Xiaoxia Shi
Jilin University
Messenger RNAMatrix metalloproteinasePhosphorylationInternal medicineEndocrinologyPyruvate carboxylasePhosphoenolpyruvate carboxykinaseGene expressionMolecular biologyAlkaline phosphataseProinflammatory cytokineFetusCrossbreedChemistryPeroxisome proliferator-activated receptorExtracellular matrixApoptosisIn vitroImmunologyGluconeogenesisSuperoxide dismutaseOxidative stressHepatocyteKetosisAMP-activated protein kinaseInflammationLactate dehydrogenaseAntioxidantMalondialdehydeGlutathione peroxidaseFatty liverCatalaseGhrelinHydroxyprolineAMPKWeight gainCartilageStearoyl-CoA desaturase-1LeptinMethioninePyrrolidine dithiocarbamateType II collagenZinc deficiencyChondrocyteEnzyme assayCarbohydrate-responsive element-binding proteinDietary CopperFundic GlandSterol Regulatory Element Binding Protein 1Mrna expressionLipid oxidationNf ?b signalingInflammatory responseKetone bodiesCollagen, type I, alpha 1LDL receptorLipid metabolismFatty acid synthaseReactive oxygen speciesp38 mitogen-activated protein kinasesStrontiumZincCopperBiochemistryApolipoprotein EOxidative phosphorylationCell growthMetabolismSignal transductionApolipoprotein BRNAVery low-density lipoproteinBiologyCell biologyProtein kinase A
11Publications
8H-index
224Citations
Publications 11
Newest
#1Xiaoxia Shi (JLU: Jilin University)H-Index: 8
#2Dangdang Li (JLU: Jilin University)H-Index: 3
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 9 authors...
Abstract Dairy cows with fatty liver or ketosis exhibit hyperketonemia, oxidative stress, and a low rate of very low density lipoprotein (VLDL) assembly, and there may be a potential link among these characteristics. Therefore, the objective of this study was to determine the effect of acetoacetic acid (AcAc) on the assembly of VLDL in cow hepatocytes. Cultured cow hepatocytes were treated with different concentrations of AcAc with or without N-acetylcysteine (NAC, an antioxidant). AcAc treatmen...
9 CitationsSource
β-hydroxybutyric acid (BHBA), an important metabolite in β-oxidation, is involved in the development of ketosis in dairy cows. It is known that AMP-activated protein kinase (AMPK) signaling pathway plays an important role in the regulation of lipid metabolism in hepatocytes. In the present study, bovine hepatocytes were treated with BHBA at variable concontrations and Compound C (Cpd C, an AMPK inhibitor) to investigate the effects of BHBA on the AMPK signaling pathway. The results showed that w...
21 CitationsSource
#1Xinwei Li (JLU: Jilin University)H-Index: 23
#2Guowen Liu (JLU: Jilin University)H-Index: 27
Last. Xiaoxia Shi (JLU: Jilin University)H-Index: 8
view all 3 authors...
Abstract Non-esterified fatty acids (NEFAs) are important induction factors of inflammatory responses in some metabolic diseases. High plasma levels of NEFAs and oxidative stress exist in the dairy cows with ketosis. The aim of this study was to investigate whether high levels of NEFAs can induce inflammatory response and the specific molecular mechanism in the hepatocytes of dairy cow. In vitro , primary cultured bovine hepatocytes were treated with different concentrations of NEFAs, PDTC (an N...
57 CitationsSource
#3Yu Li (JLU: Jilin University)H-Index: 11
A high plasma concentration of non-esterified fatty acids (NEFAs) is an important pathogenic factor that leads to ketosis and fatty liver in dairy cows. NEFAs may be associated with oxidative stress in dairy cows with ketosis or fatty liver and the subsequent induction of hepatocyte damage. However, the molecular mechanism of NEFAs-induced oxidative stress and whether NEFAs cause apoptosis of hepatocytes are unclear. Therefore, the aim of this study was to investigate the molecular mechanism of ...
57 CitationsSource
Background/Aims: s-hydroxybutyrate (BHBA) is the major component of ketone bodies in ketosis. Dairy cows with ketosis often undergo oxidative stress. BHBA is related to the inflammation involved in other diseases of dairy cattle. However, whether BHBA can induce inflammatory injury in dairy cow hepatocytes and the potential mechanism of this induction are not clear. The NF-κB pathway plays a vital role in the inflammatory response. Methods: Therefore, this study evaluated the oxidative stress, p...
59 CitationsSource
#1Yu Li (JLU: Jilin University)H-Index: 11
We have investigated the effect of leptin on gluconeogenesis in the liver. Hepatocytes were cultured and treated with 0, 2.5, 5, 10, 50, 100 ng/mL of leptin in groups I, II, III, IV, V, and VI, respectively. mRNA expression and enzyme activity of pyruvate carboxylase and phosphoenolpyruvate carboxykinase were determined by real-time fluorescence quantitative RT-PCR and biochemical kits, respectively. Compared with group I, mRNA expression of pyruvate carboxylase and phosphoenolpyruvate carboxyki...
4 CitationsSource
#1Xinwei Li (JLU: Jilin University)H-Index: 23
#2Yuan Guan (JLU: Jilin University)H-Index: 8
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 8 authors...
Zinc deficiency impairs the hepatic lipid metabolism. Previous studies were focused on the negative effects of zinc deficiency on the hepatic lipid metabolism. A few studies investigated the effects of high zinc levels on the lipid metabolism in hepatocytes. In this study, rat hepatocytes were cultured and treated with different and high concentrations of zinc to investigate the effects of high zinc levels on the lipid synthesis in hepatocytes in vitro. The levels of hepatocytes functional marke...
11 CitationsSource
#1Jianguo Wang (JLU: Jilin University)H-Index: 10
#2Xiaoyan Zhu (JLU: Jilin University)H-Index: 8
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
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Strontium stimulates cartilage matrix formation in vitro. However, the mechanisms governing these effects have not yet been extensively reported. In this study, chondrocytes were isolated from rat articular cartilage by enzymatic digestion and cultured for 24–72 h with 1–5 mM strontium. We investigated the effects of different concentrations of strontium on collagen content, type II collagen, insulin-like growth factor (IGF-1) and matrix metalloproteinase (MMP)-13 expression in rat cultured arti...
18 CitationsSource
#1Wenyan Yang (JLU: Jilin University)H-Index: 2
#2Jianguo Wang (JLU: Jilin University)H-Index: 10
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 10 authors...
This experiment was conducted to examine the effect of dietary copper supplementation on ghrelin mRNA expression level in the fundic gland of growing pigs. A total of 45 crossbred pigs were randomly assigned to three groups of 15 pigs, five replicates of three animals comprised each group. Pigs were allocated to diets that contained 5 mg/kg copper (as the control group), 125 mg/kg copper sulfate, or 125 mg/kg copper methionine. At the end of the experiment, five pigs were selected at random from...
11 CitationsSource