Guowen Liu
Jilin University
Internal medicineEndocrinologyProinflammatory cytokineChemistryIn vitroOxidative stressKetosisFatty liverNEFALipid metabolismFatty acid synthaseFatty acidBiochemistryOxidative phosphorylationSignal transductionVery low-density lipoproteinBiologyCell biologyProtein kinase APharmacology
129Publications
27H-index
2,279Citations
Publications 123
Newest
#1Jihong Dong (JLU: Jilin University)H-Index: 7
#2Juan J. Loor (UIUC: University of Illinois at Urbana–Champaign)H-Index: 68
Last. Xinwei Li (JLU: Jilin University)H-Index: 23
view all 13 authors...
ABSTRACT High blood concentrations of nonesterified fatty acids (NEFA) and altered lipid metabolism are key characteristics of fatty liver in dairy cows. In nonruminants, the mitochondrial membrane protein mitofusin 2 (MFN2) plays important roles in regulating mitochondrial function and intrahepatic lipid metabolism. Whether MFN2 is associated with hepatic lipid metabolism in dairy cows with moderate fatty liver is unknown. Therefore, to investigate changes in MFN2 expression and lipid metabolic...
6 CitationsSource
#1Yiwei Zhu (JLU: Jilin University)H-Index: 9
#2Yuan Guan (JLU: Jilin University)H-Index: 8
Last. Xinwei Li (JLU: Jilin University)H-Index: 23
view all 12 authors...
ABSTRACT Disruption of endoplasmic reticulum (ER) homeostasis, often termed ER stress, is intrinsically linked with perturbation of lipid metabolism in humans and mice. Whether ER homeostasis is affected in cows experiencing fatty liver is unknown. The aim of this study was to investigate the potential role of ER stress in hepatic lipid accumulation in calf hepatocytes and ER stress status in dairy cows with severe fatty liver. In vitro experiments were conducted in which hepatocytes were isolat...
22 CitationsSource
#1Qiushi Xu (JLU: Jilin University)H-Index: 6
#2Xiaobing Li (JLU: Jilin University)H-Index: 22
Last. Xinwei Li (JLU: Jilin University)H-Index: 23
view all 10 authors...
: Ketosis is a major metabolic disorder of high-yielding dairy cows during the transition period. Although metabolic adaptations of the adipose tissue are critical for a successful transition, beyond lipolysis, alterations within adipose tissue during ketosis are not well known. The objective of this study was to investigate the adipose tissue proteome of healthy or ketotic postpartum cows to gain insights into biological adaptations that may contribute to disease outcomes. Adipose tissue biopsy...
6 CitationsSource
#1Chenxu Zhao (JLU: Jilin University)H-Index: 11
#2Yazhou Wang (JLU: Jilin University)H-Index: 3
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
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: Subacute ruminal acidosis (SARA) is characterized by the depression of ruminal pH and an increase in the concentrations of short-chain fatty acids (SCFAs) and lipopolysaccharide (LPS) in the rumen of cows. The onset of SARA was linked to the accumulation of SCFAs. However, the mechanism of SCFAs transport is unknown. The proton-linked monocarboxylate transporter (MCT1) plays a vital role in the transportation of SCFAs. The goal of this study was to elucidate the distribution of MCT1 along the ...
5 CitationsSource
#1Xudong Sun (JLU: Jilin University)H-Index: 7
#2Xiaobing Li (JLU: Jilin University)H-Index: 22
Last. Xinwei Li (JLU: Jilin University)H-Index: 23
view all 13 authors...
ABSTRACT Dairy cows with ketosis display excessive lipolysis in adipose tissue. Heat-shock protein B7 (HSPB7), a small heat-shock protein, plays important roles in mediating cytoprotective responses to oxidative stress in rodent adipose tissue. Accordingly, it is assumed that HSPB7 may also play important roles in the antioxidant response in adipose tissue of ketotic cows. Therefore, the aim of this study is to investigate (1) the redox state of adipose tissue in ketotic cows and (2) the role an...
7 CitationsSource
#1Chenxu Zhao (JLU: Jilin University)H-Index: 11
#2Yazhou Wang (JLU: Jilin University)H-Index: 3
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 10 authors...
: Subacute ruminal acidosis (SARA) can increase the level of inflammation and induce rumenitis in dairy cows. Berberine (BBR) is the major active component of Rhizoma Coptidis, which is a type of Chinese anti-inflammatory drug for gastrointestinal diseases. The purpose of this study was to investigate the anti-inflammatory effects of BBR on lipopolysaccharide (LPS)-stimulated rumen epithelial cells (REC) and the underlying molecular mechanisms. REC were cultured and stimulated with LPS in the pr...
12 CitationsSource
#1Yu Li (AAU: Anhui Agricultural University)H-Index: 8
#2Hongyan Ding (AAU: Anhui Agricultural University)H-Index: 7
Last. Xinwei Li (JLU: Jilin University)H-Index: 23
view all 11 authors...
: The ketotic cows displayed hepatic lipid metabolic disorder and high blood concentration of glucagon. Importantly, adenosine monophosphate-activated protein kinase (AMPK) signaling pathway plays an important role in the hepatic lipid homeostasis. Therefore, the aim of this study was to investigate the effect of glucagon on AMPK pathway and its underlying mechanism on lipid metabolism in cow hepatocytes. Cow hepatocytes were cultured and treated with glucagon and AMPK inhibitor (BML-275). The r...
6 CitationsSource
#1Zhicheng PengH-Index: 9
#2Xiaobing LiH-Index: 22
Last. Xinwei LiH-Index: 23
view all 5 authors...
: Dairy cows with ketosis display severe oxidative stress as well as high blood concentrations of non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHB). Cytochrome P4502E1 (CYP2E1) plays an important role in the induction of oxidative stress. The aim of this study was to investigate CYP2E1 expression and activity in the liver of clinically ketotic cows (in vivo) and the effects of NEFA and BHB on CYP2E1 expression and activity in hepatocytes (in vitro). Dairy cows with clinical ketosis e...
1 CitationsSource
#1Qinghua Deng (IMUN: Inner Mongolia University for Nationalities)H-Index: 8
#1Qinghua Deng (IMUN: Inner Mongolia University for Nationalities)H-Index: 1
Last. Guowen Liu (IMUN: Inner Mongolia University for Nationalities)H-Index: 27
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: Dairy cows with fatty liver or ketosis display decreased insulin sensitivity and defects in the insulin receptor substrate (IRS)/PI3K/AKT signaling pathway. Phosphatase and tensin homolog (PTEN) is a well-known tumor suppressor and also a negative regulator of insulin signaling and peripheral insulin sensitivity. We investigated the hypothesis that PTEN may affect the insulin pathway-mediated hepatic glucose and lipid metabolism in dairy cows. Adenovirus vectors that over-express and silence P...
4 CitationsSource
#1Zheng Li (JLU: Jilin University)H-Index: 7
#2Haihua Feng (JLU: Jilin University)H-Index: 29
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 9 authors...
Abstract Rosmarinic acid (RA) has antioxidation, anticancer, antibacterial, anti-inflammatory and various biological functions. In our study, we aim to evaluate effects of RA on acute liver injury caused by LPS and d -galactosamine ( d -GalN) and its underlying molecular mechanism in mice. Our findings showed that RA could protect C57BL/6 mice from LPS/ d -GalN-induced acute liver injury, which not only reflected on declining aspartate aminotransferase (AST) and alanine aminotransferase (ALT) of...
15 CitationsSource