Dominic C. Chow
Duke University
Surface plasmon resonanceHaematopoiesisPharmacokineticsSoft lithographyMolecular electronicsFusion proteinBiophysicsOligonucleotideGreen fluorescent proteinProtein engineeringCellTerminal deoxynucleotidyl transferaseCompartment (development)BiosensorMonomerNanotechnologyEnzymeChemistryCell typeSynthetic biologyMaterials scienceProgenitor cellIn vitroImmunologyIn vivoEmerging technologiesNucleic acidAsparagineBiocompatibilityElastin like polypeptidesValineBiomaterialBiopolymerPeptideMegakaryocyteProlineDrug deliveryAlanineBone marrowLiaisonStem cellHematopoietic TissueOxygen tensionFabrication methodsIsotopic labelingRecombinant DNAPolymerizationBiomoleculeTissue engineeringAmino acidYield (chemistry)Escherichia coliDNABiochemistryMonolayerStereochemistryDeposition (chemistry)TemplateBiologyProtein A/GProtein purificationCell biology
10Publications
9H-index
1,028Citations
Publications 10
Newest
#1Dominic C. Chow (Duke University)H-Index: 9
#2Michelle L. Nunalee (Duke University)H-Index: 2
Last. Ashutosh Chilkoti (Duke University)H-Index: 103
view all 5 authors...
Peptides are emerging as a new class of biomaterials due to their unique chemical, physical, and biological properties. The development of peptide-based biomaterials is driven by the convergence of protein engineering and macromolecular self-assembly. This review covers the basic principles, applications, and prospects of peptide-based biomaterials. We focus on both chemically synthesized and genetically encoded peptides, including poly-amino acids, elastin-like polypeptides, silk-like polymers,...
291 CitationsSource
#1Dominic C. Chow (Duke University)H-Index: 9
#2Ashutosh ChilkotiH-Index: 103
We describe a technique to synthesize DNA homopolymers on a surface using surface-initiated enzymatic polymerization (SIEP) with terminal deoxynucleotidyl transferase (TdTase), an enzyme that repetitively adds mononucleotides to the 3‘-end of oligonucleotides. The thickness of the synthesized DNA layer was found to depend on the deoxymononucleotide monomer, in the order of dATP > dTTP ≫ dGTP ≈ dCTP. In addition, the composition and the surface density of oligonucleotide initiators were also impo...
27 CitationsSource
#1Andrew J. Simnick (Duke University)H-Index: 10
#2Dong Woo Lim (Duke University)H-Index: 29
Last. Ashutosh Chilkoti (Duke University)H-Index: 103
view all 4 authors...
Elastin‐like polypeptides (ELPs) are a unique class of genetically encoded biopolymers with tunable thermosensitivity and biocompatibility. This review provides an introduction to ELPs and an overview of their design and synthesis. It also discusses applications of these polypeptides for drug delivery, tissue engineering, protein purification, and biosensing.
114 CitationsSource
#1Stefan ZauscherH-Index: 48
#2Matthew S. JohannesH-Index: 15
Last. Robert L. ClarkH-Index: 65
view all 6 authors...
Source
#1Wenge Liu (Duke University)H-Index: 23
#2Matthew R. Dreher (Duke University)H-Index: 35
Last. Ashutosh Chilkoti (Duke University)H-Index: 103
view all 5 authors...
We report a method to incorporate a stable isotope (13C) and a radioactive isotope (14C) into a recombinant polypeptide during Escherichia coli culture in M9 minimal medium supplemented with universally labeled 13C- or 14C-labeled glucose. We chose a thermally responsive elastin-like polypeptide (ELP) as a model polypeptide for this study because of its utility in various biotechnology applications such as drug delivery and tissue engineering. High cell densities were obtained by step-wise adapt...
35 CitationsSource
#1Dominic C. Chow (Duke University)H-Index: 9
#2Matthew R. Dreher (Duke University)H-Index: 35
Last. Ashutosh Chilkoti (Duke University)H-Index: 103
view all 4 authors...
Elastin-like polypeptides (ELPs) are recombinant peptide-based biopolymers that contain repetitive sequences enriched in glycine, valine, proline, and alanine. Because of the unusually large fraction of these amino acids in ELPs as compared to other cellular proteins, we hypothesized that intracellular pools of these amino acids can be selectively depleted and limit protein yields during expression. In this study, we examined how culture conditions and individual medium components affect protein...
153 CitationsSource
#1Dominic C. Chow (Duke University)H-Index: 9
#2Matthew S. Johannes (Duke University)H-Index: 15
Last. Ashutosh Chilkoti (Duke University)H-Index: 103
view all 6 authors...
The advances in biotechnology and nanotechnology are spawning a new and exciting manufacturing tool – bionanofabrication – which enables revolutionary ways of building complex bionanostructures on surfaces with nanometer precision. Here, we highlight the opportunities and challenges in the development of this emerging technology and discuss its use in genomics, proteomics, nanostructures, nanomaterials, drug discovery, and synthetic biology. To provide useful insights into the future directions ...
23 CitationsSource
#1Dominic C. Chow (Duke University)H-Index: 9
#2Woo-Kyung LeeH-Index: 15
Last. Ashutosh Chilkoti (Duke University)H-Index: 103
view all 4 authors...
Nucleic acid nanostructures are useful as templates for bionanofabrication of composite molecular nanostructures in materials science, molecular electronics, and biosensing. Here, we demonstrate that terminal deoxynucleotidyl transferase, which repetitively adds mononucleotides to the 3‘ end of a short DNA initiator, can be used to rapidly fabricate DNA nanostructures up to 121 nm high with lateral dimensions from 0.1 to 4 μm in 2 h. These programmable scaffolds can potentially be employed to bu...
82 CitationsSource
#1Dominic C. Chow (NU: Northwestern University)H-Index: 9
#2Larissa A. Wenning (NU: Northwestern University)H-Index: 3
Last. E. Terry Papoutsakis (NU: Northwestern University)H-Index: 19
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Hematopoietic cells of various lineages are organized in distinct cellular architectures in the bone marrow hematopoietic compartment (BMHC). The homogeneous Kroghian model, which deals only with a single cell type, may not be sufficient to accurately describe oxygen transfer in the BMHC. Thus, for cellular architectures of physiological significance, more complex biophysical-transport models were considered and compared against simulations using the homogeneous Kroghian model. The effects of th...
336 CitationsSource
#1Dominic C. Chow (NU: Northwestern University)H-Index: 9
#2Larissa A. Wenning (NU: Northwestern University)H-Index: 3
Last. E. Terry Papoutsakis (NU: Northwestern University)H-Index: 19
view all 4 authors...
Human bone marrow (BM) is a tissue of complex architectural organization, which includes granulopoietic loci, erythroblastic islets, and lymphocytic nodules. Oxygen tension (pO2) is an important determinant of hematopoietic stem and progenitor cell proliferation and differentiation. Thus, understanding the impact of the BM architectural organization on pO2 levels in extravascular hematopoietic tissue is an important biophysical problem. However, currently it is impossible to measure pO2 levels a...
276 CitationsSource