Guowen Liu
Jilin University
Internal medicineEndocrinologyProinflammatory cytokineChemistryIn vitroOxidative stressKetosisFatty liverNEFALipid metabolismFatty acid synthaseFatty acidBiochemistryOxidative phosphorylationSignal transductionVery low-density lipoproteinBiologyCell biologyProtein kinase APharmacology
129Publications
27H-index
2,279Citations
Publications 126
Newest
The objective of this study was to understand changes of plasma metabolites, hormones, and mRNA level of cytoplasmic phosphoenolpyruvate carboxykinase (PEPCK-C) in liver in spontaneous clinical ketosis; 10 clinically ketotic cows and 10 healthy cows were chosen from the same dairy farm. Eleven blood parameters and liver fat content were measured in all cows, and mRNA levels of PEPCK-C in liver were measured by semi-quantitative reverse transcription (RT) polymerase chain reaction (PCR). In ketot...
2 CitationsSource
#1D. Poisson Paré (Laval University)H-Index: 1
#2D. Song (Laval University)H-Index: 3
Last. Georges Pelletier (Laval University)H-Index: 85
view all 8 authors...
It is known that the steroid sulfatase (STS) and the estrogen sulfotransferase (EST1E1) are commonly expressed in human breast carcinomas. STS and EST1E1 combined action could maintain the equilibrium between sulfated (inactive) and unconjugated (active) estrogens, which might have effects on development of hormone dependent breast cancer. We studied the expression of the STS and EST1E1 in 88 breast carcinomas and 57 adjacent non-malignant tissues by immunohistochemistry. The results were correl...
7 CitationsSource
#1Z. G. Zhang (JLU: Jilin University)H-Index: 3
#2Guowen LiuH-Index: 27
Last. Changming GuoH-Index: 5
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The objective of this study was to examine the association between β-hydroxybutyrate (BHBA) and glucose, calcium (Ca), phosphorus (P), and vitamin C concentrations in dairy healthy and with subclinical ketosis cows during an early lactation period. The blood from 99 healthy cows and 26 sub-clinically ketotic cows within the first two months of lactation was sampled. Serum concentration of BHBA was measured by enzymatic-rate method, glucose by oxidase method, and P by colorimetric method. These t...
11 Citations
#1B. Han (Laval University)H-Index: 1
#2S Li (Laval University)H-Index: 4
Last. Georges Pelletier (Laval University)H-Index: 85
view all 9 authors...
Abstract Estrogens play an important role in the development and progression of breast cancer. 17β-Hydroxysteroid dehydrogenase (17β-HSD) type 2 and type 5 are involved in sex steroid metabolism. 17β-HSD type 2 converts estradiol to estrone while 17β-HSD type 5 converts androstenedione to testosterone. Using immunocytochemistry, we have studied the expression of 17β-HSD type 2 and type 5 in 50 specimens of breast carcinoma and adjacent non-malignant tissues. The results were correlated with the ...
17 CitationsSource
#1Chuang XuH-Index: 8
#2Zhe WangH-Index: 26
Last. Hong you ZhangH-Index: 1
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The objective of the present study was to identify differences in the expression levels of liver proteins between healthy and ketotic cows, establish a liver metabolic interrelationship of ketosis and elucidate the metabolic characteristics of the liver during ketosis. Liver samples from 8 healthy multiparous Hostein cows and 8 ketotic cows were pooled by health status and the proteins were separated by two-dimensional-electrophoresis (2D-E). Statistical analysis of gels was performed using PDQu...
31 CitationsSource
#1D. Song (JLU: Jilin University)H-Index: 3
#2Guowen Liu (JLU: Jilin University)H-Index: 27
Last. Georges PelletierH-Index: 85
view all 11 authors...
Abstract It is known that there is a local biosynthesis of estradiol (E2) in breast carcinoma. The steroidogenic enzymes involved in E2 formation are aromatase which transforms testosterone into E2 and androstenedione into estrone (E1) and reductive 17β-hydroxysteroid dehydrogenases (17β-HSDs) which convert E1 into E2. Using immunocytochemistry, we have studied the expression of aromatase and the three reductive 17β-HSDs 17β-HSD types 1, 7 and 12 in 41 specimens of female human breast carcinoma ...
57 CitationsSource