Shubhada M. Naik
Emory University
PromoterMessenger RNAIRF8PhosphorylationEndothelial stem cellInternal medicineDownregulation and upregulationCC chemokine receptorsResponse elementChemokineEndocrinologyCellGene expressionMolecular biologyJAK-STAT signaling pathwayVirologyNeurotrophic factorsCell adhesion moleculeRegulation of gene expressionReceptorSTAT proteinNorthern blotTumor necrosis factor alphaCalcium in biologyChemistryIn vitroImmunologyGrowth factorT cellTyrosineInterferonSubstance PCorneaVCAM-1EndotheliumSTAT1Cutaneous nerveNeurokinin AKeratinocyteHuman skinTyrosine phosphorylationNerve growth factorCytokineExtravasationParallel-plate flow chamberPhorbolInterferon gammaNF-kappa B p50 SubunitIntercellular Adhesion Molecule-1Nerve growth factor productionNFATC Transcription FactorsIRF1Expression vectorPertussis toxinTransfectionSignal transductionInterleukin 18Palindromic sequenceIntracellularCell cultureBlotProtein tyrosine phosphataseTranscription factorBiologyInterferon regulatory factorsC-C chemokine receptor type 6DNA-binding proteinCell biology
10Publications
10H-index
497Citations
Publications 10
Newest
#1Amy K. Gira (Emory University)H-Index: 6
#2Katherine A. Casper (Emory University)H-Index: 7
Last. Robert A. Swerlick (Emory University)H-Index: 44
view all 6 authors...
Interferon regulatory factor-1 is a transcription factor that is linked to the expression of genes important in the initiation of the inflammatory response and the control of cell cycle. In this study, we determined that the generation of interferon regulatory factor-1 expression in human dermal microvascular endothelial cells was transcriptionally mediated by tumor necrosis factor-α or interferon-γ via iron-dependent pathways. The induction of interferon regulatory factor-1 protein and the upre...
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#1Guido J. Burbach (Emory University)H-Index: 3
#2Kyu Han Kim (SNU: Seoul National University)H-Index: 43
Last. Cheryl A. Armstrong (Emory University)H-Index: 32
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Nerve growth factor is an essential neurotrophic factor required for the growth and maintenance of cutaneous sensory nerves. In the skin, keratinocytes are a significant source of nerve growth factor; however, the regulation of cutaneous nerve growth factor production still remains to be fully understood. In this study we tested the hypothesis that neuropeptides released by cutaneous sensory nerves have the capacity to modulate directly the expression of keratinocyte nerve growth factor, which w...
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#1Guido J. Burbach (Emory University)H-Index: 3
#2Shubhada M. NaikH-Index: 10
Last. John C. AnselH-Index: 53
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Purpose. The interferon-?-inducing factor Interleukin-18 (IL-18) is a recently described cytokine that appears to have multiple important pro-inflammatory effects including the induction of interferon-? (IFN-?) by activated T-cells. The expression of IL-18 by human cornea has not been previously reported. In the present study, we examine the possibility that human corneal epithelial cells are capable of producing this leukocyte-activating factor which may play an important role in IFN-?-dependen...
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#1David J. Fitzhugh (Emory University)H-Index: 5
#2Shubhada M. Naik (Emory University)H-Index: 10
Last. Samuel T Hwang (Emory University)H-Index: 50
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We asked whether CCR6, which is found on 50% of memory, but not naive, T cells is associated with the ability of memory T cells (mTC) to arrest on TNF-? activated endothelial cells under flow conditions. CCR6-, CCR6+, and total mTC were fractionated by magnetic-bead selection using CCR6 and CD45RA mAbs. TNF-? stimulation of human dermal microvascular endothelial cells (HDMEC) induced the mRNA for LARC/MIP-3? (the chemokine ligand for CCR6) by 200-fold as measured by real-time, quantitative RT-PC...
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#1Kimberly L. Quinlan (Emory University)H-Index: 6
#2Shubhada M. Naik (Emory University)H-Index: 10
Last. S W Caughman (Emory University)H-Index: 7
view all 7 authors...
Upon stimulation, cutaneous sensory nerves release neuropeptides such as substance P (SP), which modulate responses in the skin by activating a number of target cells via neurokinin receptors. We have demonstrated that SP preferentially binds to the NK-1R on human dermal microvascular cells, resulting in increased intracellular Ca2+ and induction of ICAM-1 and VCAM-1 expression. In the current studies, we identify specific elements in the regulatory regions of ICAM-1 and VCAM-1 genes as necessar...
#1Shubhada M. Naik (Emory University)H-Index: 10
#2Georgetta Cannon (Emory University)H-Index: 2
Last. Josiah N. Wilcox (Emory University)H-Index: 67
view all 8 authors...
Interleukin-18 is a potent inducer of interferon-γ by activated T cells, macrophages, and monocytes and is synthesized as an inactive precursor. Pro-interleukin-18 must be cleaved by interleukin-1-β-converting enzyme for secretion of the biologically active form. We report that among selected non-bone marrow derived skin cells, interleukin-18 mRNA is constitutively expressed by human keratinocytes and not by dermal microvascular endothelial cells, dermal fibroblasts, or melanocytes. Interleukin-...
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#1Kimberly L. Quinlan (Emory University)H-Index: 6
#2In Sung Song (Emory University)H-Index: 5
Last. John C. Ansel (Emory University)H-Index: 53
view all 9 authors...
Sensory nerves in skin are capable of releasing multiple neuropeptides, which modulate inflammatory responses by activating specific cutaneous target cells. Extravasation of particular subsets of leukocytes depends upon the regulated expression of cellular adhesion molecules such as VCAM-1 on microvascular endothelial cells. We examined the direct effect of cutaneous neuropeptides on the expression and function of human dermal microvascular endothelial cell (HDMEC) VCAM-1. A significant increase...
#1Sherry M. OwenH-Index: 18
#2Donna L. RudolphH-Index: 25
Last. Renu B. LalH-Index: 59
view all 7 authors...
In vitro infection of T cells with human T lymphotropic virus types I and II (HTLV-I and HTLV-II) resulted in constitutive expression of ICAM-1. Higher levels of ICAM-1 mRNA were expressed in HTLV-transformed cell lines (MT-2, MoT, C8166) when compared with uninfected T cell lines (A301). We demonstrate that this activation is conferred through a site on the ICAM-1 promoter that is activated in trans by the Tax protein of HTLV-I and HTLV-II. Enhanced promoter activity was detected when the ICAM-...
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#1Jennifer L. Duff (Emory University)H-Index: 10
#2Kimberly L. Quinlan (Emory University)H-Index: 6
Last. S. Wright Caughman (Emory University)H-Index: 19
view all 5 authors...
Differential expression of intercellular adhesion molecule-1 (ICAM-1) in the epidermis plays a critical role in the regulation of cutaneous inflammation, immunologic reactions, and tissue repair. Transcriptional upregulation of ICAM-1 in response to interferon- γ (IFN γ ) occurs through a palindromic response element pI γ RE, pI γ RE is homologous to IFN γ -activated sequences, which bind to tyrosine phosphorylated members of the transcription factor family known as signal transducers and activa...
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#1Shubhada M. Naik (Emory University)H-Index: 10
#2Naotaka Shibagaki (Emory University)H-Index: 3
Last. S. Wright Caughman (Emory University)H-Index: 19
view all 6 authors...
Abstract In response to interferon γ (IFNγ), intercellular adhesion molecule-1 (ICAM-1) is expressed on human keratinocytes, a cell type that is critically involved in cutaneous inflammation. An ICAM-1 5′ regulatory region palindromic response element, pIγRE, has been shown to confer IFNγ-dependent transcription enhancement. By electrophoretic mobility shift assays (EMSA), pIγRE forms a distinct complex with proteins from IFNγ-treated human keratinocytes, termed γ response factor (GRF). Binding ...
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