Erica L. Fletcher
University of Melbourne
OphthalmologyInternal medicineEndocrinologyPathologyNeuroscienceReceptorChemistryMacular degenerationRetinaRetinal pigment epitheliumAmacrine cellDrusenElectroretinographyDiabetic retinopathyRetinalRetinal degenerationInner plexiform layerRetinitis pigmentosaAge relatedDiabetes mellitusMedicineBiologyCell biology
Publications 223
#1Michael A. Dixon (University of Melbourne)H-Index: 3
#2Ursula Greferath (University of Melbourne)H-Index: 26
Last. Andrew I. Jobling (University of Melbourne)H-Index: 21
view all 4 authors...
Microglia, the resident immune cells of the central nervous system (CNS), were once considered quiescent cells that sat in readiness for reacting to disease and injury. Over the last decade, however, it has become clear that microglia play essential roles in maintaining the normal nervous system. The retina is an easily accessible part of the central nervous system and therefore much has been learned about the function of microglia from studies in the retina and visual system. Anatomically, micr...
#1Ben J. Gu (Florey Institute of Neuroscience and Mental Health)H-Index: 28
#1Ben J. Gu (Fudan University)
Last. Erica L. Fletcher (University of Melbourne)H-Index: 51
view all 15 authors...
Age-related macular degeneration (AMD) is characterized by the accumulation of debris in the posterior eye. In this study we evaluated peripheral blood monocyte phagocytic function at various stages of AMD and in aged matched control participants. Real-time tri-colour flow cytometry was used to quantify phagocytic function of peripheral blood monocyte subsets (non-classic, intermediate and classic) isolated from subjects with intermediate or late AMD and compared with age matched healthy control...
#1Anna Y. M. Wang (University of Melbourne)
#2Vickie H. Y. Wong (University of Melbourne)H-Index: 11
Last. Erica L. Fletcher (University of Melbourne)H-Index: 51
view all 7 authors...
There is increasing evidence for the vulnerability of specific retinal ganglion cell (RGC) types in those with glaucoma and in animal models. In addition, the P2X7-receptor (P2X7-R) has been suggested to contribute to RGC death following stimulation and elevated IOP, though its role in RGC dysfunction prior to death has not been examined. Therefore, we examined the effect of an acute, non-ischemic intraocular pressure (IOP) insult (50 mmHg for 30 min) on RGC function in wildtype mice and P2X7-R ...
#1Amy C CohnH-Index: 10
#2Zhichao WuH-Index: 21
Last. Robyn H. GuymerH-Index: 71
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The presence of drusen is an important hallmark of age-related macular degeneration (AMD). Laser-induced regression of drusen, first observed over four decades ago, has led to much interest in the potential role of lasers in slowing the progression of the disease. In this article, we summarise the key insights from pre-clinical studies into the possible mechanisms of action of various laser interventions that result in beneficial changes in the retinal pigment epithelium/Bruch’s membrane/chorioc...
#1Una Greferath (University of Melbourne)H-Index: 2
#2Mario Huynh (University of Melbourne)H-Index: 1
Last. Erica L. Fletcher (University of Melbourne)H-Index: 51
view all 9 authors...
Retinitis pigmentosa is a family of inherited retinal degenerations associated with gradual loss of photoreceptors, that ultimately leads to irreversible vision loss. The Royal College of Surgeon’s (RCS) rat carries a recessive mutation affecting mer proto-oncogene tyrosine kinase (merTK), that models autosomal recessive disease. The aim of this study was to understand the glial, microglial and photoreceptor changes that occur in different retinal locations with advancing disease. Pigmented RCS ...
#1Grace E. Lidgerwood (University of Melbourne)H-Index: 12
#2Anne Senabouth (Garvan Institute of Medical Research)H-Index: 10
Last. Alice Pébay (University of Melbourne)H-Index: 31
view all 11 authors...
Human pluripotent stem cell (hPSC)-derived progenies are immature versions of cells, presenting a potential limitation to the accurate modelling of diseases associated with maturity or age. Hence, it is important to characterise how closely cells used in culture resemble their native counterparts. In order to select appropriate time points of retinal pigment epithelium (RPE) cultures that reflect native counterparts, we characterised the transcriptomic profiles of the hPSC-derived RPE cells from...
#1Kiana Kakavand (University of Melbourne)H-Index: 1
#2Andrew I. Jobling (University of Melbourne)H-Index: 21
Last. Erica L. Fletcher (University of Melbourne)H-Index: 51
view all 6 authors...
Photoreceptor death contributes to 50% of irreversible vision loss in the western world. Pro23His (P23H) transgenic albino rat strains are widely used models for the most common rhodopsin gene mutation associated with the autosomal dominant form of retinitis pigmentosa. However, the mechanism(s) by which photoreceptor death occurs are not well understood and were the principal aim of this study. We first used electroretinogram recording and optical coherence tomography to confirm the time course...
1 CitationsSource
Photoreceptor death is an important contributor to irreversible vision loss worldwide. In this review, I outline our work examining the role that purines, such as adenosine triphosphate (ATP), have in normal retinal function and in retinal disease. Our work shows that the actions of ATP, mediated by P2X receptors, are expressed in various retinal layers including photoreceptor terminals, and when stimulated by excessive levels of ATP is associated with rapid death of neurons. Treatment with a co...
1 CitationsSource
#1Anna Ym Wang (University of Melbourne)H-Index: 1
#2Pei Ying Lee (University of Melbourne)H-Index: 7
Last. Kirstan A. Vessey (University of Melbourne)H-Index: 26
view all 10 authors...
Glaucoma is a neurodegenerative disease characterised by progressive damage to the retinal ganglion cells (RGCs), the output neurons of the retina. RGCs are a heterogenous class of retinal neurons which can be classified into multiple types based on morphological, functional and genetic characteristics. This review examines the body of evidence supporting type-specific vulnerability of RGCs in glaucoma and explores potential mechanisms by which this might come about. Studies of donor tissue from...
5 CitationsSource
#1Samuel A. Mills (University of Melbourne)H-Index: 6
#2Andrew I. Jobling (University of Melbourne)H-Index: 21
Last. Erica L. Fletcher (University of Melbourne)H-Index: 51
view all 17 authors...
Local blood flow control within the CNS is critical to proper function and is dependent on coordination between neurons, glia and blood vessels. Macroglia such as astrocytes and Muller cells, contribute to this neurovascular unit within the brain and retina, respectively. This study explored the role of microglia, the innate immune cell of the CNS, in retinal vasoregulation and highlights changes during early diabetes. Structurally, microglia were found to contact retinal capillaries and neurona...