Ermelindo C. Leal
University of Coimbra
Internal medicineEndocrinologyPathologyProinflammatory cytokineChemistryIn vivoOccludinOxidative stressInflammationDiabetic footBlood–retinal barrierDiabetic retinopathyWound healingAngiogenesisRetinalNitric oxideDiabetes mellitusMedicineBiologyCell biologyPharmacology
53Publications
22H-index
1,617Citations
Publications 52
Newest
#1Michelle Vang Mouritzen (RU: Roskilde University)H-Index: 4
#2Marija Petkovic (UC: University of Coimbra)H-Index: 1
Last. Håvard Jenssen (RU: Roskilde University)H-Index: 40
view all 10 authors...
Abstract Bovine lactoferricin (LFcinB) has antimicrobial and immunomodulatory properties, however, the effects on diabetic wound healing remain poorly understood. The wound healing potential of LFcinB was investigated using in vitro, ex vivo, and in vivo models. Cell migration and proliferation were tested on keratinocytes and on porcine ears. A type 1 diabetic mouse model was also used to evaluate wound healing kinetics, bacterial diversity patterns and the effect of LFcinB on oxidative stress,...
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Last. Joana Simões-Correia (UC: University of Coimbra)H-Index: 12
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Extracellular vesicles (EV) are a promising therapeutic tool in regenerative medicine. These particles were shown to accelerate wound healing, through delivery of regenerative mediators, such as microRNAs. Herein we describe an optimized and upscalable process for the isolation of EV smaller than 200 nm (sEV), secreted by umbilical cord blood mononuclear cells (UCB-MNC) under ischemic conditions and propose quality control thresholds for the isolated vesicles, based on the thorough characterizat...
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#1Marija Petkovic (UC: University of Coimbra)H-Index: 1
Last. Louise Torp DalgaardH-Index: 25
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Wound healing is a complex biological process that is impaired under diabetes conditions. Chronic non-healing wounds in diabetes are some of the most expensive healthcare expenditures worldwide. Early diagnosis and efficacious treatment strategies are needed. microRNAs (miRNAs), a class of 18–25 nucleotide long RNAs, are important regulatory molecules involved in gene expression regulation and in the repression of translation, controlling protein expression in health and disease. Recently, miRNA...
1 CitationsSource
#1Ana Figueiredo (UC: University of Coimbra)H-Index: 3
#1Ana Elizabeth Figueiredo (UC: University of Coimbra)H-Index: 18
Last. Eugenia Carvalho (UC: University of Coimbra)H-Index: 34
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Abstract Non-healing diabetic foot ulcers (DFUs) are a serious complication in diabetic patients. Their incidence has increased in recent years. Although there are several treatments for DFUs, they are often not effective enough to avoid amputation. Protein tyrosine phosphatase 1B (PTP1B) is expressed in most tissues and is a negative regulator of important metabolic pathways. PTP1B is overexpressed in tissues under diabetic conditions. Recently, PTP1B inhibition has been found to enhance wound ...
4 CitationsSource
#1Patricia Monteiro Seraphim (UNESP: Sao Paulo State University)H-Index: 12
#2Ermelindo C. Leal (UC: University of Coimbra)H-Index: 22
Last. Eugenia CarvalhoH-Index: 34
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Abstract AIMS: This study aimed to investigate the effect of lymphocytes in wound healing and the underlying mechanisms, in diabetic and non-diabetic mice, using Balb/c recombination activating gene (Rag)-2 and interleukin 2 receptor gamma (IL-2Rγ) double knockout (KO) (RAG2−/− IL-2Rγ−/−) mice. MAIN METHODS: Wound healing in vivo was performed in control and STZ-induced diabetic mice, in both KO and WT mice. Inflammation and ROS production were evaluated by immunofluorescence microscopy analysis...
2 CitationsSource
#1Ana FigueiredoH-Index: 3
Last. Eugenia CarvalhoH-Index: 34
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Peripheral neuropathy plays a role in chronic diabetic foot ulcers (DFUs), also leading to an imbalance in inflammation, as pro-inflammatory macrophages (M1) have difficulty switching to an anti-inflammatory phenotype (M2) contributing to a chronic inflammatory phase and healing impairment. Protein tyrosine phosphatase 1B (PTP1B), in the skin, is over expressed in diabetes and DFUs. We aimed to evaluate the role of PTP1B inhibition in macrophage under high glucose conditions. THP-1 monocytic cel...
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#1Ermelindo C. LealH-Index: 22
#2Ana FigueiredoH-Index: 3
Last. Eugenia CarvalhoH-Index: 34
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Diabetic neuropathy plays an important role in the development of chronic wounds. Endothelial progenitor cells (EPCs) are known important regulators of wound angiogenesis. However, the role of neuropeptides in EPC function, and particularly Neurotensin (NT), is unknown in diabetic wound healing. EPCs isolated from normal mice were cultured in high glucose (25 mM) or normoglicemia (5.5 mM) and treated with NT (25-400 nM) for 3 days. Moreover, EPCs isolated from streptozotocin (STZ)-induced diabet...
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#1Marija Petkovic (UC: University of Coimbra)H-Index: 1
#2Ermelindo C. Leal (UC: University of Coimbra)H-Index: 22
Last. Louise Torp Dalgaard (RU: Roskilde University)H-Index: 25
view all 8 authors...
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#1Helena Henriques-Antunes (UC: University of Coimbra)H-Index: 1
#2Renato M. S. Cardoso (UC: University of Coimbra)H-Index: 6
Last. Lino Ferreira (UC: University of Coimbra)H-Index: 49
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Small extracellular vesicles (SEVs) offer a promising strategy for tissue regeneration, yet their short lifetime at the injured tissue limits their efficacy. Here, we show that kinetics of SEV delivery impacts tissue regeneration at tissue, cellular, and molecular levels. We show that multiple carefully timed applications of SEVs had superior regeneration than a single dose of the same total concentration of SEVs. Importantly, diabetic and non-diabetic wounds treated with a single time point dos...
24 CitationsSource
#1João Elói Moura (UC: University of Coimbra)H-Index: 8
#1João C.V.P. Moura (UC: University of Coimbra)H-Index: 12
Last. Eugenia Carvalho (UC: University of Coimbra)H-Index: 34
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Treatment for chronic diabetic foot ulcers is limited by the inability to simultaneously address the excessive inflammation and impaired re-epithelization and remodeling. Impaired re-epithelization leads to significantly delayed wound closure and excessive inflammation causes tissue destruction, both enhancing wound pathogen colonization. Among many differentially expressed microRNAs, miR-155 is significantly upregulated and fibroblast growth factor 7 (FGF7) mRNA (target of miR-155) and protein ...
15 CitationsSource