Chengye Che
Qingdao University
OphthalmologyInnate immune systemMolecular biologyProinflammatory cytokineTumor necrosis factor alphaChemistryImmunologyMyeloperoxidaseFungal keratitisWestern blotCorneaInflammationVisual acuityKeratitisCytokineCorneal epitheliumAspergillus fumigatusMedicineBiologyMicrobiology
60Publications
15H-index
458Citations
Publications 60
Newest
Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), led to an outbreak of viral pneumonia in December 2019 null The present study aimed to investigate the host inflammatory response signature-caused by SARS-CoV-2 in human corneal epithelial cells (HCECs) null The expression level of angiotensin-converting enzyme 2 (ACE2) in the human cornea was determined via immunofluorescence null In vitro experiments were performed in HCECs stimulat...
1 CitationsSource
#1Guoqiang Zhu (Jining Medical University)H-Index: 7
#2Leyu Lyu (Qingdao University)H-Index: 1
Last. Chengye Che (Qingdao University)H-Index: 15
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Purpose C-type lectin-like receptor-1 (CLEC-1) is a member of the Dectin-1 cluster of pattern recognition receptors (PRRs). It is involved in host immunity, has immunoregulatory function, and supports allograft tolerance. Our study aimed to describe the role of CLEC-1 in response to fungal keratitis, in situ, in vivo, and in vitro. Methods Quantitative polymerase chain reaction (qRT-PCR) and immunofluorescence were used to detect the expression of CLEC-1 in corneas of patients with Aspergillus f...
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#1Haijing Yan (Qingdao University)H-Index: 1
#2Nan JiangH-Index: 13
Last. Chengye CheH-Index: 15
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#1Wenyi Zhao (Qingdao University)H-Index: 3
#2Hua Yang (Qingdao University)H-Index: 2
Last. Chengye Che (Qingdao University)H-Index: 15
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The protein GSDMD is an important performer of pyroptosis and a universal substrate for the inflammatory caspase. However, the role and regulatory mechanism of GSDMD in Aspergillus fumigatus keratitis is remains unknown. Here we detected GSDMD protein in the cornea of normal and fungal-infected C57BL/6 mice. Human corneal epithelial cell (HCECs) were preincubated with a hydrochloride solution (IFNR inhibitor), ruxolitinib (JAK/STAT inhibitor), belnacasan (caspase-1 inhibitor) before infection wi...
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#1Su Hwan Park (PNU: Pusan National University)H-Index: 2
#2Chengye Che (Qingdao University)H-Index: 15
Last. Ji Eun Lee (PNU: Pusan National University)H-Index: 19
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Aim To compare the clinical results of femtosecond (FS) laser in situ keratomileusis (LASIK) in high myopic patients and low myopic patients. Methods This study included 212 myopic eyes undergoing LASIK using a VisuMax 500kHz FS laser. All treated eyes were assigned to one of two groups according to preoperative manifest spherical refraction: low myopia group (A, >-4.0 D) and high myopia group (B, ≤-4.0 D). Uncorrected and corrected distance visual acuity (UDVA, CDVA), refractive errors, and hig...
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#1Leyu Lyu (Qingdao University)H-Index: 1
#2Li-Ting Hu (Qingdao University)H-Index: 13
Last. Chengye Che (Qingdao University)H-Index: 15
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Abstract Aim To clarify the regulatory mechanisms of lacrimal androgen-binding proteins (ABPs) in mice with keratitis caused by Aspergillus fumigatus (A. fumigatus). Methods Mouse models of A. fumigatus keratitis were established. Lacrimal glands were removed after 24 h for general and histological comparison. Lacrimal ABPs were detected by qRT-PCR and quantitative proteomic analysis, or were detected by qRT-PCR after subconjunctival or lacrimal gland injection with dexamethasone. Unique inflamm...
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#1Xing Liu (Qingdao University)H-Index: 1
#2Jia You (Qingdao University)H-Index: 2
Last. Jing Lin (Qingdao University)H-Index: 14
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Mammalian Ste20-like kinase 4 (MST4), a new member of the germinal-center kinase STE20 family, was recently demonstrated to be a negative regulator of inflammation. However, whether MST4 participates in the inflammatory response to fungal infection remains unknown. Our study investigated the role and molecular mechanisms of MST4 in mice cornea and corneal epithelial cells exposed to Aspergillus fumigatus (A. fumigatus). Protein level of MST4 was detected in mice corneas and human corneal epithel...
2 CitationsSource
#1Meng-Qi Wu (Qingdao University)H-Index: 1
#2Cui Li (Qingdao University)H-Index: 13
Last. Guiqiu Zhao (Qingdao University)H-Index: 17
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AIM: To determine the roles of high-mobility group box1 (HMGB1) in pro-inflammation, host immune response and its potential target for treatment in Aspergillus fumigatus (A.fumigatus) keratitis. METHODS: Expression of HMGB1 was tested in C57BL/6 normal and infected corneas. Dual immunostaining tested co-expression of HMGB1 with TLR4 or LOX-1. C57BL/6 mice were pretreated with Box A or PBS and then infected. Clinical scores, polymerase chain reaction, ELISA, and MPO assay were used to assess the ...
2 CitationsSource
#1Leyu Lyu (Qingdao University)H-Index: 1
#2Qian Wang (Qingdao University)H-Index: 14
Last. Chengye Che (Qingdao University)H-Index: 15
view all 6 authors...
AIM: To investigate the expression of lacrimal androgen-binding proteins (ABPs) in mice Pseudomonas aeruginosa (P. aeruginosa) keratitis. METHODS: P. aeruginosa mice model from different gender was developed by intra-stromal injection. The expression of lacrimal ABPs in lacrimal gland specimens from P. aeruginosa keratitis mice was detected by the quantitative polymerase chain reaction (qRT-PCR). Corneal virulence was evaluated based on clinical scores. To study the mechanism of lacrimal ABPs' e...
1 CitationsSource
#1Hua Yang (Qingdao University)H-Index: 2
#2Qian Wang (Qingdao University)H-Index: 14
Last. Chengye Che (Qingdao University)H-Index: 15
view all 9 authors...
Abstract Purpose Nerolidol, a naturally occurring sesquiterpene has both anti-microbial and anti-inflammatory properties. The current study aims to investigate the antifungal and the anti-inflammatory effects of nerolidol against mouse Aspergillus fumigatus (A. fumigatus) keratitis. Methods The minimum inhibitory concentration (MIC) and cytotoxicity tests were used to study the antifungal ability. For in vivo and in vitro studies, the mouse corneas and the human corneal epithelial cells (HCECs) ...
3 CitationsSource