Alison Obergrussberger
Analytical chemistryBiophysicsBiomedical engineeringPatch clampEmbedded systemTransient receptor potential channelAutomated patch clampReceptorElectrophysiologyChemistryMaterials scienceClampSafety pharmacologyCurrent clamphERGComputer scienceIon channelDrug discoveryLigand-gated ion channelCell biologyPharmacology
33Publications
9H-index
217Citations
Publications 34
Newest
Abstract null null Introduction null For reliable identification of cardiac safety risk, compounds should be screened for activity on cardiac ion channels in addition to hERG, including NaV1.5 and CaV1.2. We identified different parameters that might affect IC50s of compounds on NaV1.5 peak and late currents recorded using automated patch clamp (APC) and suggest outlines for best practices. null null null Methods null APC instruments SyncroPatch 384 and Patchliner were used to record NaV1.5 peak...
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Although automated patch clamp (APC) devices have been around for many years and have become an integral part of many aspects of drug discovery, high throughput instruments with gigaohm seal data quality are relatively new. Experiments where a large number of compounds are screened against ion channels are ideally suited to high throughput APC, particularly when the amount of compound available is low. Here we evaluate different APC approaches using a variety of ion channels and screening settin...
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#1Andrea BrüggemannH-Index: 29
#2Tom A. GoetzeH-Index: 13
Last. Niels FertigH-Index: 28
view all 10 authors...
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Patch-clamp electrophysiology remains an important technique in studying ion channels; indeed, it is still considered the gold standard since it was first described by Neher and Sakmann in the 1970...
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Artificial lipid bilayers have been used for several decades to study channel-forming pores and ion channels in membranes. Until recently, the classical two-chamber setups have been primarily used for studying the biophysical properties of pore forming proteins. Within the last 10 years, instruments for automated lipid bilayer measurements have been developed and are now commercially available. This chapter focuses on protein purification and reconstitution of channel-forming proteins into lipid...
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