Alessandra Bossi
University of Verona
Surface plasmon resonanceAnalytical chemistryNanoparticleBiosensorMolecularly imprinted polymerNanotechnologyChemistryMaterials scienceHepcidinPeptideCapillary electrophoresisImmobilized enzymeMolecular imprintingElectrophoresisMembraneChemical engineeringChromatographyPolymerizationIsoelectric focusingPolymerBiochemistryIsoelectric point
103Publications
28H-index
3,002Citations
Publications 104
Newest
#1Alessandra Bossi (University of Verona)H-Index: 28
Last. Devid Maniglio (University of Trento)H-Index: 24
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Nanosized biomimetics prepared by the strategy of molecular imprinting, that is, the stamping of recognition sites by means of a template-assisted synthesis, are demonstrating potential as plastic antibodies in medicine, proving effective for cell imaging and targeted therapies. Most molecularly imprinted nanoparticles (MIP-NPs) are currently made of soft matter, such as polyacrylamide and derivatives. Yet, MIP-NPs biocompatibility is crucial for their effective translation into clinical uses. H...
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The molecular imprinting of proteins is the process of forming biomimetics with entailed protein-recognition by means of a template-assisted synthesis. Protein-imprinted polymers (pMIPs) have been successfully employed in separations, assays, sensors, and imaging. From a technical point of view, imprinting a protein is both costly, for protein expression and purification, and challenging, for the preservation of the protein's structural properties. In fact, the imprinting process needs to guaran...
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#1Laura PasquardiniH-Index: 15
#2Nunzio CennamoH-Index: 18
Last. Alessandra BossiH-Index: 28
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Postoperative pancreatic fistula (POPF), the major driver of morbidity and mortality following pancreatectomy, is caused by an abnormal communication between the pancreatic ductal epithelium and another epithelial surface containing pancreas-derived, enzyme-rich fluid. There is a strong correlation between the amylase content in surgically-placed drains early in the postoperative course and the development of POPF. A simple and cheap method to determine the amylase content from the drain effluen...
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Epitope imprinting is an effective strategy to prepare molecularly imprinted polymers (MIPs) for protein recognition. Indeed, the idea to use as a template just a fragment of the protein of interest, called the epitope, instead of the whole protein, presents some key advantages for the imprinting process, in particular: cutting the costs for MIP production and avoiding protein unfolding during the imprinting process, so to ultimately improve the quality of the stamped binding sites. How to selec...
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Molecularly imprinted nanoparticles (MIP NPs) are antibody-like recognition materials prepared by a template assisted synthesis. MIP NPs able to target biomolecules, like proteins, are under the spotlight for their great potential in medicine, but using the molecular imprinting technique with biological macromolecular templates efficiently is still very challenging. Here we propose generating a molecular imprint in single NPs, by photochemically initiating the polymerization from individual prot...
2 CitationsSource
#1A. Chiappini (fondazione bruno kessler)H-Index: 12
#1Andrea Chiappini (fondazione bruno kessler)H-Index: 22
Last. Alessandra BossiH-Index: 28
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Optical sensing, taking advantage of the variety of available optical structures, is a rapidly expanding area. Over recent years, whispering gallery mode resonators, photonic crystals, optical waveguides, optical fibers and surface plasmon resonance have been exploited to devise different optical sensing configurations. In the present review, we report on the state of the art of optical sensing devices based on the aforementioned optical structures and on synthetic receptors prepared by means of...
4 CitationsSource
#1Anna Laura Capriotti (Sapienza University of Rome)H-Index: 38
#2Susy Piovesana (Sapienza University of Rome)H-Index: 27
Last. Aldo Laganà (Sapienza University of Rome)H-Index: 61
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Abstract “Plastic antibodies” are nano-sized biomimetics prepared by the molecular imprinting technology, which have the robustness of polymers, but specificity and selectivity alike natural receptors making them ideal for analytical uses. The current challenge is to translate plastic antibodies to in vivo applications for diagnosis, drug delivery, theranostic, therefore it is crucial to evaluate the effect of the biological sample complexity on the selectivity and the formation of protein coron...
4 CitationsSource
#1Nunzio CennamoH-Index: 18
#2Devid Maniglio (University of Trento)H-Index: 24
Last. Alessandra Bossi (University of Verona)H-Index: 28
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Abstract Soft molecularly imprinted nanogels (nanoMIPs), selective for human transferrin (HTR), were prepared via a template assisted synthesis. Owing to their soft matter, the nanoMIPs were observed to deform at binding to HTR: while no relevant changes were observed in the hydrodynamic sizes of HTR-free compared to HTR-loaded nanoMIPs, the HTR binding resulted in a significant increment of the nanoMIP stiffness, with the mean Young's modulus measured by AFM passing from 17 ± 6 kPa to 56 ± 18 k...
7 CitationsSource
#1Stanislav S. Piletsky (Imperial College London)H-Index: 5
Last. Sergey A. Piletsky (University of Leicester)H-Index: 77
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Since their conception 50 years ago, molecularly imprinted polymers (MIPs) have seen extensive development both in terms of synthetic routes and applications. Cells are perhaps the most challenging target for molecular imprinting. Although early work was based almost entirely around microprinting methods, recent developments have shifted towards epitope imprinting to generate MIP nanoparticles (NPs). Simultaneously, the development of techniques such as solid phase MIP synthesis has solved many ...
46 CitationsSource
#1Alessandra Bossi (University of Verona)H-Index: 28
Monoclonal antibodies have shown tremendous success in cancer treatment; however, humanization for clinical applications is expensive and not straightforward. Now, molecularly imprinted polymer nanogels have been developed that can block cell-surface proteins and disrupt tumour spheroids.
7 CitationsSource