Krista M.D. La Perle
Memorial Sloan Kettering Cancer Center
GeneLuciferaseInternal medicineEndocrinologyGenomeAntigenCD28Molecular biologyPsychologyProinflammatory cytokineMuscle hypertrophyCD8Elevated plus mazeKinasePolyunsaturated fatty acidHuman genomeModel organismImmunologyIn vivoTranscriptional regulationEpinephrineColitisT cellEicosanoidCardiopulmonary resuscitationProstaglandin EHypothermiaGaussiaHyperplasiaImmunotherapyThyroid-stimulating hormoneChronic lymphocytic leukemiaCytokineCD19Central nervous system diseaseSulfamethoxazoleSevere combined immunodeficiencyHoming (hematopoietic)ErythropoiesisBone marrowNucleic acid metabolismTrimethoprimLinoleic acidGaussia princepsAcute lymphocytic leukemiaPopulationRodent DiseasesNucleoside diphosphate kinase AMammalian embryologyHomologous geneSudden deathMolecular pathogenesisChimeric antigen receptorAnesthesiaAnimal feedBioluminescenceThyroidCentral nervous systemSyntenyGeneticsFlow cytometryBiochemistryMedicineInterleukinIntracellularBiology
Publications 8
#1Claudia Gravaghi (Cornell University)H-Index: 11
#2Krista M.D. La Perle (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 6
Last. Sergio A. Lamprecht (Cornell University)H-Index: 5
view all 7 authors...
Abstract There is great interest in the role of polyunsaturated fatty acids (PUFAs) in promoting ( n -6 class) or inhibiting ( n -3 class) inflammation. Mammalian cells are devoid of desaturase that converts n- 6 to n- 3 PUFAs. Consequently, essential n- 3 fatty acids must be supplied with the diet. We have studied the effect of endogenously produced n -3 PUFAs on colitis development in fat-1 transgenic mice carrying the Caenorhabditis elegans fat-1 gene encoding n -3 desaturase. Colonic cell li...
57 CitationsSource
#1Krista M.D. La Perle (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 6
#2Suzana S. Couto (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 15
Sequencing of the mouse genome has revealed numerous advantages of using the mouse model, compared to other model organisms, to study the molecular pathogenesis of human diseases both the human and mouse genomes contain approximately 30,000 protein-coding genes synteny or conserved gene order on chromosomes in both species is over 90% 40% of human and mouse genes align directly 99% of human genes have homologous gene(s) in the mouse.
1 CitationsSource
#1Edith H. Postel (RU: Rutgers University)H-Index: 6
#2Irene M. Wohlman (RU: Rutgers University)H-Index: 3
Last. Krista M.D. La Perle (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 6
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The ubiquitously expressed nucleoside diphosphate kinases (Nm23/NDPK/Awd) are a large family of multifunctional enzymes implicated in nucleic acid metabolism and in normal and abnormal development. Here, we describe the generation and characterization of NDPK A- and B-deficient (Nme1−/−/Nme2−/−) mice in which >95% of the enzyme activity is eliminated. These mice are undersized, die perinatally, and exhibit a spectrum of hematological phenotypes including severe anemia, impaired maturation of ery...
35 CitationsSource
#1Elmer SantosH-Index: 13
#2Raymond Yeh (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 7
Last. Renier J. Brentjens (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 63
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A research team led by Renier Brentjens offers a new approach to monitor in vivo trafficking of T cells by tagging them for bioluminescence imaging using a membrane-anchored form of the humanized Gaussia luciferase enzyme. The setup provides a high bioluminescent signal and should be useful for studying in vivo T cell function in mouse models of disease investigating mouse or human primary T cells.
118 CitationsSource
#1Renier J. Brentjens (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 63
#2Elmer SantosH-Index: 13
Last. Michel Sadelain (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 103
view all 9 authors...
Purpose: Human T cells targeted to the B cell–specific CD19 antigen through retroviral-mediated transfer of a chimeric antigen receptor (CAR), termed 19z1, have shown significant but partial in vivo antitumor efficacy in a severe combined immunodeficient (SCID)-Beige systemic human acute lymphoblastic leukemia (NALM-6) tumor model. Here, we investigate the etiologies of treatment failure in this model and design approaches to enhance the efficacy of this adoptive strategy. Experimental Design: A...
395 CitationsSource
#1Renier J. Brentjens (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 63
#2Elmer Santos (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 13
Last. Michel Sadelain (MSK: Memorial Sloan Kettering Cancer Center)H-Index: 103
view all 12 authors...
Current approaches to in vivo bioluminescent imaging (BLI) of T cells utilizing luciferase enzymes may be compromised by poor transduction efficiencies in primary T cells, and low photon emissions. Here we describe a novel and widely applicable approach to in vivo BLI of primary T cells utilizing a membrane-anchored form of the naturally secreted Guassia luciferase (GLuc) enzyme, termed exGLuc. We constructed exGLuc by fusion of the GLuc gene to the coding region of the CD8 transmembrane domain....
#1Lotus Y. Altholtz (Cornell University)H-Index: 1
Last. Fred William QuimbyH-Index: 1
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Trimethoprim-sulfamethoxazole (TMP-SMX) medication in the feed or water is commonly administered to immunocompro mised mice to prevent the occurrence of Pneumocystis murina (formerly P. carinii) pneumonia. Therapeutic doses of SMX can cause decreased total and free thyroxine (T4) levels in dogs and thyroid hypertrophy and hyperplasia in mice, rats, and dogs. Our primary objective was to determine whether SMX at doses present in commercially available rodent TMP-SMX feed would pro duce hypothyroi...
12 Citations
#1Gretchen N. Neigh (OSU: Ohio State University)H-Index: 26
#2Julia Kofler (OSU: Ohio State University)H-Index: 7
Last. A. Courtney DeVries (OSU: Ohio State University)H-Index: 22
view all 7 authors...
Advances in medical technology have increased the number of individuals who survive cardiac arrest/cardiopulmonary resuscitation (CPR). This increased incidence of survival has created a population of patients with behavioral and physiologic impairments. We used temperature manipulations to characterize the contribution of central nervous system damage to behavioral deficits elicited by 8 minutes of cardiac arrest/CPR in a mouse model. Once sensorimotor deficits were resolved, we examined anxiet...
31 CitationsSource