Robbie Yat-Kan Chan
Hong Kong Baptist University
MCF-7Biological activityEndocrinologyChemistryEstrogenCurcumaHeLaCholesterolFatty liverLipoproteinChlorogenic acidAllicinNormal dietCancer researchMedicineEstrogen receptorBiologyEstrogen receptor betaProtein kinase APharmacology
18Publications
11H-index
700Citations
Publications 18
Newest
#1Robbie Yat-Kan Chan (Hong Kong Baptist University)H-Index: 11
#2Kenneth Ka Ho Lee (CUHK: The Chinese University of Hong Kong)H-Index: 24
Last. Chung-Hin Chui (PolyU: Hong Kong Polytechnic University)H-Index: 10
view all 10 authors...
Ethanol has been commonly used as a vehicle for drug discovery purpose in vitro. The human breast cancer MCF-7 estrogen dependent cell line is a common in vitro model used for hormonal therapy study. However, special precaution is suggested when ethanol is used in pharmacological tests as solvent in order to evaluate the biological activity of potential drugs especially concerning about the MCF-7. Ethanol was shown to stimulate the proliferation of this estrogen receptor positive cell line. Here...
Source
#1Jackie Yan-Yan Chan (PolyU: Hong Kong Polytechnic University)H-Index: 2
#2Hei-Tung Tsui (PolyU: Hong Kong Polytechnic University)H-Index: 1
Last. Shun Wan Chan (PolyU: Hong Kong Polytechnic University)H-Index: 33
view all 6 authors...
AbstractOxidative stress is considered an important factor that promotes cell death in response to a variety of pathophysiological conditions. This study investigated the antioxidant properties of allicin, the principle ingredient of garlic, on preventing oxidative stress-induced injury. The antioxidant capacities of allicin were measured by using 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay and hydrogen peroxide (H2O2)-induced cell damage on H9c2 cardiomyoblasts. Allicin (0....
13 CitationsSource
#1Wing-Yan Li (PolyU: Hong Kong Polytechnic University)H-Index: 4
#2Robbie Yat-Kan Chan (Hong Kong Baptist University)H-Index: 11
Last. Shun Wan Chan (PolyU: Hong Kong Polytechnic University)H-Index: 33
view all 4 authors...
AbstractContext: The poor prognostic outcome of breast cancer is largely due to its resistance to cancer therapies. Development of therapeutic agents that can inhibit growth and induce apoptosis in breast cancer cells can help solve the problem. Emodin is an active anthraquinone that has been reported to have diverse biological effects.Objective: In this study, the anticancer effects of emodin on growth inhibition, apoptosis induction and the expression of apoptosis-related genes in MCF-7 cells ...
17 CitationsSource
#1Jackie Yan Yan Chan (PolyU: Hong Kong Polytechnic University)H-Index: 1
Last. Shun Wan Chan (PolyU: Hong Kong Polytechnic University)H-Index: 33
view all 4 authors...
Abstract Cardiovascular disease (CVD) is a category of chronic noncommunicable diseases causing high global mortality and has been a heavy social burden in many countries. In the search of chemicals that arise from natural food source, allicin is one such ingredient from garlic that was discovered with the potential to provide beneficial effects to the cardiovascular system. From the pharmacokinetic studies, allicin is known to be hydrophobic and can be readily absorbed through the cell membrane...
109 CitationsSource
#1Chun-Wai Wan (PolyU: Hong Kong Polytechnic University)H-Index: 3
#2Candy Ngai-Yan Wong (PolyU: Hong Kong Polytechnic University)H-Index: 2
Last. Shun Wan Chan (PolyU: Hong Kong Polytechnic University)H-Index: 33
view all 8 authors...
Hypercholesterolemia is a major risk factor for the development of cardiovascular disease and nonalcoholic fatty liver disease. Natural compounds have been proved to be useful in lowering serum cholesterol to slow down the progression of cardiovascular disease and nonalcoholic fatty liver disease. In the present study, the hypocholesterolemic and hepatoprotective effects of the dietary consumption of chlorogenic acid were investigated by monitoring plasma lipid profile (total cholesterol, trigly...
119 CitationsSource
#1Wai-Man Tang (PolyU: Hong Kong Polytechnic University)H-Index: 1
#2Enoch Chan (PolyU: Hong Kong Polytechnic University)H-Index: 6
Last. Shun Wan Chan (PolyU: Hong Kong Polytechnic University)H-Index: 33
view all 9 authors...
The anticancer effects of traditional Chinese medicine (TCM) have attracted the attention of the public vis-a-vis existing cancer therapies with various side effects. Lycium barbarum fruit, commonly known as Gou Qi Zi in China, is a potential anticancer agent/adjuvant. Its major active ingredients, L. barbarum polysaccharides (LBP), scopoletin and 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), are found to have apoptotic and antiproliferative effects on cancer cell lines. Moreover, LBP also co...
79 CitationsSource
#1Tsz-Shan Kam (PolyU: Hong Kong Polytechnic University)H-Index: 2
#2Cho-Yee WongH-Index: 2
Last. Robbie Yat-Kan ChanH-Index: 11
view all 8 authors...
Abstract An extract of Curcuma longa was tested in hypercholesterolemic rats to investigate its potential therapeutic effect on vascular conditions. Four experimental groups were used: normal diet (ND) control group, high cholesterol diet (HCD) group, and HCD subgroups supplemented with turmeric extract at 100 or 300 mg/kg of body weight (HCD100Tur and HCD300Tur groups, respectively). Turmeric extract was fed orally to animals, and dietary treatments lasted for 28 days. Hypercholesterolemia deve...
8 CitationsSource
#1Janet Yuen Yan Chan (PolyU: Hong Kong Polytechnic University)H-Index: 2
#2Enoch Chan (PolyU: Hong Kong Polytechnic University)H-Index: 6
Last. Ivan Ying Ming ChungH-Index: 2
view all 9 authors...
Context: Grifola frondosa (Polyporaceae), maitake, is a widely consumed edible mushroom in some Asian countries. The fruit bodies and mycelia of maitake have shown different bioactive compounds with anticancer and other therapeutic properties.Objective: This study evaluated three chemically modified maitake polysaccharide-peptides’ (MPSP) adjuvant effect (in vivo) and anticancer activity (in vitro growth inhibitory effect) compared with crude MPSP from G. frondosa.Materials and methods: We inves...
12 CitationsSource
#1Wing-Fat Yiu (PolyU: Hong Kong Polytechnic University)H-Index: 1
#2Pui-Long Kwan (PolyU: Hong Kong Polytechnic University)H-Index: 2
Last. Robbie Yat-Kan Chan (PolyU: Hong Kong Polytechnic University)H-Index: 11
view all 7 authors...
: The extract of Curcuma longa, better known as turmeric, was orally administered to experimental rats that were fed a high-cholesterol diet to investigate whether it could regulate plasma lipids and cholesterol levels and possibly improve hepatic conditions. With turmeric supplements, rats showed a significant decrease in total plasma cholesterol and low-density lipoprotein cholesterol but an increase in high-density lipoprotein cholesterol when compared with rats that were fed a high-cholester...
43 CitationsSource
#1Chun-Wai Wan (PolyU: Hong Kong Polytechnic University)H-Index: 3
#2Yee-Ki Lee (PolyU: Hong Kong Polytechnic University)H-Index: 2
Last. Shun Wan Chan (PolyU: Hong Kong Polytechnic University)H-Index: 33
view all 6 authors...
A validated high performance liquid chromatographic (HPLC) method with dual wavelength detection was developed and applied to the determination of chlorogenic acid in rat plasma. Elution was performed on C18 column at 27°C with acetronitrile–water (0.2% phosphoric acid, pH = 2.0) at different proportions according to a time scheduled programme and pumped at a flow rate of 1.0 mL min−1. The eluent was detected at 325 nm for chlorogenic acid and 370 nm for the internal standard, kaempferol. The in...
2 CitationsSource
12
Close Researchers