Jian Huang
Peking University
EndocrinologyNuclear chemistryMolecular biologyAlkaline phosphataseKinaseChemistryIn vitroCrystallizationOctacalcium phosphateOsteoclastBone mineralAmorphous calcium phosphateOsteoblastCalciumMAPK/ERK pathwayMineralization (biology)Bone resorptionCrystallographyBiologyCell biology
Publications 20
#1Cai-xia Yu (PKU: Peking University)H-Index: 2
#2Jiao Gong (PKU: Peking University)H-Index: 1
Last. Kui Wang (PKU: Peking University)H-Index: 24
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Abstract Rat calvarial osteoblasts were treated with lanthanum chloride (LaCl 3 ) to explore its effect on the mineral crystalline phase during the process of osteoblast calcification in vitro . The results confirmed that La was readily deposited in the mineral component of the matrix. Employing high-resolution transmission electron microscopy and Fourier transform infrared microspectroscopy techniques, we demonstrated that features comparable to dicalcium phosphate dihydrate (DCPD) and octacalc...
3 CitationsSource
#1Xiao-rong Chen (PKU: Peking University)H-Index: 2
#2Jing Bai (PKU: Peking University)H-Index: 1
Last. Kui Wang (PKU: Peking University)H-Index: 24
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Abstract In the present study, we demonstrated that calcium phosphate (CaP) nanoparticles formed in cell culture media were implicated in the process of high inorganic phosphate (Pi) mediated osteogenic differentiation of rat bone marrow stromal cells (BMSCs). Exposure of BMSCs in vitro to high Pi-containing media reduced alkaline phosphatase (ALP) activity and the expressions of osteoblast-specific genes. The sediments of CaP nanoparticles were observed at the cell surface and some of them were...
14 CitationsSource
#1Qun ZhangH-Index: 2
#2Yun JiangH-Index: 2
Last. Kui WangH-Index: 13
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Calcium phosphate clusters are present in both aqueous solutions and an amorphous phase during crystallization. It is a challenging task to acquire the structural information on such clusters, owing to their small size, chemical lability, and inaccessibility to most detection techniques. Here, we demonstrate the feasibility of detecting such clusters in situ by synchrotron X-ray absorption near-edge spectroscopy at calcium K-edge, a technique that is sensitive to the short-range order in calcium...
23 CitationsSource
#1Kui WangH-Index: 24
#2Jian HuangH-Index: 12
Last. Xiao-rong ChenH-Index: 2
view all 5 authors...
#1Zheng-Lai Zhang (PKU: Peking University)H-Index: 1
#2Xiao-rong Chen (PKU: Peking University)H-Index: 2
Last. Kui Wang (PKU: Peking University)H-Index: 13
view all 6 authors...
Abstract The hydroxyapatite (HAP) with variable chemical substitutions has been considered as the major component in the mineralized part of bones. Various metastable crystalline phases have been suggested as transitory precursors of HAP in bone, but there are no consensuses as to the nature of these phases and their temporal evolution. In the present study, we cultured rat calvarial osteoblasts with ascorbate and β-glycerophosphate to explore which calcium phosphate precursor phases comprise th...
10 CitationsSource
#1Lin-Wei DuH-Index: 2
#2Sha BianH-Index: 4
Last. Kui WangH-Index: 13
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Amorphous calcium phosphate often forms as a precursor phase in a solution at sufficiently high supersaturation and pH, and then transforms to the thermodynamically stable hydroxyapatite. The chemical composition, structure, and property of the amorphous phase are dependent on the structure of its composing clusters. Based on the results from the measurements of in situ Ca K-edge X-ray near-edge structure and ex situ X-ray diffraction, as well as the concomitant pH change in the reaction process...
44 CitationsSource
#1Sha BianH-Index: 4
#2Lin-Wei DuH-Index: 2
Last. Kui WangH-Index: 13
view all 9 authors...
Calcium phosphate crystallization is of importance in medicine and materials science, and aggregation frequently happens at its early stage. Herein, we report a kinetic study of crystallization in particles of aggregated nanocrystals of calcium phosphate by in situ synchrotron small-angle X-ray scattering. The particles were random fractal aggregates, having diameters of 20-60 nm and comprising structural units of 2.5 nm in dimension. With the application of a logistic model, the structural chan...
9 CitationsSource
#1Jian HuangH-Index: 12
#2Tian-Lan ZhangH-Index: 15
Last. Zheng-Lai ZhangH-Index: 1
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To assess the potential effect of lanthanides(Ln)on the prevention of prostate cancer(PCa)bone metastasis,in this study we report the influence of Ln on PCa cells induced osteoblasts and osteoclasts differentiation.We cultured osteoblast-like MC3T3-e1 cells with conditioned medium(CM)from lanthanum(La)or gadolinium(Gd)treated DU145 cells.MC3T3-e1 differentiation increased in the presence of DU145 CM treated with La,whereas Gd treatment had no effect on MC3T3-e1 differentiation.Using RT-PCR array...
#1Peng Wang (PKU: Peking University)H-Index: 7
#2Xiao‑Min Zou (PKU: Peking University)H-Index: 1
Last. Kui Wang (PKU: Peking University)H-Index: 24
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This study examined whether Gd (gadolinium) could suppress prostate cancer cell migration and prostate cancer cell-induced osteoclast differentiation. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] and colony forming assay showed that GdCl3 treatment inhibited both cell viability and colony forming ability in PC3 cells more significantly than that in DU145 cells. Annexin/PI (propidium iodide) staining showed an increase in apoptotic death of PC3 cells in the presence of G...
2 CitationsSource
#1Yan-Ling Shi (Capital Medical University)H-Index: 3
#2Li-Wen Wang (Capital Medical University)H-Index: 4
Last. Kui Wang (PKU: Peking University)H-Index: 13
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A major cellular event in vascular calcification is the phenotypic transformation of vascular smooth muscle cells (VSMCs) into osteoblast-like cells. After demonstrating that lanthanum chloride (LaCl3) suppresses hydrogen peroxide-enhanced calcification in rat calcifying vascular cells (CVCs), here we report its effect on the osteoblastic differentiation of rat VSMCs, a process leading to the formation of CVCs. Cells were isolated from aortic media of male SD rats, and passages between three and...
15 CitationsSource
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