Tetsuya Kishi
Nagoya University
TransthyretinInternal medicineEndocrinologyBlood proteinsMolecular biologyChemistryTransferrinCaseinLeucineIsoleucineValineRetinolWeight gainUreaAmino acid infusionInfusion solutionTotal amino acidsNitrogen sourcePlasma levelsFood intakeFood scienceAmino acidNitrogenBiochemistryParenteral nutritionNitrogen balanceBiology
18Publications
6H-index
163Citations
Publications 18
Newest
#1Tohru Ogawa (Nagoya University)H-Index: 20
#2Yoshitaka Yamada (Nagoya University)H-Index: 2
Last. Shigeki Moriya (Nara Institute of Science and Technology)H-Index: 23
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Summary Replication of the Escherichia coli chromosome is initiated synchronously from all origins (oriC) present in a cell at a fixed time in the cell cycle under given steady state culture conditions. A mechanism to ensure the cyclic initiation events operates through the chromosomal site, datA, which titrates exceptionally large amounts of the bacterial initiator protein, DnaA, to prevent overinitiation. Deletion of the datA locus results in extra initiations and altered temporal control of r...
71 CitationsSource
Plasma transferrin (Tf), transthyretin (TTR), and retinol-binding protein (RBP) have been proposed to be sensitive nutritional parameters. We investigated the effect of laparotomy and/or starvation on these plasma protein levels in rats. Starvation decreased the Tf, TTR, and RBP levels to 62.2%, 41.2%, and 52.0% of the initial values, respectively, until day 3, but then sustained the day-3 levels on day 4. In the starved and laparotomized rats, the TTR level on day 1 and the RBP level on day 2 w...
1 CitationsSource
Rapid-turnover plasma proteins (transferrin, transthyretin, and retinol-binding protein) have been proposed to be sensitive nutritional parameters. In this study, we investigated the daily changes in the rapid-turnover protein levels of rats whose nutritional conditions were controlled by parenteral nutrition under the acute-phase response. We measured the plasma fibrinogen level to monitor the acute-phase response, and the level shows that the acute-phase response was induced by the surgical tr...
1 CitationsSource
A sandwich enzyme immunoassay for rat transthyretin using a recombinant antigen approach is described. Rat transthyretin cDNA was cloned by PCR. Rat transthyretin fused with the IgG-binding ZZ domain of protein A was expressed in E. coli using the fusion vector pEZZ18. Antibody against the fusion protein was specific to rat transthyretin in plasma as shown by immunoblotting. Affinity-purified anti-rat transthyretin-ZZ was biotinylated and used for the sandwich enzyme immunoassay. In this assay, ...
5 CitationsSource
In this study, we investigated the optimal ratio of individual branched-chain amino acids (BCAA) in a balanced amino acid infusion in laparotomized rats. The total BCAA contents of four amino acid infusions were fixed at 31% of total amino acids. The weight ratios of individual BCAA (isoleucine:leucine:valine) in the solutions were 1:0.5:1, 1:1:1, 1:2:1, and 1:4:1, respectively. The laparotomized rats were infused with about 140 mg (experiment 1) and 100 mg (experiment 2) of nitrogen and 10 g of...
7 CitationsSource
#1Yasuo IwasawaH-Index: 4
#2Tetsuya KishiH-Index: 6
Last. Tadashi SatoH-Index: 3
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Daily changes of nutritional status during total parenteral nutrition (TPN) were studied in partially hepatectomized (PH) rats. When PH rats were parenterally infused with 8 to 10 g of amino acids and 40 to 60 g of glucose per kg daily for 1 to 3 days, the nitrogen balances in PH rats were positive during the infusion period, but the balance was significantly lower on day 3 than that in nonhepatectomized (NPH) rats. Urinary total catecholamine levels in PH rats tended to be higher (45 to 70%) th...
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Abstract The development of a sandwich enzyme immunoassay for rat transferrin with two monoclonal antibodies is described. Microtiter plates coated with one monoclonal antibody (15C2H3) were used, and captured transferrin was estimated with a horseradish peroxidase-conjugated Fab' fragment of another monoclonal antibody (22A06D2). In this assay, the measurable range is 5–150 ng/ml and the coefficients of variation within and between the assay series are 1.2–5.0 and 3.3–6.0%, respectively. Recove...
3 CitationsSource
In this study, we investigated the optimal contents of branched-chain amino acids (BCAA) in a balanced amino acid infusion in laparotomized rats. The BCAA contents of four infusion solutions used were prepared to 22, 31, 40, and 49% of total amino acids, respectively. The amounts of essential amino acids except for BCAA were equal in all the solutions. Rats weighing about 240 g were infused with about 200 mg of nitrogen and 10 g of glucose daily for 7 days and evaluated for body weight change, n...
1 CitationsSource
Two experiments were conducted with male rats weighing 170 to 190 grams. In experiment 1, some nutritional parameters were determined in tumor-bearing (TB) (Walker 256 carcinosarcoma) rats fed a 23.6% casein diet for 4 weeks after the tumor inoculation. Cumulative weight gain and food intake were less in TB rats than in nontumor-bearing (NTB) rats. At 3 and 4 weeks after the tumor inoculation, plasma histidine, alanine, and glycine levels were higher in TB rats than in NTB animals. The arginine ...
23 CitationsSource
Nutritional effects of intravenous infusion of an amino acid (AA) mixture enriched with the branched chain AA were previously evaluated at a daily level of 45 kcal and 200 mg N using male rats weighing approximately 200 g. The present study was conducted with male rats weighing approximately 200 g to evaluate the nutritional effects of 1) an AA infusion solution at further increased energy level, and 2) an AA solution devoid of aspartic and glutamic acids. By increasing daily energy input from 4...
1 CitationsSource