Guowen Liu
Jilin University
Internal medicineEndocrinologyProinflammatory cytokineChemistryIn vitroOxidative stressKetosisFatty liverNEFALipid metabolismFatty acid synthaseFatty acidBiochemistryOxidative phosphorylationSignal transductionVery low-density lipoproteinBiologyCell biologyProtein kinase APharmacology
129Publications
27H-index
2,279Citations
Publications 123
Newest
#1Xiaoxia Shi (JLU: Jilin University)H-Index: 8
#2Xinwei LiH-Index: 23
Last. Guowen LiuH-Index: 27
view all 14 authors...
Background/Aims: s-hydroxybutyrate (BHBA) is the major component of ketone bodies in ketosis. Dairy cows with ketosis often undergo oxidative stress. BHBA is related to the inflammation involved in other diseases of dairy cattle. However, whether BHBA can induce inflammatory injury in dairy cow hepatocytes and the potential mechanism of this induction are not clear. The NF-κB pathway plays a vital role in the inflammatory response. Methods: Therefore, this study evaluated the oxidative stress, p...
47 CitationsSource
#1Yu Li (JLU: Jilin University)H-Index: 8
#2Xiaobing Li (JLU: Jilin University)H-Index: 22
Last. Zhe Wang (JLU: Jilin University)H-Index: 26
view all 12 authors...
We have investigated the effect of leptin on gluconeogenesis in the liver. Hepatocytes were cultured and treated with 0, 2.5, 5, 10, 50, 100 ng/mL of leptin in groups I, II, III, IV, V, and VI, respectively. mRNA expression and enzyme activity of pyruvate carboxylase and phosphoenolpyruvate carboxykinase were determined by real-time fluorescence quantitative RT-PCR and biochemical kits, respectively. Compared with group I, mRNA expression of pyruvate carboxylase and phosphoenolpyruvate carboxyki...
4 CitationsSource
#1Hui Chen (JLU: Jilin University)H-Index: 93
#2Liang Zhang (JLU: Jilin University)H-Index: 6
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 16 authors...
Abstract Adiponectin (Ad) plays a crucial role in hepatic lipid metabolism. However, the regulating mechanism of hepatic lipid metabolism by Ad in dairy cows is unclear. Hepatocytes from a newborn female calf were cultured in vitro and treated with different concentrations of Ad and BML-275 (an AMPKα inhibitor). The results showed that Ad significantly increased the expression of two Ad receptors. Furthermore, the phosphorylation and activity of AMPKα, as well as the expression levels and transc...
48 CitationsSource
#1Xinwei Li (JLU: Jilin University)H-Index: 23
#2Hui Chen (JLU: Jilin University)H-Index: 93
Last. Zhe Wang (JLU: Jilin University)H-Index: 26
view all 9 authors...
The effect of acetic acid on hepatic lipid metabolism in ruminants differs significantly from that in monogastric animals. Therefore, the aim of this study was to investigate the regulation mechanism of acetic acid on the hepatic lipid metabolism in dairy cows. The AMP-activated protein kinase (AMPK) signaling pathway plays a key role in regulating hepatic lipid metabolism. In vitro, bovine hepatocytes were cultured and treated with different concentrations of sodium acetate (neutralized acetic ...
50 CitationsSource
#1Zhigang Zhang (NEAU: Northeast Agricultural University)H-Index: 24
#2Jianguo WangH-Index: 10
Last. Xinglin ZhuH-Index: 3
view all 9 authors...
5 CitationsSource
#1Peng Li (Shenyang Agricultural University)H-Index: 14
#2Chen-Chen Wu (JLU: Jilin University)H-Index: 3
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 8 authors...
Abstract In dairy cows, ketosis is an important disease associated with negative energy balance, which leads to low blood glucose levels and high blood nonesterified fatty acid levels. The liver is the most active organ in cows for the metabolism of nonesterified fatty acids. Insulin is an anabolic hormone that plays numerous roles in the metabolism of carbohydrates, lipids, and proteins, as well as being a potent regulator of fatty acid oxidation. In this study, using fluorescent quantitative r...
6 CitationsSource
#1Xinwei Li (JLU: Jilin University)H-Index: 23
#2Yuan Guan (JLU: Jilin University)H-Index: 8
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 8 authors...
Zinc deficiency impairs the hepatic lipid metabolism. Previous studies were focused on the negative effects of zinc deficiency on the hepatic lipid metabolism. A few studies investigated the effects of high zinc levels on the lipid metabolism in hepatocytes. In this study, rat hepatocytes were cultured and treated with different and high concentrations of zinc to investigate the effects of high zinc levels on the lipid synthesis in hepatocytes in vitro. The levels of hepatocytes functional marke...
10 CitationsSource
#1Xinwei Li (JLU: Jilin University)H-Index: 23
#2Xiaobing Li (JLU: Jilin University)H-Index: 22
Last. Zhe Wang (JLU: Jilin University)H-Index: 26
view all 14 authors...
Non-esterified fatty acids (NEFAs) act as signaling molecules involved in regulating genes expression to modulate lipid metabolism. However, the regulation mechanism of NEFAs on lipid metabolism in dairy cows is unclear. The AMP-activated protein kinase (AMPK) signaling pathway plays a key role in regulating hepatic lipid metabolism. In vitro, bovine hepatocytes were cultured and treated with different concentrations of NEFAs and AMPKα inhibitors (BML-275). NEFAs increased AMPKα phosphorylation ...
21 CitationsSource
#1Jianguo Wang (JLU: Jilin University)H-Index: 10
#2Xiaoyan Zhu (JLU: Jilin University)H-Index: 8
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 9 authors...
Strontium stimulates cartilage matrix formation in vitro. However, the mechanisms governing these effects have not yet been extensively reported. In this study, chondrocytes were isolated from rat articular cartilage by enzymatic digestion and cultured for 24–72 h with 1–5 mM strontium. We investigated the effects of different concentrations of strontium on collagen content, type II collagen, insulin-like growth factor (IGF-1) and matrix metalloproteinase (MMP)-13 expression in rat cultured arti...
18 CitationsSource
#1Tao Kong (HAUST: Henan University of Science and Technology)H-Index: 10
#2Hao Xueqin (HAUST: Henan University of Science and Technology)
Last. Jia Sun (KI: Karolinska Institutet)H-Index: 3
view all 10 authors...
The detection of cadmium ions using enzyme-linked immunosorbent assays (ELISA) has been reported by several research groups. Because cadmium ions are too small to stimulate the immune system, high molecular weight immunogens of cadmium are constructed using bifunctional chelators. At present, the most commonly used bifunctional chelator for the preparation of antigens for heavy metal ions is 1-(4-isothiocyanobenzyl) ethylenediamine N,N,N′,N′-tetraacetic acid (ITCBE). However, the price of ITCBE ...
9 CitationsSource