Erik Sahai
Francis Crick Institute
CancerCytoskeletonPathologyCellStromal cellKinaseChemistryExtracellular matrixImmunologyTumor microenvironmentRHOAMelanomaMetastasisCancer researchMotilitySignal transductionMedicineCell cultureCell adhesionBiologyCell biologyCancer cell
162Publications
71H-index
21.8kCitations
Publications 150
Newest
#1Anna M. Dowbaj (Francis Crick Institute)H-Index: 2
#2Robert P. Jenkins (Francis Crick Institute)H-Index: 8
Last. Erik Sahai (Francis Crick Institute)H-Index: 71
view all 11 authors...
The shuttling of transcription factors and transcriptional regulators into and out of the nucleus is central to the regulation of many biological processes. Here we describe a new method for studying the rates of nuclear entry and exit of transcriptional regulators. A photo-responsive AsLOV (Avena sativa Light Oxygen Voltage) domain is used to sequester fluorescently-labelled transcriptional regulators YAP1 and TAZ/WWTR1 on the surface of mitochondria and reversibly release them upon blue light ...
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#1Yue ZhaoH-Index: 1
#2Xiao Fu (Francis Crick Institute)H-Index: 4
Last. Kevin Litchfield (UCL: University College London)H-Index: 8
view all 31 authors...
The genetic evolutionary features of solid tumour growth are becoming increasingly well described, but the spatial and physical nature of subclonal growth remains unclear. Here, we utilize 102 macroscopic whole-tumour images from clear cell renal cell carcinoma patients, with matched genetic and phenotypic data from 756 biopsies. Utilizing a digital image processing pipeline, a renal pathologist marked the boundaries between tumour and normal tissue and extracted positions of boundary line and b...
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#1Manuela Zangrossi (UNIPD: University of Padua)H-Index: 2
#2Patrizia RomaniH-Index: 5
Last. Marco MontagnerH-Index: 14
view all 11 authors...
Late relapse of disseminated cancer cells is a common feature of breast and prostate tumors. Several intrinsic and extrinsic factors have been shown to affect quiescence and reawakening of disseminated dormant cancer cells (DDCCs); however, the signals and processes sustaining the survival of DDCCs in a foreign environment are still poorly understood. We have recently shown that crosstalk with lung epithelial cells promotes survival of DDCCs of estrogen receptor-positive (ER+) breast tumors. By ...
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#1Manuela Zangrossi (UNIPD: University of Padua)H-Index: 2
#2Probir Chakravarty (Francis Crick Institute)H-Index: 33
Last. Marco Montagner (UNIPD: University of Padua)H-Index: 14
view all 7 authors...
(1) Background: metastatic relapse following a prolonged period of disease-free survival is a common cause of mortality for many cancer patients. Disseminated dormant cancer cells (DDCCs) lie below the radar before waking up years, or even decades, after the removal of the primary tumor. This implies that they are able to survive in a latent state in a foreign environment for an extended period of time supported by intrinsic and extrinsic factors still to be elucidated. (2) Methods: we employed ...
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#1Xiao FuH-Index: 20
#2Yue ZhaoH-Index: 1
Last. Scott ShepherdH-Index: 3
view all 33 authors...
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#1Hiroshi Kondo (Francis Crick Institute)H-Index: 2
#2Colin D.H. Ratcliffe (Francis Crick Institute)H-Index: 2
Last. Erik Sahai (Francis Crick Institute)H-Index: 71
view all 9 authors...
Summary Inter-cellular heterogeneity in metabolic state has been proposed to influence many cancer phenotypes, including responses to targeted therapy. Here, we track the transitions and heritability of metabolic states in single PIK3CA mutant breast cancer cells, identify non-genetic glycolytic heterogeneity, and build on observations derived from methods reliant on bulk analyses. Using fluorescent biosensors in vitro and in tumors, we have identified distinct subpopulations of cells whose glyc...
6 CitationsSource
#1Esther Wershof (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 3
#2Danielle Park (Francis Crick Institute)H-Index: 10
Last. Paul A. Bates (Francis Crick Institute)H-Index: 67
view all 11 authors...
Diverse extracellular matrix patterns are observed in both normal and pathological tissue. However, most current tools for quantitative analysis focus on a single aspect of matrix patterning. Thus, an automated pipeline that simultaneously quantifies a broad range of metrics and enables a comprehensive description of varied matrix patterns is needed. To this end, we have developed an ImageJ plugin called TWOMBLI, which stands for The Workflow Of Matrix BioLogy Informatics. This pipeline includes...
1 CitationsSource
#2Takuya Kato (Francis Crick Institute)H-Index: 3
Last. Anna LabernadieH-Index: 8
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In many solid tumors, collective cell invasion prevails over single-cell dissemination strategies. Collective modes of invasion often display specific front/rear cellular organization, where invasive leader cells arise from cancer cell populations or the tumor stroma. Collective invasion involves coordinated cellular movements which require tight mechanical crosstalk through specific combinations of cell-cell interactions and cell-matrix adhesions. Cancer Associated Fibroblasts (CAFs) have been ...
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#1Oshrat Levi-Galibov (Weizmann Institute of Science)H-Index: 2
#2Hagar Lavon (Weizmann Institute of Science)H-Index: 2
Last. Ruth Scherz-Shouval (Weizmann Institute of Science)H-Index: 17
view all 20 authors...
In the colon, long-term exposure to chronic inflammation drives colitis-associated colon cancer (CAC) in patients with inflammatory bowel disease. While the causal and clinical links are well established, molecular understanding of how chronic inflammation leads to the development of colon cancer is lacking. Here we deconstruct the evolving microenvironment of CAC by measuring proteomic changes and extracellular matrix (ECM) organization over time in a mouse model of CAC. We detect early changes...
4 CitationsSource
#1Manuela Zangrossi (UNIPD: University of Padua)H-Index: 2
#2Probir Chakravarty (Francis Crick Institute)H-Index: 33
Last. Marco Montagner (UNIPD: University of Padua)H-Index: 14
view all 11 authors...
Late relapse of disseminated cancer cells is a common feature of some types of tumors. Several intrinsic and extrinsic factors have been shown to affect reawakening of disseminated dormant cancer cells (DDCCs); however, the signals and processes sustaining survival of DDCCs in a foreign environment are still poorly understood. We have recently shown that crosstalk with lung epithelial cells promotes persistence of DDCCs from estrogen receptor positive (ER+) breast tumors. Here we show that TFEB-...
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