Pascal Didier
University of Strasbourg
TautomerAnalytical chemistryBiophysicsGreen fluorescent proteinMolecular biologyNanoparticleFluorescence spectroscopyExcited stateChemistryMaterials scienceIn vitroNucleic acidMoleculeLipid bilayerMembraneChemical engineeringDNABiochemistryFluorescence-lifetime imaging microscopyPhotochemistryBiologyFluorescenceCell biologyCancer cellFörster resonance energy transfer
54Publications
18H-index
1,303Citations
Publications 54
Newest
#1Natalia Grytsyk (UDS: University of Strasbourg)H-Index: 4
#2Damien Cianfarani (UDS: University of Strasbourg)H-Index: 1
Last. Jérémie Léonard (UDS: University of Strasbourg)H-Index: 19
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Interconversions between nucleic acid structures play an important role in transcriptional and translational regulation and also in repair and recombination. These interconversions are frequently promoted by nucleic acid chaperone proteins. To monitor their kinetics, Forster resonance energy transfer (FRET) is widely exploited using ensemble fluorescence intensity measurements in pre-steady-state stopped-flow experiments. Such experiments only provide a weighted average of the emission of all sp...
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#1Eric MoeglinH-Index: 2
#2Dominique DesplancqH-Index: 12
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Histone H2AX phosphorylated at serine 139 (γ-H2AX) is a hallmark of DNA damage, signaling the presence of DNA double-strand breaks and global replication stress in mammalian cells. While γ-H2AX can be visualized with antibodies in fixed cells, its detection in living cells was so far not possible. Here, we used immune libraries and phage display to isolate nanobodies that specifically bind to γ-H2AX. We solved the crystal structure of the most soluble nanobody in complex with the phosphopeptide ...
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#1Dmytro Dziuba (UDS: University of Strasbourg)H-Index: 10
#1Dmytro Dziuba (UDS: University of Strasbourg)H-Index: 1
Last. Yves Mély (UDS: University of Strasbourg)H-Index: 71
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Fluorescent nucleoside analogues (FNAs) are structurally diverse mimics of the natural essentially non-fluorescent nucleosides which have found numerous applications in probing the structure and dynamics of nucleic acids as well as their interactions with various biomolecules. In order to minimize disturbance in the labelled nucleic acid sequences, the FNA chromophoric groups should resemble the natural nucleobases in size and hydrogen-bonding patterns. Isomorphic and expanded FNAs are the two g...
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#1AF Blandin (Harvard University)H-Index: 2
#2Elisabete Cruz da Silva (UDS: University of Strasbourg)H-Index: 5
Last. Maxime Lehmann (UDS: University of Strasbourg)H-Index: 22
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Overexpression of EGFR drives glioblastomas (GBM) cell invasion but these tumours remain resistant to EGFR-targeted therapies such as tyrosine kinase inhibitors (TKIs). Endocytosis, an important modulator of EGFR function, is often dysregulated in glioma cells and is associated with therapy resistance. However, the impact of TKIs on EGFR endocytosis has never been examined in GBM cells. In the present study, we showed that gefitinib and other tyrosine kinase inhibitors induced EGFR accumulation ...
1 CitationsSource
#1Maud Weiss (UDS: University of Strasbourg)H-Index: 3
#2Jiahui Fan (UDS: University of Strasbourg)H-Index: 2
Last. Françoise Pons (UDS: University of Strasbourg)H-Index: 17
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BACKGROUND A positive surface charge has been largely associated with nanoparticle (NP) toxicity. However, by screening a carbon NP library in macrophages, we found that a cationic charge does not systematically translate into toxicity. To get deeper insight into this, we carried out a comprehensive study on 5 cationic carbon NPs (NP2 to NP6) exhibiting a similar zeta (ζ) potential value (from + 20.6 to + 26.9 mV) but displaying an increasing surface charge density (electrokinetic charge, Qek fr...
2 CitationsSource
#1Nario Tomishige (UDS: University of Strasbourg)H-Index: 10
#2Motohide Murate (UDS: University of Strasbourg)H-Index: 17
Last. Toshihide Kobayashi (UDS: University of Strasbourg)H-Index: 62
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Very few proteins are reported to bind specific lipids. Because of the high selectivity and strong binding to specific lipids, lipid-targeting pore forming toxins (PFTs) have been employed to study the distribution of lipids in cell- and model-membranes. Non-toxic and monomeric PFT-derivatives are especially useful to study living cells. In this chapter we highlight sphingomyelin (SM)-binding PFT, lysenin (Lys), its derivatives, and newly identified SM/cholesterol binding protein, nakanori. We d...
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In the quest for the identification of the light emitter(s) responsible for the firefly bioluminescence, the study of oxyluciferin analogues with controlled chemical and electronic structures is of particular importance. In this article, we report the results of our experimental and computational investigation of the pH-dependent absorption spectra characterizing three analogues bound into the luciferase cavity, together with adenosine-monophosphate (AMP). While the analogue microscopic pKa valu...
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#1Jagannath KuchlyanH-Index: 18
Last. Yves Mély (UDS: University of Strasbourg)H-Index: 71
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Thienoguanosine (thG) is an isomorphic guanosine (G) surrogate that almost perfectly mimics G in nucleic acids. To exploit its full potential and lay the foundation for future applications, 20 DNA duplexes, where the bases facing and neighboring thG were systematically varied, were thoroughly studied using fluorescence spectroscopy, molecular dynamics simulations, and mixed quantum mechanical/molecular mechanics calculations, yielding a comprehensive understanding of its photophysics in DNA. In ...
2 CitationsSource
#1Pauline Gosset (UDS: University of Strasbourg)H-Index: 3
#2Grégory Taupier (UDS: University of Strasbourg)H-Index: 4
Last. Pascal Didier (UDS: University of Strasbourg)H-Index: 18
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One of the most characterized bioluminescent reactions involves the firefly luciferase that catalyzes the oxidation of the luciferin producing oxyluciferin in its first excited state. While relaxing to the ground state, oxyluciferin emits visible light with an emission maximum that can vary from green to red. Oxyluciferin exists under six different chemical forms resulting from a keto/enol tautomerization and the deprotonation of the phenol or enol moieties. The optical properties of each chemic...
6 CitationsSource
#1Pascal Didier (UDS: University of Strasbourg)H-Index: 18
#2Jagannath Kuchlyan (UDS: University of Strasbourg)H-Index: 18
Last. Yves Mély (UDS: University of Strasbourg)H-Index: 71
view all 8 authors...
The thienoguanine nucleobase (thGb) is an isomorphic fluorescent analogue of guanine. In aqueous buffer at neutral pH, thGb exists as a mixture of two ground-state H1 and H3 keto–amino tautomers with distinct absorption and emission spectra and high quantum yield. In this work, we performed the first systematic photophysical characterization of thGb as a function of pH (2 to 12). Steady-state and time-resolved fluorescence spectroscopies, supplemented with theoretical calculations, enabled us to...
3 CitationsSource