Guowen Liu
Jilin University
Internal medicineEndocrinologyProinflammatory cytokineChemistryIn vitroOxidative stressKetosisFatty liverNEFALipid metabolismFatty acid synthaseFatty acidBiochemistryOxidative phosphorylationSignal transductionVery low-density lipoproteinBiologyCell biologyProtein kinase APharmacology
129Publications
27H-index
2,279Citations
Publications 126
Newest
#1Chen-Chen Wu (JLU: Jilin University)H-Index: 3
#2Jianguo Wang (JLU: Jilin University)H-Index: 10
Last. Shizheng GaoH-Index: 1
view all 10 authors...
Red-colored bones were first found in Guishan goats in the 1980s, and they were subsequently designated red-boned Guishan goats. However, the difference remains unclear between the bone mineral density (BMD) or elemental composition in bones between red-boned Guishan goats and common Guishan goats. Analysis of femoral bone samples by dual-energy X-ray absorptiometry and inductively coupled plasma optical emission spectrometry revealed an increase in bone mineral density in the femoral diaphysis ...
1 CitationsSource
#1Xiaoxu Wang (JLU: Jilin University)H-Index: 4
#2Xiaobing Li (JLU: Jilin University)H-Index: 22
Last. Zhe Wang (JLU: Jilin University)H-Index: 26
view all 8 authors...
The transition period is a severe challenge to dairy cows. Glucose supply cannot meet demand and body fat is mobilized, potentially leading to negative energy balance (NEB), ketosis, or fatty liver. Propionate produces glucose by gluconeogenesis, which depends heavily on the number and species of microbes. In the present study, we analyzed the rumen microbiome composition of cows in the transition period, cows with ketosis, and nonperinatal cows by terminal restriction fragment length polymorphi...
62 CitationsSource
#1Chen-Chen WuH-Index: 3
#2Xiaobing LiH-Index: 22
Last. Zhe WangH-Index: 26
view all 7 authors...
The objective of our study was to evaluate whether feeding pseudopurpurin affects bone mineral density and bone geometry architecture in rats. Pseudopurpurin was extracted, analyzed and purified using an HLPC-ESI-MS. Rats were given 0% and 0.5% pseudopurpurin powder in their diet. Femurs of rats were examined at 0.5, 1 and 2 months after pseudopurpurin feeding. Compared with rats in the group 0%, the bone mineral density, and the calcium, magnesium, zinc and manganese concentrations in the rats ...
3 CitationsSource
#1Jianguo Wang (JLU: Jilin University)H-Index: 10
#2Xiaoyan Zhu (JLU: Jilin University)H-Index: 8
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
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The major role of insulin-like growth factor-1 (IGF-1) in the liver is to mediate glucose uptake in hepatocytes to synthesize glycogen and maintain blood glucose homeostasis. In this study, to evaluate the role of IGF-1 on gluconeogenesis and nutrient metabolism in dairy cattle, pyruvate carboxylase (PC) and phosphoenolpyruvate carboxykinase (PEPCK) expression and enzyme activity were evaluated in primary cultures of bovine hepatocytes treated with different concentrations of IGF-1 by quantitati...
15 CitationsSource
#1Zhigang ZhangH-Index: 24
#2Xiaobing Li (JLU: Jilin University)H-Index: 22
Last. Ri-He Zhang (JLU: Jilin University)H-Index: 1
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Abstract Primary hepatocytes are commonly used during in vitro studies, but care must be taken with isolation and culture of the cells to ensure their viability. In this study, hepatocytes were isolated from the liver (caudate process) of a newborn calf by the collagenase perfusion and digestion method. The trypan blue exclusion method was used to determine total cell number and the survival rate of hepatocytes, while hepatocyte function was assessed by measuring lactate dehydrogenase, albumin a...
25 CitationsSource
#1Jianguo Wang (JLU: Jilin University)H-Index: 10
#2Xiaoyan Zhu (JLU: Jilin University)H-Index: 8
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 11 authors...
The experiment was performed to evaluate the influence of copper supplementation on insulin-like growth factor-1 (IGF-1) mRNA expression in chondrocytes of newborn pigs. Chondrocytes were isolated and cultured in media containing 15% fetal calf serum supplemented with 0, 15.6, 31.2, and 62.5 μmol/L copper in 90-mm culture plate. After 0, 12, 24, and 48 h, total RNA was isolated from chondrocytes. Then, IGF-1 mRNA expression was determined by semiquantitative RT-PCR. The results showed that the e...
3 CitationsSource
2 Citations
#1Xinwei Li (JLU: Jilin University)H-Index: 23
#2Xiaobing Li (JLU: Jilin University)H-Index: 22
Last. Zhe Wang (JLU: Jilin University)H-Index: 26
view all 9 authors...
Dairy cows experience an increased demand for glucose to support milk production. However, negative energy balance is a common condition in peripartum cows. In response, fat mobilization provides non-esterified fatty acids (NEFAs) for oxidation in the liver to generate ATP. To investigate the effects of NEFAs on gluconeogenesis, the expression and enzyme activity of pyruvate carboxylase (PC) and phosphoenolpyruvate carboxylase (PEPCK) in cultured bovine hepatocytes were evaluated by quantitative...
24 CitationsSource
#1Shixin FuH-Index: 5
#2Qinghua DengH-Index: 8
Last. Guowen LiuH-Index: 27
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Background: Phosphatase and tensin homolog (PTEN) is a potent tumor suppressor gene that also plays a vital role in regulating fatty acid metabolism. Here we attempted to elucidate the role of PTEN in the regulation of fatty acid oxidation and the assembly and secretion of very low density lipoprotein (VLDL) in dairy cow liver. Methods: We transfected primary culture calf hepatocytes with adenovirus-mediated PTEN overexpression vector (AD-GFP-PTEN).PTEN-overexpressing hepatocytes and control hep...
7 CitationsSource
#1Wenyan Yang (JLU: Jilin University)H-Index: 2
#2Jianguo Wang (JLU: Jilin University)H-Index: 10
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 9 authors...
This experiment was conducted to examine the effect of dietary copper supplementation on somatostatin (SS) and growth hormone-releasing hormone (GHRH) mRNA expression levels in the hypothalami of growing pigs. A total of 45 crossbred pigs were randomly assigned to three groups of 15 pigs each; five replicates of three animals comprised each group. Pigs were allocated to diets that contained 5 mg/kg copper (control), 125 mg/kg copper sulfate, or 125 mg/kg copper methionine. At the end of the expe...
27 CitationsSource