Anne Moscona
Columbia University
Lipid bilayer fusionGlycoproteinHeptad repeatFusion proteinNeuraminidaseVirologyReceptorChemistryImmunologyIn vivoVirusHemagglutinin-neuraminidaseMeasles virusHemagglutinin (influenza)ParamyxoviridaeHN ProteinHuman Parainfluenza VirusBiochemistryViral entryMedicineBiologyCell biologyViral envelope
Publications 123
#1Debora Stelitano (Columbia University)H-Index: 3
#2Stuart P. Weisberg (Columbia University)H-Index: 17
Last. Michele Cennamo (University of Naples Federico II)H-Index: 4
view all 18 authors...
The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is ongoing and has shown the community that flexible methods for rapidly identifying and screening candidate antivirals are needed. Assessing virus-neutralizing activity of human serum to monitor population immunity and response to infection and vaccination is key to pandemic control. We developed a virus neutralization platform strategy that relies only on bioinformatic and genetic information of t...
#1Francesca T. Bovier (Columbia University)H-Index: 6
#2Ksenia Rybkina (Columbia University)H-Index: 4
Last. Matteo Porotto (Columbia University)H-Index: 29
view all 9 authors...
Measles virus (MeV) infection remains a significant public health threat despite ongoing global efforts to increase vaccine coverage. As eradication of MeV stalls, and vulnerable populations expand, effective antivirals against MeV are in high demand. Here, we describe the development of an antiviral peptide that targets the MeV fusion (F) protein. This antiviral peptide construct is composed of a carbobenzoxy-d-Phe-l-Phe-Gly (fusion inhibitor peptide; FIP) conjugated to a lipidated MeV F C-term...
#1Cyrille Mathieu (French Institute of Health and Medical Research)H-Index: 18
#2Nascimento Figueira T (CUMC: Columbia University Medical Center)
Last. Matteo Porotto (CUMC: Columbia University Medical Center)H-Index: 29
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Measles virus (MeV) viral entry is mediated by a fusion complex comprised of a receptor-binding protein (hemagglutinin, H) and a fusion protein (F). The wild-type H/F complex requires interaction with specific proteinaceous receptors (CD150/SLAM and nectin-4) in order to be activated. In contrast, the H/F complexes isolated from viruses infecting the central nervous system (CNS) do not require a specific receptor. A single amino acid change in the F protein (L454W) was previously identified in t...
#1Cyrille Mathieu (Columbia University)H-Index: 18
#2Francesca T. Bovier (Columbia University)H-Index: 6
Last. Matteo Porotto (Columbia University)H-Index: 29
view all 19 authors...
Measles virus (MeV) bearing a single amino acid change in the fusion protein (F)-L454W-was isolated from two patients who died of MeV central nervous system (CNS) infection. This mutation in F confers an advantage over wild-type virus in the CNS, contributing to disease in these patients. Using murine ex vivo organotypic brain cultures and human induced pluripotent stem cell-derived brain organoids, we show that CNS adaptive mutations in F enhance the spread of virus ex vivo. The spread of virus...
#1Cyrille Mathieu (Columbia University)H-Index: 18
#1Cyrille Mathieu (Columbia University)
Last. Matteo Porotto (Columbia University)H-Index: 29
view all 14 authors...
#1Victor K. Outlaw (UW: University of Wisconsin-Madison)H-Index: 6
#2Ross W. Cheloha (UW: University of Wisconsin-Madison)H-Index: 9
Last. Anne MosconaH-Index: 42
view all 11 authors...
The lower respiratory tract infections affecting children worldwide are in large part caused by the parainfluenza viruses (HPIVs), particularly HPIV3, along with human metapneumovirus and respiratory syncytial virus, enveloped negative-strand RNA viruses. There are no vaccines for these important human pathogens, and existing treatments have limited or no efficacy. Infection by HPIV is initiated by viral glycoprotein-mediated fusion between viral and host cell membranes. A viral fusion protein (...
#2Hsiao-Yun Liu (CUMC: Columbia University Medical Center)H-Index: 1
Last. Hans-Willem Snoeck (CUMC: Columbia University Medical Center)H-Index: 29
view all 6 authors...
Lung and airway epithelial cells generated in vitro from human pluripotent stem cells (hPSCs) have applications in regenerative medicine, modeling of lung disease, drug screening and studies of human lung development. Here, we describe a strategy for directed differentiation of hPSCs into mature lung and airway epithelial cells obtained through maturation of NKX2.1+ hPSC-derived lung progenitors in a 3D matrix of collagen I in the absence of glycogen synthase kinase 3 inhibition. This protocol i...
3 CitationsSource
#1Rory D. de Vries (EUR: Erasmus University Rotterdam)H-Index: 28
#2Katharina S. Schmitz (EUR: Erasmus University Rotterdam)H-Index: 5
Last. Matteo Porotto (Columbia University)H-Index: 2
view all 19 authors...
Containment of the COVID-19 pandemic requires reducing viral transmission. SARS-CoV-2 infection is initiated by membrane fusion between the viral and host cell membranes, mediated by the viral spike protein. We have designed lipopeptide fusion inhibitors that block this critical first step of infection, and based on in vitro efficacy and in vivo biodistribution selected a dimeric form for evaluation in an animal model. Daily intranasal administration to ferrets completely prevented SARS-CoV-2 di...
32 CitationsSource
#1Stuart P. Weisberg (Columbia University)H-Index: 17
#2Thomas J. Connors (Columbia University)H-Index: 10
Last. Donna L. Farber (Columbia University)H-Index: 65
view all 31 authors...
Clinical manifestations of COVID-19 caused by the new coronavirus SARS-CoV-2 are associated with age1,2. Adults develop respiratory symptoms, which can progress to acute respiratory distress syndrome (ARDS) in the most severe form, while children are largely spared from respiratory illness but can develop a life-threatening multisystem inflammatory syndrome (MIS-C)3-5. Here, we show distinct antibody responses in children and adults after SARS-CoV-2 infection. Adult COVID-19 cohorts had anti-spi...
121 CitationsSource
Introduction: Nipah virus (NiV) is a recently emerged zoonotic paramyxovirus, capable of inter-human transmission and listed byWHO among the top eight emerging pathogens, based on the probability of causing severe outbreaks and a pandemic potential. In humans, NiV induces acute respiratory distress and encephalitis with a lethality of 40-100%.Anovel antiviral approach, based on peptideswhich interferewith the fusion of NiV with host cells has been recently developed. Research hypothesis: The pro...