Gabriela Québatte
University of Basel
ParticleBiophysicsIsothermal titration calorimetryOrganic chemistryLiposomeBase pairChemistryBilayerMaterials scienceIn vitroMelittinCalcium carbonateHeparan sulfatePhospholipidDrug deliveryDrugBinding constantMembraneChemical engineeringBiochemistryPorosity
11Publications
6H-index
76Citations
Publications 11
Newest
#1Maryam Farzan (University of Basel)H-Index: 4
#2Gabriela Québatte (University of Basel)H-Index: 6
Last. Maxim Puchkov (University of Basel)H-Index: 14
view all 7 authors...
Despite the wide-spread use of liposomal drug delivery systems, application of these systems for oral purposes is limited due to their large-scale formulation and storage issues. Proliposomes are one of the formulation approaches for achieving solid powders that readily form liposomes upon hydration. In this work, we investigated a dry powder formulation of a model low-soluble drug with phospholipids loaded in porous functionalized calcium carbonate microparticles. We characterized the liposome ...
1 CitationsSource
#1Leonie Wagner-Hattler (University of Basel)H-Index: 1
#2Gabriela Québatte (University of Basel)H-Index: 6
Last. Maxim Puchkov (University of Basel)H-Index: 14
view all 7 authors...
Abstract Uniform drug distribution within fast disintegrating tablets is a key quality measure to ensure a reliable, steady, and targeted release of the contained active pharmaceutical ingredients. In this work, the drug particle distribution in mini-tablets was studied with synchrotron phase contrast X-ray microtomography. Mini-tablets had a weight of 9.5 mg and a drug load from 2.5% to 20%. Moxidectin, a drug used for treatment of parasitic infections, was used as a model compound. Drug conten...
2 CitationsSource
#1Dominik Witzigmann (University of Basel)H-Index: 16
#2Philipp Uhl (University Hospital Heidelberg)H-Index: 7
Last. Jörg Huwyler (University of Basel)H-Index: 49
view all 16 authors...
: Active targeting and specific drug delivery to parenchymal liver cells is a promising strategy to treat various liver disorders. Here, we modified synthetic lipid-based nanoparticles with targeting peptides derived from the hepatitis B virus large envelope protein (HBVpreS) to specifically target the sodium-taurocholate cotransporting polypeptide (NTCP; SLC10A1) on the sinusoidal membrane of hepatocytes. Physicochemical properties of targeted nanoparticles were optimized and NTCP-specific, lig...
11 CitationsSource
#1Maryam Farzan (University of Basel)H-Index: 4
#2Roger Roth (University of Basel)H-Index: 3
Last. Maxim Puchkov (University of Basel)H-Index: 14
view all 6 authors...
Accurate analysis of intraparticle distribution of substances within porous drug carriers is important to optimize loading and subsequent processing. Mercury intrusion porosimetry, a common technique used for characterization of porous materials, assumes cylindrical pore geometry, which may lead to misinterpretation. Therefore, imaging techniques such as focused ion beam scanning electron microscopy (FIB-SEM) help to better interpret these results. The purpose of this study was to investigate th...
8 CitationsSource
#1Dominik Witzigmann (University of Basel)H-Index: 16
#2Philipp Uhl (University Hospital Heidelberg)H-Index: 7
Last. Jörg Huwyler (University of Basel)H-Index: 49
view all 16 authors...
Source
#1Helene Kettiger (University of Basel)H-Index: 8
#2Gabriela Québatte (University of Basel)H-Index: 6
Last. Jörg Huwyler (University of Basel)H-Index: 49
view all 4 authors...
Silica nanoparticles (SNPs) are widely used for biomedical applications. However, their parenteral administration may induce hemolysis. Molecular mechanisms leading to this effect are still controversially discussed. We therefore used a combination of biophysical techniques to investigate the interaction of hemolytic and non-hemolytic SNPs with model phospholipid membranes. Methods Interaction of SNPs with membranes was studied using a dye-leakage assay, dynamic light scattering (DLS), isotherma...
27 CitationsSource
#1Philip Grossen (University of Basel)H-Index: 8
#2Gabriela Québatte (University of Basel)H-Index: 6
Last. Jörg Huwyler (University of Basel)H-Index: 49
view all 6 authors...
Nanoparticles are increasingly used to implement drug targeting strategies. In the present study, solid-sphere nanoparticles (SNPs) made of poly(ethylene glycol)-b-poly(ź-caprolactone) (PEG-b-PCL) were covalently linked to a monoclonal antibody (83-14źmAb) targeted against the human insulin receptor that is highly expressed on human brain microvascular endothelial cells. Resulting targeted SNPs were characterized using transmission electron microscopy (TEM), cryo-TEM, dynamic light scattering, a...
9 CitationsSource
#1Anacelia Ríos Quiroz (University of Basel)H-Index: 1
#2Gabriela Québatte (University of Basel)H-Index: 6
Last. Michael AdlerH-Index: 11
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Although light obscuration is the “gold standard” for subvisible particle measurements in biopharmaceutical products, the current technology has limitations with respect to the detection of translucent proteinaceous particles and particles of sizes smaller and around 2 μm. Here, we describe the evaluation of a modified light obscuration sensor utilizing a novel measuring mode. Whereas standard light obscuration methodology monitors the height (amplitude) of the signal, the new approach monitors ...
6 CitationsSource
#1Gabriela Québatte (University of Basel)H-Index: 6
#2Eric A. Kitas (Hoffmann-La Roche)H-Index: 19
Last. Joachim Seelig (University of Basel)H-Index: 96
view all 3 authors...
Abstract Melittin is an amphipathic peptide which has received much attention as a model peptide for peptide–membrane interactions. It is however not suited as a transfection agent due to its cytolytic and toxicological effects. Retro-inverso-melittin, when covalently linked to the lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (riDOM), eliminates these shortcomings. The interaction of riDOM with phospholipid membranes was investigated with circular dichroism (CD) spectroscopy, dynamic ligh...
10 CitationsSource
#1Joachim Seelig (University of Basel)H-Index: 96
#2Gabriela Québatte (University of Basel)H-Index: 6
Last. Eric A. Kitas (Hoffmann-La Roche)H-Index: 19
view all 3 authors...
riDOM (retro-inverso-dioleoyl-melittin) is a cell penetrating peptide with excellent transporter properties for DNA. It is a chimeric molecule where ri-melittin is fused to dioleoylphosphoethanolamine. The physical-chemical properties of riDOM in solution and in the presence of DNA, heparan sulfate and lipid membranes were investigated with spectroscopic and thermodynamic methods. riDOM in solution forms nanoparticles with a diameter of ∼13 nm, composed of about 220 - 270 molecules. riDOM binds ...
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