Gianfranco Pintus
University of Sassari
Endothelial stem cellInternal medicineEndocrinologyMolecular biologyChemistryProtein kinase COxidative stressAntioxidantVascular smooth muscleMitochondrionCapillary electrophoresisProgrammed cell deathChromatographyReactive oxygen speciesCancer researchBiochemistrySignal transductionMedicineBiologyCell biologyPharmacology
Publications 138
#1Anna Maria PosadinoH-Index: 19
#2A CossuH-Index: 14
Last. Gianfranco PintusH-Index: 32
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#1Maria Letizia Taddei (UniFI: University of Florence)H-Index: 32
#2Lorenzo Cavallini (UniFI: University of Florence)H-Index: 8
Last. Paola Chiarugi (UniFI: University of Florence)H-Index: 67
view all 12 authors...
We focused our interest on senescent human-derived fibroblasts in the progression of prostate cancer. Hypoxic senescent fibroblasts promote prostate cancer aggressiveness by inducing epithelial to mesenchymal transition (EMT) and by secreting energy-rich compounds to support cancer cell growth. Hypoxic senescent fibroblasts additionally increase: i) the recruitment of monocytes and their M2-macrophage polarization, ii) the recruitment of bone marrow-derived endothelial precursor cells, facilitat...
71 CitationsSource
#1Angelo Zinellu (University of Sassari)H-Index: 31
#2Salvatore Sotgia (University of Sassari)H-Index: 25
Last. Gianfranco Pintus (University of Sassari)H-Index: 32
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Abstract The natural antioxidant-associated biological responses appear contradictory since biologically active dosages registered in vitro experiments are considerably higher if compared to concentrations found in vivo. The recent research indicates that natural antioxidants, including the major catechins of green tea epicatechin (EC), epigallocatechin (EGC), epicatechingallate (ECG) and epigallocatechingallate (EGCG) form non-covalent complexes with albumin, a crucial aspect that may modulate ...
20 CitationsSource
#1Salvatore Sotgia (University of Sassari)H-Index: 25
#2Elisabetta Pisanu (University of Sassari)H-Index: 9
Last. Angelo Zinellu (University of Sassari)H-Index: 31
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A new efficient and sensitive precolumn hydrophilic interaction ultra-performance liquid chromatography (HILIC-UPLC) method was established for the quantitative determination of L-ergothioneine (ERT) in milk. After derivatization of ERT with 7-diethylamino-3-[4-(iodoacetamido)phenyl]-4-methylcoumarin, chromatographic separation was achieved in a fairly short time, less than 5 min, on a 100 × 2.1 mm Waters Cortecs UPLC HILIC 1.6-μm column, by using a mixture of 30 mmol/L ammonium acetate/acetonit...
10 CitationsSource
#1Mauro Forteschi (University of Sassari)H-Index: 6
#2Salvatore Sotgia (University of Sassari)H-Index: 25
Last. Ciriaco Carru (University of Sassari)H-Index: 38
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A new capillary electrophoresis method to measure human blood plasma arginine and citrulline levels in a single run without derivatization was established. After adding homoarginine as internal standard, plasma proteins were removed by a 90:10 v/v acetonitrile/ammonia mixture. Arginine and citrulline were detected by an ultraviolet detector at 190 nm and separated in 11.65 and 20.43 min, respectively, by using a 75 mmol/L Tris phosphate solution at pH 1.2 as a background electrolyte. Limits of d...
11 CitationsSource
#1Francesco Boin (Johns Hopkins University)H-Index: 24
#2Gian Luca Erre (University of Sassari)H-Index: 17
Last. Gianfranco Pintus (University of Sassari)H-Index: 32
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Pulmonary arterial hypertension is a major complication of systemic sclerosis. Although oxidative stress, intima hyperplasia and a progressive vessel occlusion appear to be clearly involved, the fine molecular mechanisms underpinning the onset and progression of systemic sclerosis-associated pulmonary arterial hypertension remain largely unknown. Here we shows for the first time that an increase of NADPH-derived reactive oxygen species production induced by sera from systemic sclerosis patients ...
22 CitationsSource
#1Mingyi Wang (NIH: National Institutes of Health)H-Index: 28
#2Gianfranco Pintus (NIH: National Institutes of Health)H-Index: 32
Last. Edward G. Lakatta (NIH: National Institutes of Health)H-Index: 166
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Collagen deposition, a hallmark of arterial aging that resembles post-injury arterial restenosis, is perpetrated by angiotensin II (Ang II) signaling in arterial wall. Collagen aggregation at sites of arterial injury is regulated by the coordinated signaling of pro-fibrotic TGF-β1 and anti-fibrotic vasorin within VSMCs. The Ang II/TGF-β1/vasorin signaling relationship within VSMCs with aging, however, remains unknown. In vivo studies in old vs. young FXBN rats show that aortic transcription and ...
#1Salvatore Sotgia (University of Sassari)H-Index: 25
#2Angelo Zinellu (University of Sassari)H-Index: 31
Last. Mark McEvoy (University of Newcastle)H-Index: 51
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Background: Despite the increasing interest towards the biological role of L-ergothioneine, little is known about the serum concentrations of this unusual aminothiol in older adults. We addressed this issue in a representative sample of communitydwelling middle-aged and older adults. Methods: Body mass index, estimated glomerular filtration rate, serum concentrations of L-ergothioneine, taurine, homocysteine, cysteine, glutathione, cysteinylglycine, and glutamylcysteine were evaluated in 439 sub...
20 CitationsSource
#1Salvatore Sotgia (University of Sassari)H-Index: 25
#2Elisabetta Pisanu (University of Sassari)H-Index: 9
Last. Angelo Zinellu (University of Sassari)H-Index: 31
view all 8 authors...
Two sensitive and reproducible capillary electrophoresis and high-performance liquid chromatography-fluorescence procedures were established for quantitative determination of L-egothioneine in plasma. After derivatization of L-ergothioneine with 5-iodoacetamidofluorescein, the separation was carried out by HPLC on an ODS-2 C-18 sperisorb column by using a linear gradient elution and by HPCE on an uncoated fused silica capillary, 50 µm id, and 60 cm length. The methods were validated and found to...
17 CitationsSource
#1Tania Fiaschi (UniFI: University of Florence)H-Index: 27
#2Elisa Giannoni (UniFI: University of Florence)H-Index: 52
Last. Paola Chiarugi (UniFI: University of Florence)H-Index: 67
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Extracellular acidification, a mandatory feature of several malignancies, has been mainly correlated with metabolic reprogramming of tumor cells toward Warburg metabolism, as well as to the expression of carbonic anydrases or proton pumps by malignant tumor cells. We report herein that for aggressive prostate carcinoma, acknowledged to be reprogrammed toward an anabolic phenotype and to upload lactate to drive proliferation, extracellular acidification is mainly mediated by stromal cells engaged...
98 CitationsSource