De-Quan Li
Baylor College of Medicine
Matrix metalloproteinasePathologyMolecular biologyProinflammatory cytokineChemistryProgenitor cellImmunologyConjunctivaCorneaInflammationCytokineCorneal epitheliumAllergic inflammationWound healingFibroblastStem cellPopulationDry Eye SyndromesEpitheliumSignal transductionMedicineBiologyCell biology
176Publications
64H-index
10.1kCitations
Publications 181
Newest
#1Jinmiao Li (BCM: Baylor College of Medicine)H-Index: 2
#2Rong Lu (SYSU: Sun Yat-sen University)H-Index: 6
Last. De-Quan Li (BCM: Baylor College of Medicine)H-Index: 64
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PURPOSE To explore the distinct expression and diverse roles of IL-36 cytokines in dry eye disease using an in vitro hyperosmolarity model of human corneal epithelial cells (HCECs). METHODS Primary HCECs were cultured from fresh donor limbal explants. Hyperosmolarity model was established by switching HCECs from isosmotic (312 mOsM) to hyperosmotic medium (350-500 mOsM) alone or with addition of rhIL-36RA or rhIL-38 for 2-48 h. Some cultures were treated with IL-36α (1-10 ng/ml) with or without ...
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#1Jinmiao Li (SYSU: Sun Yat-sen University)H-Index: 2
#2Sangbae Kim (BCM: Baylor College of Medicine)H-Index: 4
Last. De-Quan Li (BCM: Baylor College of Medicine)H-Index: 64
view all 9 authors...
Purpose null Differentiated from adult stem cells (ASCs), transit-amplifying cells (TACs) play an important role in tissue homeostasis, development, and regeneration. This study aimed to characterize the gene expression profile of a candidate TAC population in limbal basal epithelial cells using single-cell RNA sequencing (scRNA-seq). null Methods null Single cells isolated from the basal corneal limbus were subjected to scRNA-seq using the 10x Genomics platform. Cell types were clustered by gra...
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#1Jinmiao Li (SYSU: Sun Yat-sen University)H-Index: 2
#2Yun Zhang (WMU: Wenzhou Medical College)H-Index: 2
Last. De-Quan Li (BCM: Baylor College of Medicine)H-Index: 64
view all 4 authors...
#1De-Quan Li (BCM: Baylor College of Medicine)H-Index: 64
#2Jinmiao Li (SYSU: Sun Yat-sen University)H-Index: 2
Last. Rui Chen (BCM: Baylor College of Medicine)H-Index: 89
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#9Jin Li (BCM: Baylor College of Medicine)
Abstract Purpose This study aimed to uncover novel cell types in heterogenous basal limbus of human cornea for identifying LSC at single cell resolution. Methods Single cells of human limbal basal epithelium were isolated from young donor corneas. Single-cell RNA-Sequencing was performed using 10x Genomics platform, followed by clustering cell types through the graph-based visualization method UMAP and unbiased computational informatic analysis. Tissue RNA in situ hybridization with RNAscope, im...
4 CitationsSource
#1Zhao Liu (BCM: Baylor College of Medicine)H-Index: 1
#2Ding Chen (BCM: Baylor College of Medicine)H-Index: 2
Last. De-Quan Li (BCM: Baylor College of Medicine)H-Index: 64
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Inflammation is the main pathophysiology of dry eye, characterized by tear film instability and hyperosmolarity. The aim of this study was to investigate the association of inflammation and cellular autophagy using an in vitro dry eye model with primary cultured human corneal epithelial cells (HCECs). Primary HCECs cultured with fresh limbal explants from donors were switched to a hyperosmotic medium (450 mOsM) by adding sodium chloride into the culture medium. We observed the stimulated inflamm...
3 CitationsSource
#1Zhao Liu (Xi'an Jiaotong University)H-Index: 1
#2Ding Chen (WMU: Wenzhou Medical College)H-Index: 2
Last. De-Quan Li (BCM: Baylor College of Medicine)H-Index: 64
view all 10 authors...
Purpose Autophagy plays an important role in balancing the inflammatory response to restore homeostasis. The aim of this study was to explore the mechanism by which trehalose suppresses inflammatory cytokines via autophagy activation in primary human corneal epithelial cells (HCECs) exposed to hyperosmotic stress. Methods An in vitro dry eye model was used in which HCECs were cultured in hyperosmolar medium with the addition of sodium chloride (NaCl). Trehalose was applied in different concentra...
8 CitationsSource
#1De-Quan Li (BCM: Baylor College of Medicine)H-Index: 64
#2Sangbae Kim (BCM: Baylor College of Medicine)H-Index: 4
Last. Rui Chen (BCM: Baylor College of Medicine)H-Index: 89
view all 8 authors...
#1Jiaoyue Hu (BCM: Baylor College of Medicine)H-Index: 2
#2Ning Gao (Xi'an Jiaotong University)H-Index: 2
Last. De-Quan Li (BCM: Baylor College of Medicine)H-Index: 64
view all 11 authors...
This study was to explore a novel IL-33/ST2/IL-9/IL-9R signaling pathway that disrupts ocular surface barrier and amplifies allergic inflammation. Two murine models of experimental allergic conjunctivitis (EAC) and IL-9 topical challenge in wild type Balb/c and ST2−/− mice, and two culture models of primarily human corneal epithelial cells (HCECs) and mouse CD4+ T cells were performed. Clinical manifestations, Oregon-Green Dextran (OGD) staining, the apical junction complexes (AJCs), IL-33/ST2 a...
1 CitationsSource
#1Ruzhi Deng (WMU: Wenzhou Medical College)H-Index: 11
#2Xin Chen (WMU: Wenzhou Medical College)H-Index: 5
Last. De-Quan Li (BCM: Baylor College of Medicine)H-Index: 64
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This study was to explore the role and mechanism of macrophages in pollen-triggered allergic inflammation. A murine model of short ragweed (SRW) pollen-induced experimental allergic conjunctivitis (EAC), and bone marrow (BM)-macrophages cultures were used. Typical allergic manifestations and TSLP-stimulated Th2 hyperresponse were observed in ocular surface of EAC model in wild-type (WT) mice induced by SRW. The M2 phenotype markers, Arg1, Ym1 and FIZZ1, were highly expressed by conjunctiva and d...
6 CitationsSource