Kousei Ito
Chiba University
Organic anionInternal medicineEndocrinologyMitochondrial permeability transition poreTransporterMolecular biologyChemistryIn vitroIn vivoHepatocyteLiver injuryBile acidDrugMitochondrionMultidrug resistance-associated protein 2ATP-binding cassette transporterGlutathioneBiochemistryMedicineBiologyCell biologyPharmacology
116Publications
29H-index
3,634Citations
Publications 120
Newest
#1Binbin Song (MOE: Chinese Ministry of Education)H-Index: 3
#2Shigeki Aoki (Chiba University)H-Index: 7
Last. Kousei Ito (Chiba University)H-Index: 29
view all 6 authors...
Abstract null null Based on recent genome-wide association studies, abacavir-induced hypersensitivity is highly associated with human leukocyte antigen (HLA)-B*57:01 allele. However, the underlying mechanism of this occurrence is unclear. To investigate the underlying mechanism, we developed HLA-B*57:01 transgenic mice and found that application of abacavir could cause CD8 T cell activation with elevation in PD1 expression; however, severe skin hypersensitivity was not observed. To eliminate the...
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#1Takeshi Susukida (University of Toyama)H-Index: 5
#2Saki Kuwahara (Chiba University)
Last. Kousei Ito (Chiba University)H-Index: 29
view all 6 authors...
Idiosyncratic drug toxicity (IDT) associated with specific human leukocyte antigen (HLA) allotype is a rare and unpredictable life-threatening adverse drug reaction for which prospective mechanistic studies in humans are difficult. Here, we show the importance of immune tolerance for IDT onset and determine whether it is susceptible to a common IDT, HLA-B*57:01-mediated abacavir (ABC)-induced hypersensitivity (AHS), using CD4+ T cell-depleted programmed death-1 receptor (PD-1)-deficient HLA-B*57...
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#1Megumi Kikuya (Chiba University)H-Index: 2
#2Kenta Furuichi (Chiba University)H-Index: 1
Last. Shigeki Aoki (Chiba University)H-Index: 7
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Abstract Tyrosine kinase inhibitors (TKIs) are widely utilized in clinical practice to treat carcinomas, but secondary tumor resistance during chronic treatment can be problematic. AKR1B1 and AKR1B10 of the aldo-keto reductase (AKR) superfamily are highly expressed in cancer cells and are believed to be involved in drug resistance. The aim of this study was to understand how TKI treatment of chronic myelogenous leukemia (CML) cells changes their glucose metabolism and if inhibition of AKRs can s...
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#1Tomoyuki Sato (Chiba University)H-Index: 3
#2Akinori Takemura (Chiba University)H-Index: 2
Last. Kousei Ito (Chiba University)H-Index: 29
view all 3 authors...
Mitochondrial injury contributes to severe drug-induced liver injury. Particularly, mitochondrial permeability transition (MPT) is thought to be relevant to cytolytic hepatitis. However, the mechanism of drug-induced MPT is unclear and prediction of MPT is not adequately evaluated in the preclinical stage. In a previous study, we found that troglitazone, a drug withdrawn due to liver injury, induced MPT via mild depolarization probably resulting from uncoupling. Herein, we investigated whether o...
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#1Taisei Kanamori (Chiba University)H-Index: 1
#2Natumi Miyazaki (Chiba University)H-Index: 1
Last. Hiroto Hatakeyama (Chiba University)H-Index: 33
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Despite progress in the use of hyperthermia in clinical practice, the thermosensitivity of cancer cells is poorly understood. In a previous study, we found that sensitivity to hyperthermia varied between ovarian and uterine cancer cell lines. Upon hyperthermia, glycolytic enzymes decreased in hyperthermia-resistant SKOV3 cells. However, the mechanisms of glycolysis inhibition and their relationship with thermoresistance remain to be explored. In this study, metabolomic analysis indicated the dow...
1 CitationsSource
#1Akinori Takemura (Chiba University)H-Index: 2
#2Simin Gong (Chiba University)H-Index: 1
Last. Kousei Ito (Chiba University)H-Index: 29
view all 8 authors...
Mitochondrial toxicity is an important factor to predict drug-induced liver injury (DILI). Previous studies have focused predominantly on mitochondrial toxicities due to parent forms, and no study has adequately evaluated metabolite-induced mitochondrial toxicity. Moreover, previous studies have used HepG2 cells, which lack many cytochrome P450 (CYP) genes. To overcome this problem, CYP-introduced HepG2 cells were constructed using several gene transfer technologies, including adenoviruses and p...
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#1Sayaka Deguchi (Osaka University)H-Index: 2
#2Tomohiro Shintani (Osaka University)
Last. Hiroyuki MizuguchiH-Index: 76
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#1Yugo Ikeyama (Chiba University)H-Index: 1
#2Tomoyuki Sato (Chiba University)H-Index: 3
Last. Kousei Ito (Chiba University)H-Index: 29
view all 5 authors...
Abstract Recently, mitochondrial dysfunction is thought of as an important factor leading to a drug-induced liver injury (DILI). Our previous reports show that mitochondria-related toxicity, including respiratory chain inhibition (RCI) and reactive oxygen species (ROS) induction, can be detected by the modification of sugar resource substitution and high oxygen condition. However, this in vitro model does not detect mitochondrial permeability transition (MPT)-induced toxicity. Another study with...
3 CitationsSource
#1Takeshi Susukida (University of Toyama)H-Index: 5
#2Shigeki Aoki (Chiba University)H-Index: 7
Last. Kousei Ito (Chiba University)H-Index: 29
view all 6 authors...
Various types of transgenic mice carrying either class I or II human leukocyte antigen (HLA) molecules are readily available, and reports describing their use in a variety of studies have been publ...
2 CitationsSource
#1Shigeki Aoki (Chiba University)H-Index: 7
#2Kengo Shimizu (Chiba University)H-Index: 1
Last. Kousei Ito (Chiba University)H-Index: 29
view all 3 authors...
: Bone homeostasis is maintained by bone remodeling, which involves continuous bone resorption by osteoclasts and bone formation by osteoblasts. Dysregulation of bone turnover, caused by osteoclast overactivation, causes destructive bone diseases. However, the mechanisms underlying the maintenance of osteoclast differentiation and activation are unclear. Herein, we examined the role of autophagy in the maintenance of osteoclast differentiation and maturation. We used in vitro and in vivo assays ...
1 CitationsSource