Wei Cheng
Chongqing Medical University
Linear rangeSurface plasmon resonanceDetection limitBiophysicsAptamerMolecular biologyBiosensorNanotechnologyChemistryMaterials scienceBiotinylationCombinatorial chemistryNucleic acidStreptavidinHeminElectrochemistryDNABiochemistryRolling circle replicationDeoxyribozyme
93Publications
28H-index
2,206Citations
Publications 93
Newest
#1Guozhen Tian (HMU: Hainan Medical University)H-Index: 1
#2Decai Zhang (CQMU: Chongqing Medical University)H-Index: 10
Last. Qianfeng Xia (HMU: Hainan Medical University)H-Index: 7
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Abstract null null The extraordinary genome-editing tool CRISPR/Cas12a has also been utilized as a powerful sensing technology owing to its highly-specificity and isothermal signal amplification. Nevertheless, the widespread application of Cas12a-based sensing methods in nucleic acid detection is limited by the targeting range and high undesired background. Herein, we established a universal Cas12a-based nucleic acid sensing strategy by using proximity extension and transcription-unleashed self-...
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Gliomas are the most common type of primary brain tumor, yet the prognosis for glioma patients remains poor. Mutations in the promoter region of the telomerase reverse transcriptase gene (TERTp) are associated with diagnosis and poor prognosis in gliomas. Here, we developed a precise and rapid Sanger sequencing assay to screen or TERTp mutations. We established the Sanger sequencing approach for the detection of TERTp mutations based on human glioma cell lines U251 and assessed the analytical va...
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#1Lulu Xu (CQMU: Chongqing Medical University)H-Index: 6
#2Zheng Zhou (Chongqing University)
Last. Fangzhou Song (CQMU: Chongqing Medical University)H-Index: 2
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Abstract Efficient and multiple analysis of receptor protein dimers is highly necessary, due to their important role in in the occurrence and development of cancer. Herein, we report a turn-on strategy to visualize human epidermal growth factor receptor (HER) dimers on cell surfaces. By taking advantages of specific aptamer recognition and proximity-induced fluorescence activation of DNA-templated sliver nanoclusters (DNA/AgNCs) by guanine (G)-rich sequence, we attached the two kind of DNA/AgNCs...
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#1Lutan Zhang (CQMU: Chongqing Medical University)
#1Lutan Zhang (CQMU: Chongqing Medical University)H-Index: 4
Last. Wei Cheng (CQMU: Chongqing Medical University)H-Index: 28
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Papillary thyroid carcinoma (PTC) is the most common thyroid cancer with high incidence in endocrine tumors, which emphasizes the significance of accurate diagnostics. Still, the commonly used cytological method (fine-needle aspiration (FNA) cytology) and molecular diagnostic methods (such as PCR and sequencing) are limited in terms of diagnostic time, sensitivity, and user-friendliness. In this study, we introduce a novel Zip recombinase polymerase amplification (Z-RPA) strategy to efficiently ...
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#2Tiantian YangH-Index: 4
Last. Wei ChengH-Index: 28
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Abstract As one of the crucial factors associated with human life span and cancer progression, telomerase is regarded as an emerging biomarker for cancer diagnosis. Therefore, a facile, rapid and sensitive approach for telomerase activity detection with point-of-care (POC) diagnosis potential is in great demands. Herein, an all-in-one telomerase activity detection assay was established based on the telomere synthesis activated CRISPR-Cas12a system. A telomerase extension reaction generated telom...
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#1Xiaoxue Cheng (CQMU: Chongqing Medical University)H-Index: 1
#2Yurong Yan (CQMU: Chongqing Medical University)H-Index: 16
Last. Wei Cheng (CQMU: Chongqing Medical University)H-Index: 28
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Abstract Cas12a shows great promise in DNA sensing applications due to its target-triggered collateral trans-cleavage activity. But the cleavage effect towards probes modified on nanomaterials is less understood. In this work, an analogy analysis is performed to explore the cleavage properties of Cas12a system on the surface of nanomaterials by using gold nanoparticles (AuNPs) and graphene oxide (GO). The fluorescence of FAM-tagged probes is quenched by nanomaterials due to fluorescence resonanc...
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#1Lulu XuH-Index: 6
#2Jiaxin DuanH-Index: 1
Last. Wei ChengH-Index: 28
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Abstract Rolling circle amplification (RCA) is an efficient enzymatic isothermal reaction that using circular probe as a template to generate long tandem single-stranded DNA or RNA products under the initiation of short DNA or RNA primers. As a simplified derivative of natural rolling circle replication which synthesizes copies of circular nucleic acids molecules such as plasmids, RCA amplifies the circular template rapidly without thermal cycling and finds various applications in molecular biol...
1 CitationsSource
#1Lina Kang (CQMU: Chongqing Medical University)
#2Jiaxin Duan (CQMU: Chongqing Medical University)H-Index: 1
Last. Wei Cheng (CQMU: Chongqing Medical University)H-Index: 28
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Abstract Simple, sensitive and specific detection of the transcription level of BCR-ABL1 mRNA possesses vital clinical significance in diagnosis and treatment of chronic myeloid leukemia (CML). In this study, an innovative fluorescence biosensing methodology has been developed for sensitive and specific detection of BCR-ABL1 mRNA by integrating high-efficiency of exponential transcription and superior catalytic performance of DNA-grafted hemin. Exponential transcription was triggered by BCR-ABL1...
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#1Ying Zheng (CQMU: Chongqing Medical University)H-Index: 1
#2Siqiao Li (CQMU: Chongqing Medical University)H-Index: 3
Last. Shijia Ding (CQMU: Chongqing Medical University)H-Index: 28
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Despite the ever-growing endangerment caused by the multidrug resistance (MDR) of bacteria, the development of effective antibacterial materials still remains a global challenge. Current antibiotic therapies cannot simultaneously inactivate bacteria and accelerate wound healing. This study aimed to originally separate the intercalation of MnO3+ and the oxidation processes to synthesize epoxy-rich graphene oxide (erGO) nanofilms via an eco-friendly synthetic route, which possessed low density and...
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#1Heye Lv (CQMU: Chongqing Medical University)H-Index: 2
#2Anyi Chen (CQMU: Chongqing Medical University)H-Index: 1
Last. Wei Cheng (CQMU: Chongqing Medical University)H-Index: 28
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Herein, highly efficient deoxyribonucleic acid (DNA) walking on electrode surfaces was realized by regulating DNA tracks, which was applied to construct an ultrasensitive electrochemiluminescent (ECL) biosensor for BCR/ABL fusion gene detection. The well-regulated DNA tracks were constructed via supersandwich hybridization chain reaction of two DNA strands (L1 and L2) to generate periodic linear dsDNA concatemers, where an exposed L1 domain closed with blocking strands (BS). The prepared DNA tra...
1 CitationsSource