Yong-tie Li
Nanchang University
Induced pluripotent stem cellAmniotic stem cellsMoleH&E stainInternal medicineEndocrinologySurgeryEmbryoid bodyEmbryonic stem cellCellMolecular biologyAlkaline phosphataseAndrologyIn vitroImmunologySensory nerveSubstance PNerve fiberTrophoblastCollagenaseStreptozotocinWound healingType I collagenStem cellTransplantationDiabetes mellitusClone (cell biology)MedicineStainingBiologyEpidermis (botany)
Publications 4
#1Fei-bin Zhu (NCU: Nanchang University)H-Index: 1
#2Xiang-jing FangH-Index: 1
Last. De-ming Liu (NCU: Nanchang University)H-Index: 4
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Exogenous substance P accelerates wound healing in diabetes, but the mechanism remains poorly understood. Here, we established a rat model by intraperitoneally injecting streptozotocin. Four wounds (1.8 cm diameter) were drilled using a self-made punch onto the back, bilateral to the vertebral column, and then treated using amniotic membrane with epidermal stem cells and/or substance P around and in the middle of the wounds. With the combined treatment the wound-healing rate was 100% at 14 days....
5 CitationsSource
#1Yong-tie Li (NCU: Nanchang University)H-Index: 2
#2Dewu LiuH-Index: 3
Last. Qun-Hong YuH-Index: 1
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Abstract To investigate the feasibility of differentiation of human induced pluripotent stem cells (iPSCs) into epidermal-like stem cells. (1) Human strain of iPSCs were plated on-to trophoblast of inactivated Fb strain of mouse embryos and cultured in complete medium of embryonic stem cells, iPSCs were subcultured by collagenase IV digestion method. The morphology and growth of iPSCs were observed under inverted phase contrast microscope, and the cells were stained with alkaline phosphatase (AK...
3 Citations
#1Fei-bin Zhu (NCU: Nanchang University)H-Index: 1
#2Dewu LiuH-Index: 3
Last. Yong-tie LiH-Index: 2
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OBJECTIVE: To observe the effect of sensory neuropeptide substance P combined with epidermal stem cells (ESC) on wound healing and nerve regeneration in diabetic rats. METHODS: ESC that had been isolated from SD rats were identified and cultured in vitro, and they were inoculated onto nourishing layer of amniotic membrane to construct amniotic membrane-ESC. Four full-thickness skin wounds were produced on the back of each of 48 diabetic rats. The resulted 192 wounds were randomly divided into ES...
1 Citations
#1Fei-bin Zhu (NCU: Nanchang University)H-Index: 1
#2Dewu Liu (NCU: Nanchang University)H-Index: 6
Last. Pu Ning (NCU: Nanchang University)H-Index: 3
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Epidermal stem cells (ESCs) play a critical roles in wound healing. But the biological characteristics of ESCs derived from diabetes mellitus are not fully understood. In this chapter, we compared the biological characteristics between diabetes and normal skin derived ESCs in vitro. The diabetes mellitus (DM) rat models were made by intraperitoneal injected streptozotocin. ESCs were extracted from the dorsal skin samples of rats by trypsin digesting method and further purified by collagen adheri...