Samanta Paltrinieri
University of Bologna
GeneIdentification (biology)HorticultureNested polymerase chain reactionVirologyRestriction fragment length polymorphismCultivarRibosomal DNABroomPhytoplasmaAster yellowsMolecular identificationTuf gene16S ribosomal RNAVeterinary medicinePolymerase chain reactionGeneticsBotanyRibosomal RNABiology
156Publications
16H-index
1,017Citations
Publications 108
Newest
#1Manuel Joaquim Oliveira (UCP: Catholic University of Portugal)H-Index: 2
#2Sandra Sousa e Castro (University of Porto)H-Index: 2
Last. Susana M.P. Carvalho (University of Porto)H-Index: 17
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Abstract “Flavescence doree” (FD) is a quarantine disease associated with the presence of a phytoplasma transmitted by the insect vector Scaphoideus titanus. This disease affects grapevines and is of a great concern to the stability and sustainability of the wine industry, due to the harvest losses and death of infected plants. In Portugal, FD has seriously affected the “Vinhos Verdes” region, but so far the research on this topic is very limited. The current study confirmed that the FD phytopla...
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To verify the risk that plant pathogens such as phytoplasmas could have been introduced with infected propagation material, a preliminary investigation for the presence of phytoplasmas and their insect vectors in the main Tunisian viticultural regions was carried out. To identify phytoplasmas and leafhopper fauna several survey have been carried out on grapevine and spontaneous plant species in the main grapevine growing regions of Tunisia, i.e. Rafraf, Baddar, Mraissa, Belli, Gobba, and Bousale...
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#1Nicolás QuirogaH-Index: 5
#2V. LongoneH-Index: 1
Last. Nicola FioreH-Index: 11
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#1Nicoletta Contaldo (UNIBO: University of Bologna)H-Index: 11
#2Gianfranco D’Amico (UNIBO: University of Bologna)H-Index: 2
Last. Yaima Arocha RoseteH-Index: 4
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Abstract Cote d’Ivoire lethal yellowing (CILY) is a devastating disease associated with phytoplasmas and has recently rapidly spread to several coconut-growing areas in the Country. Phytoplasmas are phloem-restricted bacteria that affect plant species worldwide. These bacteria are transmitted by plant sap-feeding insects, and their cultivation was recently achieved in complex artificial media. In this study, phytoplasmas were isolated for the first time from coconut palm trunk borings in both so...
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#1Didem Canik OrelH-Index: 2
#2Samanta PaltrinieriH-Index: 16
Last. Assunta BertacciniH-Index: 39
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Ankara ve Isparta illerinden fitoplazma enfeksiyonu benzeri belirti gosteren elma, armut ve kayisi agaclarindan fitoplazma varliginin saptanmasina yonelik ornekleme yapilmistir. Toplamda 31 ornek universal ve grup spesifik fitoplazma primerleri ile PCR’a tabi tutulmus ve elde edilen PCR urunleri Tru1 I, Rsa I and Ssp I endonukleazlari ile kesilmistir. Elma ornekleri 16SrX-A (‘ Candidatus Phytoplasma mali’) ile enfekteli bulunurken armut orneklerinin cogunlugunda 16SrX-A ve 16SrX-C alt gruplariyl...
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#1Eleonora Satta (UNIBO: University of Bologna)H-Index: 4
#2Samanta Paltrinieri (UNIBO: University of Bologna)H-Index: 16
Last. Assunta Bertaccini (UNIBO: University of Bologna)H-Index: 39
view all 3 authors...
The transmission of phytoplasmas by seed was reported in several herbaceous and some woody plant species. The seedlings were usually analyzed for the presence of phytoplasmas in different stages of growth by nested-PCR/RFLP analysis and phytoplasmas belonging to different ribosomal groups according with species and geographical distribution were detected. The phytoplasma isolation from corn seedlings confirms the seed transmission of viable phytoplasma cells. The sudden epidemic events associate...
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#1Assunta Bertaccini (UNIBO: University of Bologna)H-Index: 39
#2Samanta Paltrinieri (UNIBO: University of Bologna)H-Index: 16
Last. Nicoletta Contaldo (UNIBO: University of Bologna)H-Index: 11
view all 3 authors...
: Phytoplasma detection and identification is primarily based on PCR followed by restriction fragment length polymorphism analysis. This method detects and differentiates phytoplasmas including those not yet identified. The protocol describes the application of this method for identification of phytoplasmas at 16S rRNA (16Sr) group and 16Sr subgroup levels on amplicons and also in silico on the same sequences.
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#1Gaia CarminatiH-Index: 1
#2Eleonora SattaH-Index: 4
Last. Assunta BertacciniH-Index: 39
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Analysis on commercial carrot seeds and derived plants were performed to evaluate the ‘Candidatus Liberibacter solanacearum’ and ‘Candidatus Phytoplasma’ presence. The analyses on the seeds demonstrated the presence of both prokaryotes in nearly 100% of the seed batches tested. Sequencing confirmed that ‘Ca. L. solanacearum’ strains detected in the seed samples belong to the haplotype D. Seedlings were grown in an insect proof greenhouse and in a growing chamber under controlled conditions. Symp...
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Coconut lethal yellowing (LY) is the single most important disease presently affecting the coconut production worldwide. Symptomatic and asymptomatic coconut plants were sampled in some selected areas to verify the identity of phytoplasmas associated with LY in Cuba. Diverse phytoplasma ribosomal groups were detected in the samples from symptomatic palms such as 16SrXII, 16SrVII, and 16SrI. In several other palms 16SrIV-A subgroup phytoplasmas were identified, in the groEL gene the only positive...
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#1Samanta PaltrinieriH-Index: 16
#2Yuri ZambonH-Index: 3
Last. Nicoletta ContaldoH-Index: 11
view all 6 authors...
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