. Many of these chemicals are detoxified by carbonyl reductases in the lung, in particular, dicarbonyl/l-xylulose reductase (DCXR), a multifunctional enzyme important in glucose metabolism. DCXR is a member of the short-chain dehydrogenase/reductase (SDR) superfamily. Using recombinant human enzyme, we discovered that DCXR mediate...
11 CitationsSource
#1Shaojun Yang (RU: Rutgers University)H-Index: 3
#2Yi-Hua Jan (RU: Rutgers University)H-Index: 8
Last. Jeffrey D. Laskin (RU: Rutgers University)H-Index: 69
view all 8 authors...
Sepiapterin reductase (SPR) catalyzes the reduction of sepiapterin to dihydrobiopterin (BH2), a precursor for tetrahydrobiopterin (BH4), a cofactor critical for nitric oxide biosynthesis and alkylglycerol and aromatic amino acid metabolism. SPR also mediates chemical redox cycling, catalyzing one electron reduction of redox active chemicals including quinones; rapid reaction of these radicals with molecular oxygen generates reactive oxygen species (ROS). Using recombinant human SPR, sulfonamide ...
Source
#1Shaojun Yang (RU: Rutgers University)H-Index: 3
#2Yi-Hua Jan (RU: Rutgers University)H-Index: 8
Last. Jeffrey D. Laskin (RU: Rutgers University)H-Index: 69
view all 9 authors...
Sepiapterin reductase (SPR) catalyzes the reduction of sepiapterin to dihydrobiopterin (BH2), the precursor for tetrahydrobiopterin (BH4), a cofactor critical for nitric oxide biosynthesis and alkylglycerol and aromatic amino acid metabolism. SPR also mediates chemical redox cycling, catalyzing one-electron reduction of redox-active chemicals, including quinones and bipyridinium herbicides (e.g., menadione, 9,10-phenanthrenequinone, and diquat); rapid reaction of the reduced radicals with molecu...
13 CitationsSource
#1Shaojun Yang (UMDNJ: University of Medicine and Dentistry of New Jersey)H-Index: 3
#2Yi Hua Jan (RU: Rutgers University)H-Index: 3
Last. Jeffrey D. Laskin (UMDNJ: University of Medicine and Dentistry of New Jersey)H-Index: 69
view all 7 authors...
Abstract In the lung, chemical redox cycling generates highly toxic reactive oxygen species that can cause alveolar inflammation and damage to the epithelium, as well as fibrosis. In this study, we identified a cytosolic NADPH-dependent redox cycling activity in mouse lung epithelial cells as sepiapterin reductase (SPR), an enzyme important for the biosynthesis of tetrahydrobiopterin. Human SPR was cloned and characterized. In addition to reducing sepiapterin, SPR mediated chemical redox cycling...
15 CitationsSource