Marco Antonio González-López
Universidad Autónoma de la Ciudad de México
GeneSiderophoreGlyceraldehyde 3-phosphate dehydrogenaseGenomeMembrane proteinBacterial outer membraneHSP60Peptide sequenceMetalloproteinBacterial growthChemistryCytoplasmHuman pathogenIn silicoHemeDehydrogenaseHelicobacter pyloriPathogenGastritisHemoglobinLactoferrinStreptococcus pneumoniaeHemoglobin bindingRecombinant DNAPlasma protein bindingBacteriaEscherichia coliBiochemistryCell growthChaperoninBiologyGroELMicrobiology
5Publications
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Publications 5
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Introduction: Helicobacter pylori is a gram-negative spiral bacterial, has been associated with peptic ulcers, gastritis, duodenitis, and is believed to be the causative agent of gastric cancer. The sources such as human lactoferrin, haem and haemoglobin can support the H. pylori growth. However, still not fully understood how the process of iron acquisition occurs. An in silico analysis has shown that H. pylori genome has a family of three outer membrane protein regulated by iron (FrpB). Two of...
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#1Zelene Edith Vázquez-Zamorano (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 1
#2Marco Antonio González-López (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 4
Last. José de Jesús Olivares-Trejo (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 13
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Streptococcus pneumoniae is a gram positive encapsulated bacterium responsible of septicaemia and upper respiratory infections in children. This pathogen requires iron to survive in the host, which it can obtain of haemoglobin (Hb) or haem. Only two Hb-binding membrane proteins have been identified up to now. However it is unknown whether this pathogen secretes proteins in order to scavenge iron from the Hb or haem. Therefore, in order to explore these possibilities, cellular growth of S. pneumo...
6 CitationsSource
#1Marco Antonio González-López (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 4
Last. José de Jesús Olivares-Trejo (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 13
view all 4 authors...
Helicobacter pylori is a bacterium that can use multiple iron sources. However, it is unknown whether this bacterium secretes molecules such as siderophores or haemophores to scavenge iron. Here, we report the first secreted iron-binding protein of H. pylori, which we purified by haem-affinity chromatography. Mass spectrometry analysis revealed its identity as chaperonin (HpGroEL). When we compared HpGroEL with EcGroEL from Escherichia coli, they were homologous, showing 60% similarity. Addition...
24 CitationsSource
#1María Elena Romero-Espejel (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 4
#2Marco Antonio González-López (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 4
Last. José de Jesús Olivares-Trejo (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 13
view all 3 authors...
Streptococcus pneumoniae, a human pathogen bacterium, can support its growth using haemoglobin (Hb) and haem as sole iron sources, but not when holo-transferrin or holo-lactoferrin is supplied. For this reason, it is easy to think that the principal iron sources for this pathogen inside humans are Hb and haem. Unfortunately, the mechanism has been poorly studied. The findings presented in this study are the first efforts that attempted to explain the mechanism involved in iron acquisition of thi...
24 CitationsSource
#1Marco Antonio González-López (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 4
#2José de Jesús Olivares-Trejo (UACM: Universidad Autónoma de la Ciudad de México)H-Index: 13
Human hemoglobin (Hb) is a metalloprotein used by pathogens as a source of iron during invasive process. It can support the Helicobacter pylori growth and several proteins are induced during iron starvation. However, the identity of those proteins remains unknown. In this work, by in silico analysis we identified FrpB2 in H. pylori genome. This protein was annotated as an iron-regulated outer membrane protein. Multiple amino acid alignment showed the motifs necessary for Hb-binding. We demonstra...
16 CitationsSource