Pamela A. Silver
Wyss Institute for Biologically Inspired Engineering
GeneNuclear poreImportinGene expressionMolecular biologyChemistryCytoplasmSynthetic biologySaccharomyces cerevisiaeNuclear proteinRNA-binding proteinNuclear localization sequenceGeneticsNuclear export signalBiochemistryNuclear transportRNAComputational biologyBiologyCell biology
402Publications
120H-index
29.9kCitations
Publications 377
Newest
#1Monica P. McNerney (Wyss Institute for Biologically Inspired Engineering)H-Index: 1
#1Monica P. McNerney (Wyss Institute for Biologically Inspired Engineering)H-Index: 7
Last. Pamela A. Silver (Wyss Institute for Biologically Inspired Engineering)H-Index: 120
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Synthetic biology seeks to redesign biological systems to perform novel functions in a predictable manner. Recent advances in bacterial and mammalian cell engineering include the development of cells that function in biological samples or within the body as minimally invasive diagnostics or theranostics for the real-time regulation of complex diseased states. Ex vivo and in vivo cell-based biosensors and therapeutics have been developed to target a wide range of diseases including cancer, microb...
1 CitationsSource
#1Julianna Y. Lee (Harvard University)H-Index: 1
#2Andyna VernetH-Index: 8
Last. Pamela A. SilverH-Index: 120
view all 7 authors...
Erythropoietin enhances oxygen delivery and reduces hypoxia-induced cell death, but its pro-thrombotic activity is problematic for use of erythropoietin in treating hypoxia. We constructed a fusion protein that stimulates red blood cell production and neuroprotection without triggering platelet production, a marker for thrombosis. The protein consists of an anti-glycophorin A nanobody and an erythropoietin mutant (L108A). The mutation reduces activation of erythropoietin receptor homodimers that...
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#1Christine R. Zheng (Wyss Institute for Biologically Inspired Engineering)H-Index: 3
#1Christine R. Zheng (Wyss Institute for Biologically Inspired Engineering)
Last. Elizabeth A. Libby (Wyss Institute for Biologically Inspired Engineering)
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A lack of direct single-cell readouts for bacterial kinase activity remains a major barrier to our understanding of most signaling systems. At the single-cell-level, protein kinase activity is typically inferred by the activity of downstream transcriptional reporters. Complicating this approach in vivo, promoters are often co-regulated by several pathways, making the activity of a specific kinase difficult to deconvolve. Here, we have designed and constructed new, direct and specific sensors of ...
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#1David T. Riglar (Harvard University)H-Index: 19
#2David L. Richmond (Harvard University)H-Index: 14
Last. Pamela A. Silver (Wyss Institute for Biologically Inspired Engineering)H-Index: 120
view all 10 authors...
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#1Wiggert J. Altenburg (Harvard University)H-Index: 2
#2Nathan J Rollins (Wyss Institute for Biologically Inspired Engineering)H-Index: 4
Last. Tobias W. GiessenH-Index: 20
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Encapsulins are recently discovered protein compartments able to specifically encapsulate cargo proteins in vivo. Encapsulation is dependent on C-terminal targeting peptides (TPs). Here, we characterize and engineer TP-shell interactions in the Thermotoga maritima and Myxococcus xanthus encapsulin systems. Using force-field modeling and particle fluorescence measurements we show that TPs vary in native specificity and binding strength, and that TP-shell interactions are determined by hydrophobic...
5 CitationsSource
#1E. J. Olson (Harvard University)H-Index: 1
#2David M. BrownH-Index: 4
Last. Pamela A. SilverH-Index: 120
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Suppression of the host intracellular innate immune system is an essential aspect of viral replication. Here, we developed a suite of medium-throughput high-content cell-based assays to reveal the effect of individual coronavirus proteins on antiviral innate immune pathways. Using these assays, we screened the 196 protein products of seven coronaviruses (SARS-CoV-2, SARS-CoV-1, 229E, NL63, OC43, HKU1 and MERS). This includes a previously unidentified gene in SARS-CoV-2 encoded within the Spike g...
1 CitationsSource
#1Craig Westover (Cornell University)H-Index: 5
#2Najjar D (Cornell University)
Last. Christopher E. Mason (Cornell University)H-Index: 72
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Spaceflight has been documented to produce a number of detrimental effects to physiology and genomic stability, partly a result of Galactic Cosmic Radiation (GCR). In recent years, extensive research into extremotolerant organisms has begun to reveal how they survive harsh conditions, such as ionizing radiation. One such organism is the tardigrade (Ramazzottius varieornatus) which can survive up to 5kGy of ionizing radiation and also survive the vacuum of space. In addition to their extensive ne...
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#1Shannon N. Nangle (Wyss Institute for Biologically Inspired Engineering)H-Index: 6
#2Marika Ziesack (Wyss Institute for Biologically Inspired Engineering)H-Index: 8
Last. Pamela A. Silver (Wyss Institute for Biologically Inspired Engineering)H-Index: 120
view all 7 authors...
Abstract Coupling recent advancements in genetic engineering of diverse microbes and gas-driven fermentation provides a path towards sustainable commodity chemical production. Cupriavidus necator H16 is a suitable species for this task because it effectively utilizes H2 and CO2 and is genetically tractable. Here, we demonstrate the versatility of C. necator for chemical production by engineering it to produce three products from CO2 under lithotrophic conditions: sucrose, polyhydroxyalkanoates (...
6 CitationsSource
#1Bryan B. Hsu (VT: Virginia Tech)H-Index: 15
#2Isaac N. Plant (Harvard University)H-Index: 2
Last. Pamela A. Silver (Harvard University)H-Index: 120
view all 6 authors...
Abundant links between the gut microbiota and human health indicate that modification of bacterial function could be a powerful therapeutic strategy. The inaccessibility of the gut and inter-connections between gut bacteria and the host make it difficult to precisely target bacterial functions without disrupting the microbiota and/or host physiology. Herein we describe a multidisciplinary approach to modulate the expression of a specific bacterial gene within the gut by oral administration. We d...
6 CitationsSource
#1James Kuo (Wyss Institute for Biologically Inspired Engineering)H-Index: 9
#2Ruoshi Yuan (Harvard University)H-Index: 14
Last. Pamela A. Silver (Wyss Institute for Biologically Inspired Engineering)H-Index: 120
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In synthetic circuits, CRISPR-Cas systems have been used effectively for endpoint changes from an initial state to a final state, such as in logic gates. Here, we use deactivated Cas9 (dCas9) and deactivated Cas12a (dCas12a) to construct dynamic RNA ring oscillators that cycle continuously between states over time in bacterial cells. While our dCas9 circuits using 103-nt guide RNAs showed irregular fluctuations with a wide distribution of peak-to-peak period lengths averaging approximately nine ...
2 CitationsSource