Kazuyoshi Kumagai
Daiichi Sankyo
Internal medicineEndocrinologyPathologyVimentinMolecular biologyAntibodyProinflammatory cytokineTumor necrosis factor alphaChemistryApoptosisImmunologyImmunohistochemistryLiver injuryLungCycloheximideCytokineKupffer cellCytokeratinAtypiaToxicityProgrammed cell deathNecrosisHistopathologyMedicineChromogranin ABiologyPharmacology
18Publications
7H-index
235Citations
Publications 18
Newest
#1Takayasu Moroki (OPC: Otsuka Pharmaceutical)
#2Saori Matsuo (Chugai Pharmaceutical Co.)H-Index: 8
Last. Kiyokazu Ozaki (Setsunan University)H-Index: 19
view all 9 authors...
With the aim of sharing information about the technical aspects of immunohistochemistry (IHC) and facilitating the selection of suitable antibodies for histopathological examination, this technical report describes the results of a questionnaire distributed during the period of 2018 to 2019 among members of the Conference on Experimental Animal Histopathology. Additionally, it describes the immunological properties and supplier details (clone, supplier, catalog number, species reactivity, etc.) ...
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#1Kazuyoshi Kumagai (Daiichi Sankyo)H-Index: 7
#2Tetsuo Aida (Daiichi Sankyo)H-Index: 6
Last. Kazuhiko Mori (Daiichi Sankyo)H-Index: 9
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Trastuzumab deruxtecan (T-DXd: DS-8201a) is an anti-human epidermal growth factor receptor 2 (HER2) Ab-drug conjugated with deruxtecan (DXd), a derivative of exatecan. The objective of this study was to characterize T-DXd-induced lung toxicity in cynomolgus monkeys. Trastuzumab deruxtecan was injected i.v. into monkeys once every 3 weeks for 6 weeks (10, 30, and 78.8 mg/kg) or for 3 months (3, 10, and 30 mg/kg). To evaluate the involvement of DXd alone in T-DXd-induced toxicity, DXd monohydrate ...
2 CitationsSource
#1Eri Hamamura-Yasuno (Daiichi Sankyo)H-Index: 1
#2Takuma Iguchi (Daiichi Sankyo)H-Index: 5
Last. Kazuhiko Mori (Daiichi Sankyo)H-Index: 9
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MAS-related G protein coupled receptor-X2 (MRGPRX2), expressed in human mast cells, is associated with drug-induced pseudo-allergic reactions. Dogs are highly susceptible to drug-induced anaphylactoid reactions caused by various drugs; however, the distribution and physiological function of canine MRGPR family genes, including MRGPRX2, remain largely unknown. In the present study, we clarified the distribution of dog MRGPR family genes by real-time quantitative PCR and in situ hybridisation. We ...
2 CitationsSource
#1Tomohiro Shirayanagi (Chiba University)H-Index: 1
#2Shigeki Aoki (Chiba University)H-Index: 7
Last. Kousei Ito (Chiba University)H-Index: 29
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The interaction of human leukocyte antigen (HLA) with specific drugs is associated with delayed-type hypersensitivity reactions, which cause severe cutaneous toxicity. Such interactions induce structural alterations in HLA complexes via several different mechanisms such as the hapten theory, p-i concept, and altered peptide repertoire model, leading to the activation of cytotoxic T cells. To date, comprehensive detection of such structural alterations in preclinical studies has been difficult. H...
1 CitationsSource
#1Takanori Maejima (Daiichi Sankyo)H-Index: 6
#2Kazuyoshi Kumagai (Daiichi Sankyo)H-Index: 7
Last. Kazuhiko Mori (Daiichi Sankyo)H-Index: 9
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: A 40-week-old male spontaneous diabetic Torii rat, an animal model of type 2 diabetes mellitus, was found to have marked urinary calculi with hematuria in the urinary bladder on necropsy. Histological findings in the urinary bladder included a papillary growth pattern with a fibrovascular stroma without atypia. Fine granular materials in the bladder lumen were positive for Von Kossa staining but negative for periodic acid-Schiff or Gram staining, indicating no apparent bacterial infection in t...
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#1Kyohei Yasuno (Daiichi Sankyo)H-Index: 1
#2Kazuyoshi Kumagai (Daiichi Sankyo)H-Index: 7
Last. Kazuhiko Mori (Daiichi Sankyo)H-Index: 9
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Diacylglycerol acyltransferase 1 (DGAT1) catalyzes the final step in triglyceride synthesis. Since Dgat1-/- mice fed a high-fat diet (HFD) are resistant to hepatic steatosis, DGAT1 inhibitors are expected to have antifatty liver effects. To evaluate the hepatic effects of DS-7250, a selective DGAT1 inhibitor, vehicle or 10 mg/kg of DS-7250 was administered orally to male Fisher 344 (F344) and Zucker fatty (ZF) rats fed a standard diet or HFD for 14 or 28 days. ZF rats showed slight hepatic steat...
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#1Makoto ShiraiH-Index: 4
#2Kiyonori KaiH-Index: 5
Last. Atsushi SanbuisshoH-Index: 11
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A nodule was observed in the adrenal medulla of a twenty-week-old male Wistar Hannover rat. The nodule was predominantly (over 80%) composed of neural components, with ganglion cells scattered in sparse supporting tissue containing nerve fibers and Schwann cells. In the peripheral area of the tumor, atypical chromaffin cells were also observed. Accumulation of eosinophilic serous fluid was also noted in the stromal tissue. There were neither mitotic figures in the ganglion cells nor necrotic foc...
1 CitationsSource
#1Makoto Shirai (Daiichi Sankyo)H-Index: 4
#2Takanori Maejima (Daiichi Sankyo)H-Index: 6
Last. Atsushi Sanbuissho (Daiichi Sankyo)H-Index: 11
view all 8 authors...
Multiple whitish nodules in the thoracic cavity at the site of the thymus were observed in a 101-week-old male ICR mouse. In a histopathological examination, the neoplastic cells were predominantly fusiform in shape and proliferated in sarcomatoid growth patterns. Some neoplastic cells showed epithelial growth patterns, such as the ductal structures. Mitotic figures were frequently seen, and small necrotic foci and invasion to adjacent thoracic organs were noted. In Alcian blue staining, bluish ...
3 CitationsSource
#1Kazuyoshi Kumagai (Daiichi Sankyo)H-Index: 7
#2Kazumi Ito (Daiichi Sankyo)H-Index: 11
Last. Sunao Manabe (Daiichi Sankyo)H-Index: 17
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Cycloheximide (CHX)-induced liver injury in rats has been characterized by hepatocellular apoptosis and necrosis. We previously reported that Kupffer cell inactivation causes a reduction of IL-10 production, resulting in the exacerbation of CHX-induced liver injury. In this study, we directly evaluate the role of IL-10 in liver injury by a pretreatment with anti-IL-10 neutralizing antibody (IL-10Ab). Rats were given goat IgG or IL-10Ab before being treated with CHX (CHX group or IL-10Ab/CHX grou...
7 CitationsSource
#1Kazunori Fujimoto (Daiichi Sankyo)H-Index: 9
#2Kazuyoshi KumagaiH-Index: 7
Last. Sunao ManabeH-Index: 17
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Recently, it was reported that the intraperitoneal administration of 30 mg/kg/day troglitazone to heterozygous superoxide dismutase 2 gene knockout (Sod2+/−) mice for twenty-eight days caused liver injury, manifested by increased serum ALT activity and hepatic necrosis. Therefore, we evaluated the reproducibility of troglitazone-induced liver injury in Sod2+/− mice, as well as their validity as an animal model with higher sensitivity to mitochondrial toxicity by single-dose treatment with acetam...
82 CitationsSource