Dongfeng Chen
Guangzhou University of Chinese Medicine
AdductInternal medicineEndocrinologyCellMolecular biologyChemistryApoptosisMesenchymal stem cellHydroxyl radicalAntioxidantMTT assayABTSDPPHStem cellElectrospray ionizationChromatographyBiochemistrySignal transductionStereochemistryMedicineBiologyCell biologyPharmacology
130Publications
22H-index
1,670Citations
Publications 133
Newest
#1Hao Pan (Guangzhou University of Chinese Medicine)H-Index: 2
#2Yuqing Lin (Guangzhou University of Chinese Medicine)H-Index: 1
Last. Dongfeng Chen (Guangzhou University of Chinese Medicine)H-Index: 22
view all 12 authors...
OBJECTIVES Wedelolactone exhibits regulatory effects on some inflammatory diseases. However, the anti-inflammatory mechanism of wedelolactone has not been entirely unravelled. Therefore, the present study focuses on investigating the mechanism of wedelolactone on NLRP3 inflammasome in macrophages and its influence on MSU-induced inflammation. MATERIALS AND METHODS BMDM, J774A.1 and PMA-differentiated THP-1 macrophages were primed with LPS and then stimulated with ATP or nigericin or MSU crystal ...
9 CitationsSource
#1Hang Li (Guangzhou University of Chinese Medicine)H-Index: 3
#2Caiping Zhao (Guangzhou University of Chinese Medicine)H-Index: 3
Last. Meiling ZhuH-Index: 4
view all 10 authors...
Abstract Src family kinases (SFKs) is a non-receptor protein tyrosine kinases family. They are crucial in signal transduction and regulation of various cell biological processes, such as proliferation, differentiation and apoptosis. The role and mechanism of SFKs in tumorigenesis have been widely studied. However, more and more studies have also shown that SFKs are involved in the pathogenesis of pulmonary fibrosis (PF). Myofibroblasts activation, epithelial-mesenchymal transition and inflammati...
8 CitationsSource
#1Wenwen Si (Guangzhou University of Chinese Medicine)H-Index: 4
#2Yi Li (Guangzhou University of Chinese Medicine)H-Index: 2
Last. Meiling ZhuH-Index: 4
view all 8 authors...
The modification of methyltransferase-like (METTL) enzymes plays important roles in various cellular responses by regulating microRNA expression. However, how m6A modification is involved in stress granule (SG) formation in the early stage of acute ischemic stroke by affecting the biogenesis processing of microRNAs remains unclear. Here, we established a middle cerebral artery occlusion (MCAO) model in rats and an oxygen-glucose deprivation/reperfusion (OGD/R) model in primary cortical neurons a...
6 CitationsSource
#1Quan Wen (Guangzhou University of Chinese Medicine)H-Index: 2
#2Jifei Miao (Guangzhou University of Chinese Medicine)H-Index: 5
Last. Hui Li (Guangzhou University of Chinese Medicine)H-Index: 2
view all 14 authors...
Inflammation is a common inducer of numerous severe diseases such as sepsis. The NF-κB signaling pathway plays a key role in the inflammatory process. Its activation promotes the release of pro-inf...
3 CitationsSource
#1Yamei Liu (Guangzhou University of Chinese Medicine)H-Index: 9
#2Liangliang Xu (Guangzhou University of Chinese Medicine)H-Index: 22
Last. Gang Li (CUHK: The Chinese University of Hong Kong)H-Index: 84
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Abstract Mesenchymal stem cells (MSCs) can be easily expanded without losing the ability of multilineage differentiation, including oesteogenic, chondrogenic and adipogenic differentiation. These characters make MSCs a promising cell resource for cartilage defect repair. MSCs could be recruited by inflammatory stimulation, then home to the injury tissues. However, its capacity of homing is extremely limited. Thus, it has become extremely necessary to develop an agent or a method, which can be us...
2 CitationsSource
#1Jun Zhong (Guangzhou University of Chinese Medicine)H-Index: 1
#2Sen Ye (Guangzhou University of Chinese Medicine)H-Index: 2
Last. Caixia Li (Guangzhou University of Chinese Medicine)
view all 9 authors...
In recent years, stem cells have gained much attention for the treatment of neurodegenerative diseases. However, inducing neural stem cell directionally differentiation is a difficult problem in the treatment of Parkinson's disease (PD) by stem cell therapy. Plastrum Testudinis (PT) can enhance the number of TH-positive neurons in the PD rat brain substantia nigra, but the underlying mechanism has not been clarified. Here, we aimed at further investigating the mechanism by which PT can promote N...
Source
#1Li Na Zhou (Guangzhou University of Chinese Medicine)H-Index: 1
#2Jia Chuan Wang (Guangzhou University of Chinese Medicine)H-Index: 1
Last. Chen Df (Guangzhou University of Chinese Medicine)
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BACKGROUND: To date, it has repeatedly been demonstrated that infusing bone marrow-derived stem cells (BMSCs) into acellular nerve scaffolds can promote and support axon regeneration through a peripheral nerve defect. However, harvesting BMSCs is an invasive and painful process fraught with a low cellular yield. METHODS: In pursuit of alternative stem cell sources, we isolated stem cells from the inguinal subcutaneous adipose tissue of adult Sprague-Dawley rats (adipose-derived stem cells, ADSCs...
7 CitationsSource
#1Xican Li (Guangzhou University of Chinese Medicine)H-Index: 19
#2Jingyuan Zeng (Guangzhou University of Chinese Medicine)H-Index: 1
Last. Dongfeng Chen (Guangzhou University of Chinese Medicine)H-Index: 22
view all 8 authors...
In this study, the anti-ferroptosis effects of catecholic flavonol quercetin and its metabolite quercetin Diels-Alder anti-dimer (QDAD) were studied using an erastin-treated bone marrow-derived mesenchymal stem cell (bmMSCs) model. Quercetin exhibited higher anti-ferroptosis levels than QDAD, as indicated by 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid (C11-BODIPY), 2′,7′-dichlorodihydrofluoroscein diacetate (H2DCFDA), lactate dehydrogenase (LDH) relea...
12 CitationsSource
#1Jie Liu (Guangzhou University of Chinese Medicine)H-Index: 3
#2Xican Li (Guangzhou University of Chinese Medicine)H-Index: 19
Last. Dongfeng Chen (Guangzhou University of Chinese Medicine)H-Index: 22
view all 7 authors...
To elucidate the mechanism of anti-ferroptosis and examine structural optimization in natural phenolics, cellular and chemical assays were performed with 2′-hydroxy chalcone butein and dihydroflavone (S)-butin. C11-BODIPY staining and flow cytometric assays suggest that butein more effectively inhibits ferroptosis in erastin-treated bone marrow-derived mesenchymal stem cells than (S)-butin. Butein also exhibited higher antioxidant percentages than (S)-butin in five antioxidant assays: linoleic a...
10 CitationsSource
#1Xican Li (Guangzhou University of Chinese Medicine)H-Index: 19
#2Tingting Wang (Guangzhou University of Chinese Medicine)H-Index: 13
Last. Dongfeng Chen (Guangzhou University of Chinese Medicine)H-Index: 22
view all 8 authors...
Abstract The antioxidant properties of coumestans have been investigated previously using inappropriate methods, which has resulted in misleading and inaccurate mechanisms being reported to account for their antioxidant behavior. In this study, the phenolic coumestan wedelolactone increased the viability of OH -treated mesenchymal stem cells (MSCs) in a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl assay. Mechanistic analysis in vitro suggested that wedelolactone could scavenge various radicals, inc...
20 CitationsSource