Sara N. Richter
University of Padua
PromoterGeneGenomeTopoisomeraseMolecular biologyVirologyChemistryLong terminal repeatCombinatorial chemistryNucleic acidVirusTranscription (biology)GeneticsDNABiochemistryRNAComputational biologyStereochemistryG-quadruplexBiologyMicrobiologyCell biology
111Publications
33H-index
2,964Citations
Publications 110
Newest
#1Sara Lago (UNIPD: University of Padua)H-Index: 7
#2Matteo Nadai (UNIPD: University of Padua)H-Index: 20
Last. Sara N. Richter (UNIPD: University of Padua)H-Index: 33
view all 0 authors...
Cell identity is maintained by activation of cell-specific gene programs, regulated by epigenetic marks, transcription factors and chromatin organization. DNA G-quadruplex (G4)-folded regions in cells were reported to be associated with either increased or decreased transcriptional activity. By G4-ChIP-seq/RNA-seq analysis on liposarcoma cells we confirmed that G4s in promoters are invariably associated with high transcription levels in open chromatin. Comparing G4 presence, location and transcr...
1 CitationsSource
#1Ilaria Frasson (UNIPD: University of Padua)H-Index: 16
#2Paola Soldà (UNIPD: University of Padua)H-Index: 1
Last. Sara N. Richter (UNIPD: University of Padua)H-Index: 33
view all 5 authors...
G-quadruplexes (G4s) are four-stranded nucleic acid structures abundant at gene promoters. They can adopt several distinctive conformations. G4s have been shown to form in the herpes simplex virus-1 (HSV-1) genome during its viral cycle. Here by cross-linking/pull-down assay we identified ICP4, the major HSV-1 transcription factor, as the protein that most efficiently interacts with viral G4s during infection. ICP4 specific and direct binding and unfolding of parallel G4s, including those presen...
Source
#1Martina Tassinari (University of Milan)
#2Sara N. Richter (UNIPD: University of Padua)H-Index: 33
Last. Paolo Gandellini (University of Milan)H-Index: 25
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Noncoding RNAs are functional transcripts that are not translated into proteins. They represent the largest portion of the human transcriptome and have been shown to regulate gene expression networks in both physiological and pathological cell conditions. Research in this field has made remarkable progress in the comprehension of how aberrations in noncoding RNA drive relevant disease-associated phenotypes; however, the biological role and mechanism of action of several noncoding RNAs still need...
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#1Sara ArtusiH-Index: 4
#2Emanuela RuggieroH-Index: 9
Last. Sara N. RichterH-Index: 33
view all 10 authors...
The herpes simplex virus 1 (HSV-1) genome is extremely rich in guanine tracts that fold into G-quadruplexes (G4s), nucleic acid secondary structures implicated in key biological functions. Viral G4s were visualized in HSV-1 infected cells, with massive virus cycle-dependent G4-formation peaking during viral DNA replication. Small molecules that specifically interact with G4s have been shown to inhibit HSV-1 DNA replication. We here investigated the antiviral activity of TMPyP4, a porphyrin known...
1 CitationsSource
#1Sara Lago (UNIPD: University of Padua)H-Index: 7
#2Matteo Nadai (UNIPD: University of Padua)H-Index: 20
Last. Sara N. Richter (UNIPD: University of Padua)H-Index: 33
view all 13 authors...
Well-differentiated liposarcoma (WDLPS) is a malignant neoplasia hard to diagnose and treat. Its main molecular signature is amplification of the MDM2-containing genomic region. The MDM2 oncogene is the master regulator of p53: its overexpression enhances p53 degradation and inhibits apoptosis, leading to the tumoral phenotype. Here, we show that the MDM2 inducible promoter G-rich region folds into stable G-quadruplexes both in vitro and in vivo and it is specifically recognized by cellular heli...
2 CitationsSource
Abstract Here we report on the design, preparation and investigation of four analogues of the anti-HIV G-quadruplex-forming Hotoda’s aptamer, based on an unprecedented linear topology. In these derivatives, four TGGGAGT tracts have been joined together by exploiting 3′-3′ and 5′-5′ inversion of polarity sites formed by canonical phosphodiester bonds or a glycerol-based linker. Circular dichroism data suggest that all oligodeoxynucleotides fold in monomolecular G-quadruplex structures characteriz...
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#1S. Lago (LMU: Ludwig Maximilian University of Munich)H-Index: 1
#2Filippo M. Cernilogar (LMU: Ludwig Maximilian University of Munich)H-Index: 13
Last. Sara N. Richter (UNIPD: University of Padua)H-Index: 33
view all 7 authors...
Abstract Cell identity is maintained by activation of cell-specific gene programs, regulated by epigenetic marks, transcription factors and chromatin organization1-3. DNA G-quadruplex (G4)-folded regions in cells were reported to be associated with either increased or decreased transcriptional activity4,5. By G4 ChIP-seq/RNA-seq analysis on liposarcoma cells we confirmed that G4s in promoters are invariably associated with high transcription levels in open chromatin. Comparing G4 presence, locat...
1 CitationsSource
#1Ilaria Frasson (UNIPD: University of Padua)H-Index: 16
#2Paola Soldà (UNIPD: University of Padua)H-Index: 1
Last. Sara N. Richter (UNIPD: University of Padua)H-Index: 33
view all 5 authors...
G-quadruplexes (G4s), four-stranded nucleic acid structures that adopt several distinctive conformations, are abundant at gene promoters and have been proposed as transcription regulatory elements. G4s form in the herpes simplex virus-1 (HSV-1) genome during its viral cycle. Here by cross-linking/pull-down assay we identified ICP4 as the protein that most interacts with viral G4s during infection. In vitro and in infected cells, ICP4 specifically and directly bound and unfolded parallel G4s, inc...
1 CitationsSource
#1Martina Tassinari (UNIPD: University of Padua)H-Index: 6
#2Michela Zuffo (UNIPV: University of Pavia)H-Index: 8
Last. Sara N. Richter (UNIPD: University of Padua)H-Index: 33
view all 8 authors...
Targeting of G-quadruplexes, non-canonical conformations that form in G-rich regions of nucleic acids, has been proposed as a novel therapeutic strategy toward several diseases, including cancer and infections. The unavailability of highly selective molecules targeting a G-quadruplex of choice has hampered relevant applications. Herein, we describe a novel approach, based on naphthalene diimide (NDI)-peptide nucleic acid (PNA) conjugates, taking advantage of the cooperative interaction of the ND...
7 CitationsSource
#1Annalisa FerinoH-Index: 4
#2Giulia NicolettoH-Index: 1
Last. Luigi E. XodoH-Index: 32
view all 9 authors...
Designing small molecules capable to break down G4 structures in mRNA (RG4s) offers an interesting approach to cancer therapy. Here, we have studied cationic porphyrins (CPs) bearing an alkyl chain up to 12 carbons, as they bind to RG4s while generating reactive oxygen species upon photoirradiation. FACS and confocal microscopy showed that the designed alkyl CPs strongly penetrate cell membranes, binding to KRAS and NRAS mRNAs under low-abundance cell conditions. In Panc-1 cells, alkyl CPs promo...
9 CitationsSource