Paola Melotti
Thomas Jefferson University
Internal medicinePathologyMolecular biologyAlleleImmunologyIn vivoInflammationCystic fibrosisPseudomonas aeruginosaLungCystic fibrosis transmembrane conductance regulatorAzithromycinSweat testGlutathioneSWEATInterleukin 8MedicineCell cultureBiologyMicrobiologyGastroenterology
115Publications
20H-index
1,837Citations
Publications 114
Newest
The aim of this study was the identification of specific proteomic profiles, related to a restored cystic fibrosis transmembrane conductance regulator (CFTR) activity in cystic fibrosis (CF) leukocytes before and after ex vivo treatment with the potentiator VX770. We used leukocytes, isolated from CF patients carrying residual function mutations and eligible for Ivacaftor therapy, and performed CFTR activity together with proteomic analyses through micro-LC–MS. Bioinformatic analyses of the resu...
Source
#1D. TreggiariH-Index: 1
#2Gloria TridelloH-Index: 24
Last. Paola MelottiH-Index: 20
view all 8 authors...
Sweat chloride (Cl- ) concentration is the gold standard for diagnosing cystic fibrosis (CF). This is however, challenging among patients with borderline values. Previous studies have reported that the sweat Cl- /Na+ ratio may be useful for diagnosing CF; however, little is known about Cl- /K+ and (Cl- +Na+ )/K+ ratios. This study aimed to retrospectively define the most appropriate outcome of the sweat test. Samples of sweat were collected using the Gibson and Cooke method. Cl- , Na+ , and K+ w...
Source
#1D. TreggiariH-Index: 1
#2K. Kleinfelder (University of Verona)H-Index: 1
Last. Paola MelottiH-Index: 20
view all 12 authors...
Abstract Optical measurement of CFTR-dependent sweat secretion stimulated by a beta-adrenergic cocktail (C-phase) vs. CFTR-independent sweat secretion induced by methacholine (M-phase) can discriminate cystic fibrosis (CF) patientts from controls and healthy carriers by the ratio of sweat rate in the C-phase vs. the M-phase (C/M ratio). However, image analysis is experimentally demanding and time-consuming. Here, sweat droplet number (SDN) in the C-phase, corresponding to the number of sweat-sec...
Source
#1Laura Veschetti (University of Verona)H-Index: 3
#2Angela Sandri (University of Verona)H-Index: 5
Last. Maria del Mar Lleò (University of Verona)H-Index: 28
view all 6 authors...
Achromobacter spp. is an opportunistic pathogen that can cause lung infections in patients with cystic fibrosis (CF). Although a variety of mobile genetic elements (MGEs) carrying antimicrobial resistance genes have been identified in clinical isolates, little is known about the contribution of Achromobacter spp. mobilome to its pathogenicity. To provide new insights, we performed bioinformatic analyses of 54 whole genome sequences and investigated the presence of phages, insertion sequences (IS...
Source
#1Paola LeccaH-Index: 7
#2Michela Lecca (fondazione bruno kessler)H-Index: 9
Last. Claudio Sorio (University of Verona)H-Index: 27
view all 8 authors...
We present CORVO (Computing Organoids VOlume in medical images), a tool for calculating the volume of complex time-changing 3D structures from medical videos. In order to identify anisotropies in volume variation over time, CORVO is equipped with a module implementing an advanced regression-based statistical analysis. We tested CORVO for the analysis of the variation of rectal organoids volume, whose anisotropic or missing expansion indicates a pathological state and a non-response to a pharmaco...
Source
#1Rebeca Passarelli Mantovani (University of Verona)H-Index: 1
#2Angela Sandri (University of Verona)H-Index: 5
Last. Maria del Mar Lleò (University of Verona)H-Index: 28
view all 9 authors...
Background Paranasal sinuses act as bacterial reservoirs and contribute to transmitting bacteria to the lower airway of patients with cystic fibrosis (CF). Also, passage of bacteria from the oral cavity to the lungs may occur. Methods We evaluated the presence of Pseudomonas aeruginosa, Staphylococcus aureus, Stenotrophomonas maltophilia, Achromobacter xylosoxidans and Serratia marcescens in sputum and nasal lavage of 59 patients with CF, and also collected saliva and used toothbrushes from 38 o...
1 CitationsSource
#1Bente Aalbers (UU: Utrecht University)H-Index: 3
#2Yasmin Yaakov (HUJI: Hebrew University of Jerusalem)H-Index: 9
Last. I. Bronsveld (UU: Utrecht University)H-Index: 12
view all 11 authors...
Abstract Background 5T polymorphism is a CFTR mutation with unclear clinical consequences: the phenotype varies from healthy individuals to Cystic Fibrosis (CF). The aim of this study was to evaluate if nasal potential difference (NPD) and sweat testing correlate with symptoms and CF diagnosis in 5T patients. Methods 86 patients with 5T who had undergone NPD measurement, were included (6 homozygous (5T/5T), 41 with a PI-CF causing mutation in trans (5T/PI-CF), 11 with a PS-CF causing mutation in...
2 CitationsSource
#1J. ContiH-Index: 1
#2K. KleinfelderH-Index: 1
Last. Paola MelottiH-Index: 20
view all 16 authors...
Source
#1Paola MelottiH-Index: 20
#2Gloria TridelloH-Index: 24
Last. C. D'OrazioH-Index: 2
view all 17 authors...
Source
Source