Halina Abramczyk
Lodz University of Technology
Analytical chemistryAbsorption spectroscopyCancerPhysical chemistryInfrared spectroscopyFemtosecondChemistryMaterials scienceMethylcyclohexaneRaman imagingSpectroscopyMoleculeRaman spectroscopyMolecular physicsSolvated electronNuclear magnetic resonanceDephasingCancer researchBreast cancerPhotochemistryVibrational energy relaxation
124Publications
26H-index
2,007Citations
Publications 121
Newest
#1Monika Kopec (TUL: Lodz University of Technology)H-Index: 11
#2Maciej Błaszczyk (Medical University of Łódź)H-Index: 3
Last. Halina Abramczyk (TUL: Lodz University of Technology)H-Index: 26
view all 4 authors...
Abstract null null Spectroscopic methods provide information on the spatial localization of biochemical components based on the analysis of vibrational spectra. Raman spectroscopy and Raman imaging can be used to analyze various types of human brain tumors and breast cancers. The objective of this study is to evaluate the Raman biomarkers to distinguish tumor types by Raman spectroscopy and Raman imaging. We have demonstrated that bands characteristic for carotenoids (1156 cm−1, 1520 cm−1), prot...
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#1Halina Abramczyk (TUL: Lodz University of Technology)H-Index: 26
#2Beata Brozek-Pluska (TUL: Lodz University of Technology)H-Index: 18
Last. Monika Kopec (TUL: Lodz University of Technology)H-Index: 11
view all 3 authors...
Cytochrome c (Cyt c) is a key protein that is needed to maintain life (respiration) and cell death (apoptosis). The dual-function of Cyt c comes from its capability to act as mitochondrial redox carrier that transfers electrons between the membrane-embedded complexes III and IV and to serve as a cytoplasmic apoptosis-triggering agent, activating the caspase cascade.1-6 However, the precise roles of Cyt c in mitochondria, cytoplasm and extracellular matrix under normal and pathological conditions...
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#1Halina Abramczyk (TUL: Lodz University of Technology)H-Index: 26
#2Jakub Surmacki (TUL: Lodz University of Technology)H-Index: 14
Last. Monika Kopec (TUL: Lodz University of Technology)H-Index: 11
view all 4 authors...
We used Raman imaging to monitor changes in the redox state of the mitochondrial cytochromes in ex vivo human brain and breast tissues, surgically resected specimens of human tissues and in vitro human brain cells of normal astrocytes (NHA), astrocytoma (CRL-1718), glioblastoma (U87-MG) and medulloblastoma (Daoy), and human breast cells of normal cells (MCF 10A), slightly malignant cells (MCF7) and highly aggressive cells (MDA-MB-231) by means of Raman microspectroscopy at 532 nm. We visualized ...
1 CitationsSource
#1Monika KopecH-Index: 11
#2Maciej BłaszczykH-Index: 3
Last. Halina AbramczykH-Index: 26
view all 4 authors...
Spectroscopic methods provide information on the spatial localization of biochemical components based on the analysis of vibrational spectra. Raman spectroscopy and Raman imaging can be used to analyze various types of human brain tissue. The objective of this study is employment of Raman spectroscopy and Raman imaging to evaluate the Raman biomarkers to distinguish tumor types. We have demonstrated that bands characteristic for carotenoids (1156 cm-1, 1520 cm-1), proteins (1004 cm-1), fatty aci...
Source
#1Halina AbramczykH-Index: 26
#2Beata Brozek-PluskaH-Index: 18
Last. Maciej RadekH-Index: 9
view all 6 authors...
To monitor redox state changes and biological mechanisms occurring in mitochondrial cytochromes in cancers improving methods are required. We used Raman spectroscopy and Raman imaging to monitor changes in the redox state of the mitochondrial cytochromes in ex vivo human brain and breast tissues at 532 nm, 633 nm, 785 nm. We identified the oncogenic processes that characterize human infiltrating ductal carcinoma (IDC) and human brain tumors: gliomas; astrocytoma and medulloblastoma based on the ...
4 CitationsSource
#1Halina Abramczyk (TUL: Lodz University of Technology)H-Index: 26
#2Beata Brozek-Pluska (TUL: Lodz University of Technology)H-Index: 18
Last. Maciej Radek (Medical University of Łódź)H-Index: 9
view all 5 authors...
BackgroundTo monitoring redox state changes and biological mechanisms occurring in mitochondrial cytochromes in cancers improving novel methods are required. MethodsWe used Raman spectroscopy and Raman imaging to monitor changes in the redox state of the mitochondrial cytochromes in ex vivo human brain and breast tissues at 532 nm, 633 nm, 785 nm. ResultsWe identified the oncogenic processes that characterize human infiltrating ductal carcinoma (IDC) and human brain tumors: gliomas; astrocytoma ...
2 CitationsSource
#1Anna Imiela (TUL: Lodz University of Technology)H-Index: 7
#2Jakub Surmacki (TUL: Lodz University of Technology)H-Index: 14
Last. Halina Abramczyk (TUL: Lodz University of Technology)H-Index: 26
view all 3 authors...
Abstract We developed a label-free Raman method for monitoring the therapeutic benefit of temozolomide in glioblastoma by whole-cell biochemical hyperspectral imaging for detecting molecular processes occurring in normal and tumorous brain cells at the level of isolated organelles. Our approach allows biochemical compositional mapping of lipid droplets, mitochondria, and nucleus in single cells, monitoring intracellular alterations upon using different doses of temozolomide, a drug normally used...
1 CitationsSource
#1Beata Brozek-Pluska (TUL: Lodz University of Technology)H-Index: 18
#2Arkadiusz Jarota (TUL: Lodz University of Technology)H-Index: 5
Last. Halina Abramczyk (TUL: Lodz University of Technology)H-Index: 26
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Photodynamic therapy is a clinically approved alternative method for cancer treatment in which a combination of nontoxic drugs known as photosensitizers and oxygen is used. Despite intensive investigations and encouraging results, zinc phthalocyanines (ZnPcs) have not yet been approved as photosensitizers for clinical use. Label-free Raman imaging of nonfixed and unstained normal and cancerous colon human tissues and normal human CCD18-Co and cancerous CaCo-2 cell lines, without and after adding...
3 CitationsSource
#1Halina Abramczyk (TUL: Lodz University of Technology)H-Index: 26
#2Jakub Surmacki (TUL: Lodz University of Technology)H-Index: 14
Last. Beata Brozek-Pluska (TUL: Lodz University of Technology)H-Index: 18
view all 3 authors...
This paper presents a non-invasive approach to study redox status of cytochromes in vitro human brain cells of normal astrocytes (NHA), astrocytoma (CRL-1718), glioblastoma (U87-MG) and medulloblastoma (Daoy), and human breast cells of normal cells (MCF10A), slightly malignant cells (MCF7) and highly aggressive cells (MDA-MB-231), in vivo animal models, and ex vivo brain and breast tissues surgically resected human specimens by means of Raman microspectroscopy at 355 nm, 532 nm, 785 nm and endos...
5 CitationsSource
#1Halina Abramczyk (TUL: Lodz University of Technology)H-Index: 26
#2Anna Imiela (TUL: Lodz University of Technology)H-Index: 7
Last. Jakub Surmacki (TUL: Lodz University of Technology)H-Index: 14
view all 3 authors...
We developed a label-free Raman method for whole-cell biochemical imaging to detect molecular processes that occur in normal and cancer brain cells due to retinol transport in human cancers at the level of isolated organelles. Our approach allows the creation of biochemical maps of lipid droplets, mitochondria and nuclei in single cells. The maps were capable of discriminating triglycerides (TAG) from retinyl ester (RE) in lipid droplets (LD), providing an excellent tool to monitor intracellular...
2 CitationsSource