Carina Bernardo
University of Aveiro
HRASMultiplex ligation-dependent probe amplificationCancerComparative genomic hybridizationOncologyEstrogen receptor alphaImmunologyGracilariaSchistosomiasisEstrogenSchistosoma haematobiumFluorescence in situ hybridizationLipidomePrimary tumorBladder cancerCancer researchCarcinogenesisMedicineEstrogen receptorBiology
Publications 10
#1Lúcio Lara SantosH-Index: 29
Last. José Manuel Correia da Costa (University of Porto)H-Index: 22
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Schistosomiasis is the most important helminthiasis worldwide in terms of morbidity and mortality. Most of the infections occurs in Africa, which about two thirds are caused by Schistosoma haematobium. The infection with S. haematobium is considered carcinogenic leading to squamous cell carcinoma (SCC) and urothelial carcinoma of the urinary bladder. Additionally, it is responsible for female genital schistosomiasis leading to infertility and higher risk of human immunodeficiency virus (HIV) tra...
1 CitationsSource
#1Carina Bernardo (University of Aveiro)H-Index: 5
#2Júlio Santos (University of Porto)H-Index: 5
Last. Lúcio Lara SantosH-Index: 29
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Abstract Estrogen-like metabolites have been identified in S. haematobium, the helminth parasite that causes urogenital schistosomiasis (UGS) and in patients´ blood and urine during UGS. Estrogen receptor (ER) activation is enriched in the luminal molecular subtype bladder cancer (BlaCa). To date, the significance of ER to these diseases remains elusive. We evaluated ERα and ERβ expression in UGS-related BlaCa (n = 27), UGS-related non-malignant lesions (n = 35), and noninfected BlaCa (n = 80). ...
3 CitationsSource
: Patient-derived xenograft (PDX ) tumors are models developed by direct transplant of human tumors into immune-compromised hosts such as nude mice. These models retain the histological and genetic characteristics of the primary tumor and are considered a valuable platform for translational cancer research. This chapter describes the methodology to establish and propagate bladder cancer PDX model.
1 CitationsSource
#1Carina Bernardo (University of Aveiro)H-Index: 5
#2Ana TavaresH-Index: 6
Last. Lúcio Lara SantosH-Index: 29
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e16013Background: Epidemiological and molecular findings suggest that estrogen may play a role in bladder carcinogenesis and in urogenital schistosomiasis (UGS). Methods: A comprehensive analysis o...
#1Elisabete da Costa (University of Aveiro)H-Index: 10
#2Tânia Melo (University of Aveiro)H-Index: 15
Last. Maria Rosário DominguesH-Index: 27
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The lipidome of the red seaweed Gracilaria sp., cultivated on land-based integrated multitrophic aquaculture (IMTA) system, was assessed for the first time using hydrophilic interaction liquid chromatography-mass spectrometry and tandem mass spectrometry (HILIC–MS and MS/MS). One hundred and forty-seven molecular species were identified in the lipidome of the Gracilaria genus and distributed between the glycolipids classes monogalactosyl diacylglyceride (MGDG), digalactosyl diacylglyceride (DGDG...
37 CitationsSource
#1Carina Bernardo (University of Aveiro)H-Index: 5
#2Maria Cláudia Cunha (University of Minho)H-Index: 1
Last. Lúcio Lara SantosH-Index: 29
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Infection due to Schistosoma haematobium is carcinogenic. However, the cellular and molecular mechanisms underlying urogenital schistosomiasis (UGS)-induced carcinogenesis have not been well defined. Conceptually, early molecular detection of this phenomenon, through non-invasive procedures, seems feasible and is desirable. Previous analysis of urine collected during UGS suggests that estrogen metabolites, including depurinating adducts, may be useful for this purpose. Here, a new direction was ...
13 CitationsSource
#1Carina Bernardo (University of Aveiro)H-Index: 5
#2Céu Costa (UFP: Fernando Pessoa University)H-Index: 8
Last. Lúcio Lara Santos (UFP: Fernando Pessoa University)H-Index: 29
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Patient-derived tumor xenografts (PDTXs) are said to accurately reflect the heterogeneity of human tumors. In the case of human bladder cancer, few studies are available featuring these models. The best methodology to develop and the real value of the model remain unclear. This systematic review aims to elucidate the best methodology to establish and use PDTXs to study the characteristics and behavior of human bladder tumors. The value and potential application of these models are also addressed...
20 CitationsSource
4 CitationsSource
#1Carina Bernardo (RMIT: RMIT University)H-Index: 5
#2Céu Costa (UFP: Fernando Pessoa University)H-Index: 8
Last. Lúcio Lara Santos (UFP: Fernando Pessoa University)H-Index: 29
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BACKGROUND: More than 70% of muscle invasive bladder cancers (MIBC) express the cell-surface antigen sialyl-Tn (sTn) that promotes motility and invasive potential of tumor cells. Effective drug testing models to optimize therapy against these tumors are warranted. MATERIALS AND METHODS: Fragments of sTn-positive MIBC were subcutaneously engrafted into nude mice and expanded until the third passage. Histology and immunoexpression of tumor markers (p53, p63, Ki-67, CK20, sTn) were studied in order...
21 Citations
#2Isabel M. Carreira (UC: University of Coimbra)H-Index: 25
Last. Lúcio Lara Santos (UFP: Fernando Pessoa University)H-Index: 29
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Several genomic regions are frequently altered and associated with the type, stage and progression of urinary bladder cancer (UBC). We present the characterization of 5637, T24 and HT1376 UBC cell lines by karyotyping, fluorescence in situ hybridization (FISH), array comparative genomic hybridization (aCGH) and multiplex ligation-dependent probe amplification (MLPA) analysis. Some cytogenetic anomalies present in UBC were found in the three cell lines, such as chromosome 20 aneuploidy and the lo...
27 CitationsSource
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