Mian Wan
Sichuan University
GeneAmeloblastRegenerative medicineDownregulation and upregulationPathologymicroRNACellular differentiationEnamel paintRegeneration (biology)ChemistryDNA methylationMesenchymal stem cellProgenitor cellDental pulp stem cellsStem cellEpigeneticsGeneticsHomeostasisHistoneMedicineBiologyCell biology
Publications 24
#1Dixin Cui (Sichuan University)H-Index: 5
#2Sihan Yu (Sichuan University)
Last. Mian Wan (Sichuan University)H-Index: 10
view all 8 authors...
Dental caries and trauma always lead to pulp necrosis and subsequent root development arrest of young permanent teeth. The traditional treatment, apexification, with absence of further root formation, results in abnormal root morphology and compromises long-term prognosis. Regeneration endodontics procedures (REPs) have been developed and considered as an alternative strategy for management of immature permanent teeth with pulpal necrosis, including cell-free REPs and cell-based REPs. Cell-free ...
#1Hui Li (Sichuan University)H-Index: 7
#1Hongyu Li (Sichuan University)H-Index: 3
Last. Mian Wan (Sichuan University)H-Index: 10
view all 9 authors...
Enamel formation is a serial and complex biological process, during which related genes are expressed progressively in a spatiotemporal manner. This process is vulnerable to environmental cues, resulting in developmental defects of enamel (DDE). However, how environmental factors are biologically integrated during enamel formation is still poorly understood. Here, we investigated the mechanism of DDE elicited by a model endocrine-disrupting chemical, bisphenol A (BPA), in mouse incisors. We show...
#2Ying LiuH-Index: 3
Last. Mian WanH-Index: 10
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Tooth enamel, a highly mineralized tissue covering the outermost area of teeth, is always damaged by dental caries or trauma. Tooth enamel rarely repairs or renews itself, due to the loss of ameloblasts and dental epithelial stem cells (DESCs) once the tooth erupts. Unlike human teeth, mouse incisors grow continuously due to the presence of DESCs that generate enamel-producing ameloblasts and other supporting dental epithelial lineages. The ready accessibility of mouse DESCs and wide availabilit...
#1Dan Zhou (Sichuan University)
#2Lu Gan (Sichuan University)H-Index: 1
Last. Wei Du (Sichuan University)H-Index: 8
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Epigenetic regulation, mainly involving DNA methylation, histone modification, and noncoding RNAs, affects gene expression without modifying the primary DNA sequence and modulates cell fate. Mesenchymal stem cells derived from dental pulp, also called dental pulp stem cells (DPSCs), exhibit multipotent differentiation capacity and can promote various biological processes, including odontogenesis, osteogenesis, angiogenesis, myogenesis, and chondrogenesis. Over the past decades, increased attenti...
#1Lu Gan (Sichuan University)H-Index: 1
#2Ying Liu (Sichuan University)H-Index: 3
Last. Mian Wan (Sichuan University)H-Index: 10
view all 6 authors...
Human mesenchymal stem cells (hMSCs) are multipotent cells, which exhibit plastic adherence, express specific cell surface marker spectrum, and have multi-lineage differentiation potential. These cells can be obtained from multiple tissues. Dental tissue-derived hMSCs (dental MSCs) possess the ability to give rise to mesodermal lineage (osteocytes, adipocytes, and chondrocytes), ectodermal lineage (neurocytes), and endodermal lineages (hepatocytes). Dental MSCs were first isolated from dental pu...
6 CitationsSource
#1Si Wu (Sichuan University)H-Index: 3
#2Yachuan Zhou (Sichuan University)H-Index: 9
Last. Liwei Zheng (Sichuan University)H-Index: 17
view all 10 authors...
The pulpotomy with pulp capping is aimed at retaining vital pulp with reparative dentin formation. Vascular endothelial growth factor (VEGF) plays a crucial role in dentin regeneration; however, its constant administrations in the human body is still problematic. Chitosan was widely studied as an effective carrier to deliver bioactive molecules in regenerative medicine. In this study, we conducted a chitosan/β-glycerophosphate (CS/β-GP) hydrogel as a VEGF-sustained release system and explored it...
8 CitationsSource
#1Dixin Cui (Sichuan University)H-Index: 5
#2Jiani Xiao (Sichuan University)H-Index: 1
Last. Liwei Zheng (Sichuan University)H-Index: 17
view all 12 authors...
OBJECTIVES: The odontoblastic differentiation of dental pulp stem cells (DPSCs) contributes to tertiary dentin formation. Our previous study indicated that epiregulin (EREG) enhanced odontogenesis potential of dental pulp. Here, we explored the effects of EREG during DPSC odontoblastic differentiation. METHODS: The changes in EREG were detected during tertiary dentin formation. DPSCs were treated with recombinant human EREG (rhEREG), EREG receptor inhibitor gefitinib and short hairpin RNAs. The ...
9 CitationsSource
#1Tao Zhou (Sichuan University)H-Index: 3
#2Jinhai Pan (Sichuan University)H-Index: 1
Last. Xin Zhou (Sichuan University)H-Index: 6
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Dental follicle cells (DFCs) are a group of mesenchymal progenitor cells surrounding the tooth germ, responsible for cementum, periodontal ligament, and alveolar bone formation in tooth development. Cascades of signaling pathways and transcriptional factors in DFCs are involved in directing tooth eruption and tooth root morphogenesis. Substantial researches have been made to decipher multiple aspects of DFCs, including multilineage differentiation, senescence, and immunomodulatory ability. DFCs ...
20 CitationsSource
#1Amnon Sharir (UCSF: University of California, San Francisco)H-Index: 18
#2Pauline Marangoni (UCSF: University of California, San Francisco)H-Index: 13
Last. Ophir D. Klein (UCSF: University of California, San Francisco)H-Index: 54
view all 15 authors...
The classical model of tissue renewal posits that small numbers of quiescent stem cells (SCs) give rise to proliferating transit-amplifying cells before terminal differentiation. However, many organs house pools of SCs with proliferative and differentiation potentials that diverge from this template. Resolving SC identity and organization is therefore central to understanding tissue renewal. Here, using a combination of single-cell RNA sequencing (scRNA-seq), mouse genetics and tissue injury app...
21 CitationsSource
#1Ruoshi Xu (Sichuan University)H-Index: 6
#2Daimo Guo (Sichuan University)H-Index: 1
Last. Liwei Zheng (Sichuan University)H-Index: 17
view all 10 authors...
Bone remodelling keeps going through the lifespan of human by bone formation and bone resorption. In the craniofacial region, mandibles act as the main force for biting and chewing, and also become susceptible to a common bone-loss disease, namely, apical periodontitis, once infected dental pulp is not treated timely, during which bone resorption occurs from the apical foramen to the apical bone area. Although conventional root canal treatment (RCT) can remove the most of the infection, chronica...
4 CitationsSource