R. John Collier
Harvard University
EndosomeBiophysicsChromosomal translocationMutantMolecular biologyReceptorChemistryTransmembrane proteinToxinBacillus anthracisDiphtheria toxinLipid bilayerMembraneAnthrax toxinBiochemistryTransport proteinStereochemistryBiologyMicrobiologyCell biologyCytosol
148Publications
63H-index
8,919Citations
Publications 146
Newest
#1Alexander R. Loftis (MIT: Massachusetts Institute of Technology)H-Index: 6
#2Genwei Zhang (MIT: Massachusetts Institute of Technology)H-Index: 4
Last. Bradley L. Pentelute (MIT: Massachusetts Institute of Technology)H-Index: 30
view all 12 authors...
Targeting of antigens to erythrocytes can be used to selectively mitigate their immunogenicity, but the methods to equip a variety of cargoes with erythrocyte-targeting properties are limited. Here we identified a D-peptide that targets murine erythrocytes and decreases anti-drug antibody responses when conjugated to the protective antigen from Bacillus anthracis, a protein of therapeutic interest. The D-peptide likewise decreases inflammatory anti-ovalbumin (OVA) CD8+ T cell responses when atta...
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#1Nicole J. Yang (Harvard University)H-Index: 3
#2Jörg Isensee (University of Cologne)H-Index: 15
Last. Isaac M. Chiu (Harvard University)H-Index: 33
view all 21 authors...
Bacterial toxins are able to act on neurons to modulate signaling and function. Here, we find that nociceptive sensory neurons that mediate pain are enriched in the receptor for anthrax toxins, ANTXR2. Anthrax Edema Toxin (ET) induced cAMP and PKA signaling in Nav1.8+ nociceptive neurons and modulated pain in vivo. Peripherally administered ET mediated mechanical allodynia in naive mice and during B. anthracis infection. Intrathecally administered ET produced analgesic effects, potently blocking...
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#1Nicole J. YangH-Index: 3
#2Sai Man LiuH-Index: 9
Last. Isaac M. ChiuH-Index: 33
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1 CitationsSource
#1R. John Collier (Harvard University)H-Index: 63
#2Arturo Casadevall (Johns Hopkins University)H-Index: 137
Over the past 2 years, we have made a concerted effort to expand the number and scope of minireviews published in mBio in order to help readers keep track of developments across the breadth of microbiology. In our conversations exploring potential subjects for …
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#1Jiansen Jiang (UCLA: University of California, Los Angeles)H-Index: 20
#2Bradley L. Pentelute (MIT: Massachusetts Institute of Technology)H-Index: 30
Last. Z. Hong Zhou (UCLA: University of California, Los Angeles)H-Index: 63
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Anthrax toxin, the major virulence factor of Bacillus anthracis causing high mortality in human and animals, comprises protective antigen (PA), lethal factor (LF) and edema factor (EF). PA plays a central role in the toxicity by translocating the enzymes LF and EF into the cytosol, which makes it not only a vaccine component and therapeutic target for anthrax infections but also an excellent model system for understanding the mechanism of protein translocation. PA first forms soluble oligomeric ...
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#1Stephen LoryH-Index: 84
#2R. John CollierH-Index: 63
We have used flow dialysis to demonstrate binding of ATP and related compounds to diphtheria toxin. The results define a new site on the toxin molecule (the P site), which has distinctly different properties from the NAD+-binding site of the fragment A moiety. The relative affinities of various compounds for the P site are similar to their capacities to inhibit toxin attachment to cell surfaces and its action on cells. This suggests that the P site may correspond to the binding site for cell sur...
#1Robert J. Collier (Harvard University)H-Index: 96
#1R. John Collier (Harvard University)H-Index: 63
In the summer of 2014, the community of scientists studying bacterial protein toxins lost one of its pioneers and most prolific members, Sjur Olsnes. Sjur was a valued friend and colleague to those of us who knew and worked with him, and his passing was a deep loss. Sjur was born in Bergen, Norway, in 1939 and grew up on a farm nearby. He studied medicine in Bonn and after a short period at the University of Bergen moved to Oslo, where he did doctoral research in the Institute for Cancer Researc...
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#1Jiansen Jiang (UCLA: University of California, Los Angeles)H-Index: 20
#2Bradley L. Pentelute (MIT: Massachusetts Institute of Technology)H-Index: 30
Last. Z. Hong Zhou (UCLA: University of California, Los Angeles)H-Index: 63
view all 4 authors...
Cryo-electron microscopy determination of anthrax toxin protective antigen pore structure at a resolution of 2.9 A, revealing the catalytic Φ-clamp and the membrane-spanning translocation channel.
164 CitationsSource
#1Narahari Akkaladevi (KU: University of Kansas)H-Index: 7
#2Srayanta Mukherjee (KU: University of Kansas)H-Index: 9
Last. Mark T. Fisher (KU: University of Kansas)H-Index: 19
view all 9 authors...
Bacterial toxin or viral entry into the cell often requires cell surface binding and endocytosis. The endosomal acidification induces a limited unfolding/refolding and membrane insertion reaction of the soluble toxins or viral proteins into their translocation competent or membrane inserted states. At the molecular level, the specific orientation and immobilization of the pre-transitioned toxin on the cell surface is often an important prerequisite prior to cell entry. We propose that structures...
10 CitationsSource
#2Srayanta MukherjeeH-Index: 9
Last. Bradley L. PenteluteH-Index: 30
view all 9 authors...