Jianhui Shi
Geisinger Medical Center
CancerH&E stainPathologyCellCytologyTissue microarrayImmunohistochemistryDysplasiaSquamous intraepithelial lesionBiopsyFine-needle aspirationGallbladderRenal cell carcinomaCarcinomaTumor suppressor geneClear cellPancreasMaspinAdenocarcinomaClear cell carcinomaAtypiaPancreatic Intraepithelial NeoplasiaMetastatic breastImmunostainingAnatomical pathologyMedicineStainingIn situ hybridizationBiology
20Publications
13H-index
825Citations
Publications 20
Newest
#1Iman Sarami (Geisinger Medical Center)
#2Jianhui Shi (Geisinger Medical Center)H-Index: 13
Last. Fan Lin (Geisinger Medical Center)H-Index: 25
view all 6 authors...
OBJECTIVES Human kidney injury molecule 1 (hKIM-1) is a sensitive and specific marker for detection of clear cell renal cell carcinoma (CRCC), papillary renal cell carcinoma (PRCC), and ovarian clear cell carcinoma (OCCC). Its use was limited to a few surgical pathology laboratories because this specific antibody to hKIM-1 was not commercially available. We investigated the diagnostic utility of RNA in situ hybridization/RNAscope in the detection of hKIM-1 in tumors from various organs. METHODS ...
Source
#1Fan Lin (Geisinger Medical Center)H-Index: 25
#2Jianhui Shi (Geisinger Medical Center)H-Index: 13
Last. Haiyan Liu (Geisinger Medical Center)H-Index: 15
view all 8 authors...
Objectives: Inconsistent data on detection of albumin expression by ribonucleic acid (RNA) in situ hybridization have been reported. We investigated the utility of RNAscope (Advanced Cell Diagnostics, Hayward, CA) in detection of albumin in hepatocellular carcinomas (HCCs), intrahepatic cholangiocarcinomas (ICCs), and carcinomas from various organs using manual and automated staining. Methods: RNAscope for albumin detection was performed on 482 cases on tissue microarray sections and on 22 cases...
12 CitationsSource
#1Jianhui ShiH-Index: 13
#2Haiyan LiuH-Index: 15
Last. Fan LinH-Index: 25
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Context.—TTF-1 and napsin A immunomarkers have a crucial role in differentiating lung adenocarcinoma from lung squamous cell carcinoma and in identifying a primary lung adenocarcinoma when working on a tumor of unknown origin. Objectives.—To investigate the diagnostic sensitivity of ribonucleic acid in situ hybridization (RNAscope) in the detection of expression of these biomarkers in lung adenocarcinomas and to compare RNAscope to immunohistochemical techniques. Design.—Both RNAscope and the im...
6 CitationsSource
#1Fan Lin (Geisinger Medical Center)H-Index: 25
#2Jianhui Shi (Geisinger Medical Center)H-Index: 13
Standardization of every step in pre-analytic, analytic and post-analytic phases is crucial to achieve reproducible and reliable immunohistochemistry test results. This chapter delineates some critical points in pre-analytic, analytic and post-analytic phases, reiterates some important questions which may or may not have a consensus at this time, and updates the newly proposed guidelines on antibody validation from the College of American Pathologists Pathology and Laboratory Quality Center. Add...
4 CitationsSource
#1Fan Lin (Geisinger Medical Center)H-Index: 25
#2Jeffrey W. Prichard (Geisinger Medical Center)H-Index: 15
Last. Zongming E. Chen (Geisinger Medical Center)H-Index: 4
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The lack of detailed information about antibodies and staining protocols in the literature is quite frustrating, especially when trying to reproduce published results; therefore we have included detailed antibody and staining protocol information, including many recently described antibodies such as GATA3, PAX8, napsin A, p40, ERG, NKX3.1, NKX2.2, SATB2, cadherin 17, arginase-1, pVHL, maspin, UP II, SALL4, SF-1, TROP2, adipophilin, SOX10, Merkel cell polyomavirus, NY-ESO-1, BRAF, and c-MYC, in t...
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#1Haiyan Liu (Geisinger Medical Center)H-Index: 15
#2Jianhui Shi (Geisinger Medical Center)H-Index: 13
Last. Fan Lin (Geisinger Medical Center)H-Index: 25
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Objectives Estrogen receptor (ER), gross cystic disease fluid protein 15 (GCDFP-15), and mammaglobin (MGB) are commonly used breast-specific immunomarkers; however, about half of metastatic breast carcinomas are negative for all three. GATA-binding protein 3 (GATA-3) has emerged recently as a sensitive and relatively specific immunomarker for breast and urothelial carcinomas, but the data documenting its expression in ER-negative breast carcinomas are limited; this often poses a dilemma in the s...
38 CitationsSource
#1Fan Lin (Geisinger Medical Center)H-Index: 25
#2Jianhui ShiH-Index: 13
Last. Haiyan LiuH-Index: 15
view all 8 authors...
PMS2 in more than 80% of medullary carcinomas; (2) expression of cadherin-17 and SATB2 in 89% of medullary carcinomas; (3) focal expression of TFF3, MUC4, calretinin, CDX2, CK20, and synaptophysin in 72%, 72%, 67%, 67%, 28%, and 17% of 18 medullary carcinoma cases, respectively; and (4) expression of SATB2 and cadherin-17 in 97% and 98% of the colorectal adenocarcinomas, respectively, whereas their expression was seen in 3.6% and 3.3% of nongastrointestinal tumors, respectively. Conclusions.—We ...
64 CitationsSource
#1Haiyan Liu (Geisinger Medical Center)H-Index: 15
#2Jianhui Shi (Geisinger Medical Center)H-Index: 13
Last. Fan Lin (Geisinger Medical Center)H-Index: 25
view all 5 authors...
: The transmembrane protease, serine 2-E twenty-six related gene (TMPRSS2-ERG) fusion leading to ERG overexpression, was detected in approximately 50% of prostate cancers (ranging from 35-70%). However, the published data on ERG expression in tumors from other organs and normal tissues were limited. In this study, we investigated the expression of ERG in TMA sections of various normal tissues (N=452) and carcinomas (N=1,129) from various organs, including 90 cases of low to intermediate-grade (L...
22 Citations
#1Jianhui Shi (Geisinger Medical Center)H-Index: 13
#2Haiyan Liu (Geisinger Medical Center)H-Index: 15
Last. Fan Lin (Geisinger Medical Center)H-Index: 25
view all 5 authors...
Summary Our recent study demonstrated the up-regulation of maspin, IMP3, and S100P and down-regulation of von Hippel-Lindau gene product (pVHL) in ductal adenocarcinoma of the pancreas. Distinction of adenocarcinoma of the gallbladder from benign/reactive glandular epithelium can be challenging if based on hematoxylin and eosin–stained sections alone. Immunohistochemical stains for pVHL, maspin, IMP3, and S100P were performed on 82 gallbladder specimens, including adenocarcinoma (n = 33) and nor...
21 CitationsSource
#1Jianhui ShiH-Index: 13
#2Haiyan LiuH-Index: 15
Last. Fan LinH-Index: 25
view all 5 authors...
Summary Our recent study demonstrated the up-regulation of maspin, IMP3, and S100P and downregulation of von Hippel-Lindau gene product (pVHL) in ductal adenocarcinoma of the pancreas. Distinction of adenocarcinoma of the gallbladder from benign/reactive glandular epithelium can be challenging if based on hematoxylin and eosin–stained sections alone. Immunohistochemical stains for pVHL, maspin, IMP3, and S100P were performed on 82 gallbladder specimens, including adenocarcinoma (n = 33) and norm...