Jianwu Dai
Chinese Academy of Sciences
AnatomyBiomedical engineeringNeural stem cellPathologyRegeneration (biology)ChemistryMaterials scienceMesenchymal stem cellIn vivoBasic fibroblast growth factorSpinal cord injuryAngiogenesisSpinal cordStem cellTransplantationTissue engineeringMedicineBiologyScaffoldCell biology
305Publications
69H-index
10.8kCitations
Publications 280
Newest
#1Muya Shu (CAS: Chinese Academy of Sciences)H-Index: 2
#2Xiaoyu Xue (CAS: Chinese Academy of Sciences)H-Index: 3
Last. Jianwu Dai (CAS: Chinese Academy of Sciences)H-Index: 69
view all 17 authors...
Neural stem cells (NSCs) in the spinal cord hold great potential for repair after spinal cord injury (SCI). The ependyma in the central canal (CC) region has been considered as the NSCs source in the spinal cord. However, the ependyma function as NSCs after SCI is still under debate. We used Nestin as a marker to isolate potential NSCs and their immediate progeny, and characterized the cells before and after SCI by single-cell RNA-sequencing (scRNA-seq). We identified two subgroups of NSCs: the ...
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#1Wen Yin (CSU: Central South University)H-Index: 4
#2Weiwei Xue (CAS: Chinese Academy of Sciences)H-Index: 5
Last. Jianwu Dai (CAS: Chinese Academy of Sciences)H-Index: 69
view all 19 authors...
Scar tissue removal combined with biomaterial implantation is considered an effective measure to repair chronic transected spinal cord injury (SCI). However, whether more scar tissue removal surgeries could affect the treatment effects of biomaterial implantation still needs to be explored. In this study, we performed the first scar tissue removal surgery in the 3rd month and the second in the 6th month after completely removing 1 cm of spinal tissue in canines. We found that Taxol-modified line...
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#1Weiwei Xue (CAS: Chinese Academy of Sciences)H-Index: 5
#2Haipeng Zhang (CAS: Chinese Academy of Sciences)H-Index: 2
Last. Jianwu Dai (CAS: Chinese Academy of Sciences)H-Index: 69
view all 13 authors...
BACKGROUND Microtubule-stabilizing agents have been demonstrated to modulate axonal sprouting during neuronal disease. One such agent, Epothilone D, has been used to treat spinal cord injury (SCI) by promoting axonal sprouting at the lesion site after SCI. However, the role of Epothilone D in the differentiation of neural stem cells (NSCs) in SCI repair is unknown. In the present study, we mainly explored the effects and mechanisms of Epothilone D on the neuronal differentiation of NSCs and reve...
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#1Huang Lei (USTC: University of Science and Technology of China)
#2Yun Chu (USTC: University of Science and Technology of China)
Last. Jianwu Dai (CAS: Chinese Academy of Sciences)H-Index: 69
view all 7 authors...
Chitosan (CS) hydrogels have been widely used throughout basic tissue engineering and regenerative medicine research and it is very desirable to develop advanced CS materials with superior mechanical and topographical properties for more extensive applications. Herein, we present the design of a double crosslinking pure CS hydrogel material via the synergic effect of the chemical covalent network, hydrophobic interactions, enhanced intermolecular hydrogen bonding and the formation of the CS crys...
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#1Xiaoyun Liu (CAS: Chinese Academy of Sciences)H-Index: 7
#2Mingming Hao (CAS: Chinese Academy of Sciences)
Last. Zhijun Zhang (CAS: Chinese Academy of Sciences)H-Index: 56
view all 7 authors...
Abstract Three-dimensional (3D) bioprinting has emerged as a promising approach to fabricate living neural constructs with anatomically accurate complex geometries and spatial distributions of neural stem cells (NSCs) for spinal cord injury (SCI) repair. The NSC-laden 3D bioprinting, however, still faces some big challenges, such as cumbersome printing process, poor cell viability, and minimal cell-material interaction. To address these issues, we have fabricated NSC-laden scaffolds by 3D biopri...
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#1Li Xing (CSU: Central South University)H-Index: 3
#1Xing LiH-Index: 18
Last. Jianwu DaiH-Index: 69
view all 10 authors...
The limited regrowth of transected axons and insufficient regeneration of lost neurons in adult mammals collectively hinder complete spinal cord injury (SCI) repair. Hence, designing an ideal bio-scaffold which could coordinate the regeneration of axons and neurons in situ might be able to effectively facilitate the reconstruction of neural circuits and the recovery of nerve function after complete SCI. In this study, a sponge-like collagen scaffold with good drug release characteristics and goo...
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#1Minmin Song (NU: Nanjing University)H-Index: 2
#1Michael Song (UCSF: University of California, San Francisco)H-Index: 5
Last. Y. Hu
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Emerging evidence demonstrates the important role of circular RNAs (circRNAs) in regulating pathological processes in various diseases including organ fibrosis. Endometrium fibrosis is the leading cause of uterine infertility, but the role of circRNAs in its pathogenesis is largely unknown. Here, we provide the evidence that upregulation of circPTPN12 in endometrial epithelial cells (EECs) of fibrotic endometrium functions as endogenous sponge of miR-21-5p to inhibit miR-21-5p expression and act...
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#1Lulu ZhangH-Index: 2
#2Caixia FanH-Index: 9
Last. Jianwu DaiH-Index: 69
view all 10 authors...
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#1Xiaoyu Xue (CAS: Chinese Academy of Sciences)H-Index: 3
#2Muya Shu (CAS: Chinese Academy of Sciences)H-Index: 2
Last. Jianwu Dai (CAS: Chinese Academy of Sciences)H-Index: 69
view all 14 authors...
Nestin is expressed extensively in neural stem/progenitor cells during neural development, but its expression is mainly restricted to the ependymal cells in the adult spinal cord. After spinal cord injury (SCI), Nestin expression is reactivated and Nestin-positive (Nestin+) cells aggregate at the injury site. However, the derivation of Nestin+ cells is not clearly defined. Here, we found that Nestin expression was substantially increased in the lesion edge and lesion core after SCI. Using a tamo...
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#1Dingyang Liu (CSU: Central South University)H-Index: 10
#2He Shen (CAS: Chinese Academy of Sciences)H-Index: 28
Last. Li Xing (CSU: Central South University)H-Index: 3
view all 9 authors...
Complete transection spinal cord injury (SCI) severely disrupts the integrity of both neural circuits and the microvasculature system. Hence, fabricating a functional bio-scaffold that could coordinate axonal regeneration and vascular reconstruction in the lesion area may emerge as a new paradigm for complete SCI repair. In this study, a photosensitive hydrogel scaffold loaded with collagen-binding stromal cell-derived factor-1a and Taxol liposomes is capable of inducing migration of endothelial...
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